Efektywność otrzymywania podwojonych haploidów pszenżyta jarego z wykorzystaniem kultur pylnikowych

• Autorzy: Slusarkiewicz-Jarzina A. , Ponitka A. , Kaczmarek Z.

Warianty tytułu

EN

The efficiency of the production doubled haploid spring triticale through anther culture

• Języki publikacji: PL

Abstrakty

PL

Badano efektywność uzyskiwania androgenicznych struktur i roślin w kulturach pylnikowych sześciu mieszańców pszenżytajarego. Do indukcji androgenezy zastosowano pożywkę C17 z dodatkiem 2,4-D lub Dicamba i stwierdzono zróżnicowaną efektywność uzyskiwania androgenicznych struktur oraz zielonych roślin, w zależności od zastosowanej auksyny w pożywce. Na pożywce zawierającej 2,4-D uzyskano więcej struktur (75,2%) i roślin (6,4%) w porównaniu z pożywką zawierającą Dicamba, na której otrzymano 62,1% struktur i 3,9% roślin. Poziom ploidalności 170 roślin oznaczono cyto- metrycznie i w zależności od stosowanych auksyn stwierdzono 42,0% spontanicznie podwojonych haploidów uzyskanych na pożywce z 2,4-D oraz 31,7% z Dicamba. Ogółem uzyskano 57,1% linii DH w odniesieniu do otrzymanych roślin, w tym 37,1% stanowiły spontanicznie podwojone haploidy oraz 36,6% linie DH uzyskane w wyniku kolchicynowania haploidów.

EN

The efficiency of obtaining androgenic structures and plants in anther culture of 6 spring triticale hybrids was investigated. C17 medium containing 2,4-D or Dicamba was used for androgenesis induction. Varying efficiency of obtaining androgenic structures and green plants was observed, depending on the auxin in the medium. The medium containing 2,4-D produced more structures (75.2%) and plants (6.4%) compared to the medium containing dicamba, which resulted in 62.1 structures and 3.9 plants. Ploidy level was determined by flow cytometry in 170 plants and showed 42.0% of spontaneously doubled haploids from the medium with 2,4-D and 31.7% from the me¬dium with Dicamba. A total of 57.1% DH lines was obtained from the tested plants, out of which 37.1 % were spontaneously doubled haploids and 36.6% of the DH lines were obtained by colchi- cine treatment of haploids.

• Wydawca: -
• Czasopismo: Zeszyty Naukowe Uniwersytetu Przyrodniczego we Wrocławiu. Rolnictwo
• Rocznik: 2014
• Tom: 109
• Opis fizyczny: s.65-74,tab.,wykr.,bibliogr.

Twórcy

autor

Slusarkiewicz-Jarzina A.

• Zakład Biotechnologii, Instytut Genetyki Roślin, Polska Akademia Nauk w Poznaniu

autor

Ponitka A.

• Zakład Biotechnologii, Instytut Genetyki Roślin, Polska Akademia Nauk w Poznaniu

autor

Kaczmarek Z.

• Zakład Biotechnologii, Instytut Genetyki Roślin, Polska Akademia Nauk w Poznaniu

