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2014 | 70 | 04 |
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Activity of some lysosomal enzymes in adrenal cortex during experimental alloxan-induced diabetes mellitus in rabbits

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The aim of the study was the evaluation of changes in the adrenal cortex lysosomal enzymes activity during experimental alloxan-induced diabetes mellitus in rabbits. We checked the activity of acid phosphatase, β-D-galactosidase, N-acetyl-β-D-glucosaminidase (NAGL) and lipase. The study was performed on 124 rabbits divided into five groups: one control and four experimental. Diabetes mellitus was induced by a single injection of 10% alloxan solution into the auricular vein in a dose of 10 mg per kg body weight. Animals from experimental groups were killed in the 21st, 42nd, 90th and 180th days of the study. Adrenal glands were removed. Enzymes activity was assayed by spectrophotometric methods. Changes in free and bound fractions of examined lysosomal enzymes activity were noticed already in the 21st day of diabetes. The most escalated changes were observed in the 42nd day of the study. Performed statistical variance analysis demonstrated statistically highly significant differences for activity of both fractions of NAGL and lipase, as well as for free fraction activity of acid phosphatase and β-D-galactosidase. The obtained data confirmed the influence of diabetes mellitus on changes in the activity of examined lysosomal enzymes in the adrenal cortex.
Opis fizyczny
  • Department of Nephrology, Medical University Lublin, Jaczewskiego 8, 20-954 Lublin, Poland
  • Department of Human Anatomy, Medical University Lublin, Jaczewskiego 8, 20-090 Lublin, Poland
  • Department of Animal Anatomy and Histology University of Life Sciences in Lublin, Akademicka 12, 20-950 Lublin, Poland
  • Department of Human Anatomy, Medical University Lublin, Jaczewskiego 8, 20-090 Lublin, Poland
  • Department of Biochemistry and Toxicology, University of Life Science in Lublin, Akademicka 13, 20-950 Lublin, Poland
  • 1. Adlan M. A., Bonduqulapati L. N., Premawardhana L. D.: Glucose intolerance and diabetes mellitus in endocrine disorders – two case reports and a review. Curr Diabetes Rev. 2010, 5, 266-273.
  • 2. American Diabetes Association: Diagnosis and classification of diabetes mellitus. Diabetes Care 2010, 33, Suppl, 562-569.
  • 3. American Diabetes Association: Gestational diabetes mellitus. Diabetes Care 2003, 26, suppl, 103-105.
  • 4. Biering H., Knappe G., Gerl H., Lochs H.: Prevalance of diabetes in acromegaly and Cushing syndrome. Acta Med. Austr. 2000, 27, 27-31.
  • 5. Burdan F., Siezieniewska Z., Maciejewski R., Burski K., Wojtowicz Z.: Temporary elevation of pancreatic lysosomal enzymes, as a result of the Omeprazol-induced peripancreatic inflammation in male Wistar rats. J. Physiol. Pharmacol. 2000, 51, 463-470.
  • 6. Burdan F., Siezieniewska Z., Maciejewski R., Madej B., Radzikowska E., Wójtowicz Z.: Hepatic lysosomal enzymes activity and liver morphology after short-time omeprazol administration. Exp. Toxic Pathol. 2002, 53, 53-459.
  • 7. Fowler M. J.: Microvascular and macrovascular complications of diabetes. Clin. Diabetes 2008, 26, 77-82.
  • 8. Grankvist K.: Mechanism of alloxan diabetogenicity. UMEA, Sweden 1981.
  • 9. Green J., Casabonne D., Newton R.: Coxsackie B virus serology and Type 1 diabetes mellitus: a systemic review of published case-control studies. Diabet. jMed. 2004 Jun, 6, 507-514.
  • 10. Hage M., Zantout M. S., Azar S. T.: Thyroid disorders and diabetes mellitus. J. Thyroid Res. 2011.
  • 11. Hjelmesaeth J., Sagedal S., Hartmann A., Rollag H., Egeland T., Hagen M., Nordal K. P., Jenssen T.: Asymptomatic cytomegalovirus infection is associated with increased risk of new-onset diabetes mellitus and impaired insulin release after renal transplantation. Diabetologia 2004, 47, 1550-1556.
  • 12. Kelly L., Woodward S. H.: Alteration in the activities of lysosomal glycosidases in human diabetes. Med. Sci. Res. 1988, 16, 491-496.
  • 13. Koike N., Hatori T., Imaizumi T., Harada N., Fukuda A., Takasaki K., Iwamoto Y.: Malignant glucagonoma of the pancreas diagnoses through anemia and diabetes mellitus. J. Hepatobiliary Pancreat Surg. 2003, 10, 101-105.
  • 14. Lowry O. H., Rosenbrouh N. J., Farr A. L., Randal R. J.: Protein measurement with the folin phenol reagent. J. Biol. Chem. 1951, 193, 265-275.
  • 15. Maciejewski R., Burdan F., Hermanowicz-Dryka T., Wójcik K., Wójtowicz Z.: Changes in the activity of some lysosomal enzymes and in the fine structure of submandibular gland due to experimental diabetes. Acta Physiol. Hung. 1999, 86, 127-137.
  • 16. Maciejewski R., Burski K., Baj J., Madej B., Burdan F., Dąbrowski A., Brakowiecki F.: Changes in pancreatic lysosomal enzymes activity as the potential factors leading to diabetic enteropathy. J. Physiol. Pharmacol. 2001, 52, 823-834.
  • 17. Maier A. B.: Cytomegalovirus seropositivity is associated with glucose regulation in the oldest old. Results from the Leiden 85-plus study. Imm. Ageing 2012, 9, 18.
  • 18. Novikoff A. B.: Lysosomes in the physiology and pathology of cells. Contribution of staining methods, [in:] Reuck A. V. S., Churchil J. A. (eds): Ciba Foundation Symposium on Lysosomes, Boston: Little Brown 1963.
  • 19. Pennock C. A.: Lysosomal storage disorders, [in:] Holton J. B. (ed.): The inherited metabolic diseases. Churchill Livingstone, London 1987, 59-95.
  • 20. Resmini E., Minuto F., Coalo A., Ferone D.: Secondary diabetes associated with principal endocrinopathies: the impact of new treatment modalities. Acta Diabetol. 2009, 46, 85-95.
  • 21. Rodrigues T. C., Costenaro F., Fedrizzi D., Oliveira M. D., de Lima P., Boschi V., Czepiewski M. A.: Diabetes mellitus in a cohort of patients with acromegaly. Arq. Bras. Endocrinol Metabl. 2011, 55/9, 714-719.
  • 22. Taczała S., Wójtowicz Z., Bieniek J., Załuska S., Goral B.: The activity of some lysosomal enzymes of the thymus in rabbits. Ann. Univ. Mariae Curie-Sklodowska Med. 2004, 49, 87-92.
  • 23. Walczak D. A., Calvert D., Jarzembowski T. M., Testa G., Sankary H. N., Thielke J., Oberholzer J., Benedetti E.: Increased risk of post-transplant diabetes mellitus despite early steroid discontinuation in Hispanic kidney transplant recipients. Clin. Transplant. 2005, 19, 527-3.
  • 24. Waters P. J., Flynn M. D., Corrall R. J. M., Pennock C. A.: Increases in plasma lysosomal enzymes in Type 1 (insulin-dependent) diabetes mellitus: relationship to diabetic complications and glycaemic control. Diabetologia 1992, 35, 991-995.
  • 25. Wexler B. C., Mc Murtry J. P.: Resistence of obese and non-obese, spontaneously hypertensive rats to alloxan-induced diabetes. Life Sci. 1983, 12, 33, 1097-103.
  • 26. Wójtowicz Z., Burdan F., Pliszczyńska-Steuden M., Kiś G., Golan J., Błaszczak M.: The activity of pancreatic lysosomal enzymes of rabbit during induced experimental diabetes mellitus. Ann. Univ. Mariae Curie-Sklodowska, Lublin 2005, 60, 767-770.
  • 27. Wójtowicz Z., Dworzański W., Kędzierawska-Kurylcio A., Dworzański J., Kiś J. G., Hermanowicz-Dryka T., Kiś J. Z., Lisiecka J., Jędrzejewska E. A., Burdan F.: Activity of testicular lysosomal enzymes in the course of experimental diabetes in rabbits. Ann. Univ. Mariae Curie-Sklodowska, Pharmacia 2009, 22, 135-140.
  • 28. Wójtowicz Z., Szumiło J., Fronczek A., Przybylski P., Hermanowicz-Dryka T., Kiś J., Burdan F.: Morphology of the aorta in experimentally-induced diabetes in rabbits. Ann. Univ. Mariae Curie-Sklodowska, Sectio D 2007, LXII, 60, 321-327.
  • 29. Xu Ling, Kanasaki K., Kitada M., Koya D.: Diabetic angiopathy and angiogenic defects. Fibrogenesis Tissue Repair 2012, 5, 13.
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