Bibliografia

• Arzani A., Darvey N.L., 2001. The effect of colchicine on triticale anther-derived plants: microspore pretreatment and haploid plant treatment using a hydroponic recovery system. Euphytica, 122: 235-241.
• Arzani A., Darvey N.L., 2002. Androgenetic response of heterozygous triticale populations using a greenhouse hydroponic system. Euphytica, 127: 53-60.
• Barnabás B., Pfahler P.L., Kovács G., 1991. Direct effect of colchicine on the microspore embry-ogenesis to produce dihaploid plants in wheat (Triticum aestivum L.). Theor. Appl. Genet., 81: 675-678.
• Chu C.C., Wang C.C., Sun C.S., Hsu C., Yin K.C., Chu C.Y., Bi F.Y., 1975. Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources. Sci. Sinica, 18: 659-668.
• González J.M., Jouve N., 2000. Improvement of anther culture media for haploid production in triticale. Cereal Res. Comm., 28: 65-71.
• González J.M., Jouve N., 2005. Microspore development during in vitro androgenesis in triticale. Biol. Plant, 49: 23-28.
• Hassawi D.S., Liang G.H., 1990. Effect of cultivar, incubation temperature, and stage of microspo¬re development on anther culture in wheat i triticale. Plant Breed, 105: 332-336.
• Hassawi D.S., Jiahua Q.I., Liang G.H., 1990. Effect of growth regulator and genotype production of wheat and triticale polyhaploids from anther culture. Plant Breed, 104: 40-45.
• Inagaki M.N., Hash C.T. (1998). Production of haploids in bread wheat, durum wheat and hexa- ploid triticale crossed with pearl millet. Plant Breed, 117: 485-487.
• Immonen S., Robinson J., 2000. Stress treatment and ficoll for improving green plant regeneration in triticale anther culture. Plant Sci., 150: 77-84
• Karsai I., Bedo Z., Hayes P.M., 1994. Effect of induction medium pH and maltose concentration on in vitro androgenesis of hexaploid winter triticale and wheat. Plant Cell Tiss. Org. Cult., 39: 49-53.
• Karsai I., Bedo Z., 1997. Effect of carbohydrate content on the embryoid and plant production in triticale anther culture. Cereal Res. Comm., 25: 109-116.
• Marciniak K., Banaszak Z., Wędzony M., 1998. Effect of genotype, medium and sugar on triticale (xTriticosecale Wittm.) anther culture response. Cereal Res. Comm., 26: 145-151.
• Marcińska I., Wędzony M., 2002. Effect of physical, physiological and genetic factors on callus in-duction, differentiation and regeneration of winter triticale (xTriticosecale Wittm.). Cereal Res. Comm., 30: 63-68.
• Muranty H., Sourdille P., Bernard S., Bernard M., 2002. Genetic characterization of spontaneous diploid androgenetic wheat and triticale plants. Plant Breed, 121: 470-474.
• Navarro-Alvarez W., Baenzi Ger P.S., Eskridge K.M., Hugo M., Gustafson D.V., 1994. Addition on colchicine to wheat anther culture media to increase doubled haploid plant production. Plant Breed, 112: 192-198.
• Oleszczuk S., Sowa S., Zimny J., 2004. Direct embryogenesis and green plant regeneration from isolated microspores of hexaploid triticale (x Triticosecale Wittmack) cv. Bogo. Plant Cell Rep., 22: 885-893.
• Oleszczuk S., Rabiza-Swider J., Zimny J., Łukaszewski A.J., 2011. Aneuploidy among androgenic progeny of hexaploid triticale (X Triticosecale Wittmack). Plant Cell Rep., 30/4: 575-586.
• Ouynang J.W., Liang H., Jia S.E., Zhang C., Zhao T.H., He L.Z., Jia X., 1994. Studies on the chro-mosome doubling of wheat pollen plants. Plant Sci., 98: 209-214.
• Ponitka A., Ślusarkiewicz-Jarzina A., Wędzony M., Marcińska I., Woźna J., 1999. The influence of various in vitro culture conditions on androgenetic embryo induction and plant regeneration from hexaploid triticale (X Triticosecale Wittm.). J. Appl. Genet., 40: 165-174.
• Ponitka A., Ślusarkiewicz-Jarzina A., 2011. Otrzymywanie spontanicznych i indukowanych linii podwojonych haploidów pszenżyta ozimego z wykorzystaniem kultur pylnikowych. Biul. IHAR, 260/261: 183-191.
• Pratap A., Sathi G.S., Chaudhary H.K., 2005. Relative efficiency of different Gramineae genera for haploid induction in triticale and triticale x wheat hybrids through the chromosome elimi¬nation technique. Plant Breed, 124: 147-153.
• Redha A., Attia T., Buter B., Saisingtong S., Stamp P., Schmid J.E., 1998. Improved production of doubled haploids by colchicine application to wheat (Triticum aestivum L.) anther culture. Plant Cell Rep., 17: 974-979.
• Sharma C.C., Wang W.C., Sapra V.T., 1982. Effect of genotype, media and temperature pretreat- ment on callus initiation in triticale, wheat and rye anther cultures. Cereal Res. Comm., 10: 143-150.
• Ślusarkiewicz-Jarzina A., Ponitka A., 1997. Effect of genotype and media composition on em- bryoid induction and plant regeneration from anther culture in triticale. J. Appl. Genet., 38: 253-258.
• Ślusarkiewicz-Jarzina A., Ponitka A., 2003. Efficient production of spontaneous and induced dou¬bled haploid triticale plants derived from anther culture. Cereal Res. Comm., 31: 289-296.
• Tuvesson S., Liungberg N., Johansson N., Karlsson K.E., Suis L.W., Josset J.P. 2000., Large-scale production of wheat and triticale double haploids through the use of a single-anther culture method. Plant Breed, 119: 455-459.
• Warzecha R., Sowa S., Salak-Warzecha K., Oleszczuk S., Śliwińska E., Zimny J., 2005. Doubled haploids in production of male sterility maintaining triticale (xTriticosecale Wittmack) lines. Acta Physiol. Plant, 27: 245-250.
• Wang P., Chen Y., 1983. Preliminary study on production of high of pollen H2 generation in winter wheat grown in the field. Acta Agron. Sin., 9: 283-284.
• Wędzony M., Marcińska I., Ponitka A., Ślusarkiewicz-Jarzina A., Woźna J. (1998). Production of doubled haploids in triticale (x Triticoseale Wittm.) by means of crosses with maize (Zea mays L.) using picloram and dicamba. Plant Breed, 117: 211-215.
• Zamani G., Kovacs E., Gouli-Vavdinoudi D., Roupakias G., Barnabas B., 2000. Regeneration of fertile doubled haploid plants colchicine-supplemented media in wheat anther culture. Plant Breed, 119: 461-465.
• Zar J., Biostatistical Analysis. Prentice-Hall, Inc., New Jersey, 1984.
• Zhuang J.J., Xu J., 1983. Increasing differentiation frequencies in wheat pollen callus [in:] Cell and Tissue Culture Techniques for Cereal Crop Improvement, Science Press. Beijing: 431.