Improved zinc tolerance of tobacco by transgenic expression of an allene oxide synthase gene from hexaploid wheat

• Autorzy: Liu H.-H. , Wang Y.-G. , Wang S.-P. , Li H.-J. , Xin Q.-G.
• Języki publikacji: EN

Abstrakty

EN

Allene oxide synthase (AOS, EC 4.2.1.92) is the first specific jasmonate biosynthetic pathway gene. In this study, a full-length cDNA of AOS gene was cloned from common wheat nannong 9918. The gene contains an open reading frame (1,446 bp) encoding 418 amino acids. Comparative and bioinformatic analysis revealed that the deduced protein of TaAOS was highly homologous to AOSs from other plant species. The transcript of TaAOS was found to be abundantly expressed in the flag leaves, and was up-regulated by the inoculation of B. Graminis (DC.) E.O. Speer f. sp. Tritici (Bgt) in wheat leaves. In addition, it was also induced by high concentration of NaCl and ZnCl₂. When TaAOS was overexpressed in tobacco leaves via Agrobacterium tumefaciens infection, the transgenic tobacco plants displayed stronger tolerance to ZnCl₂ stress compared to transgenic GFP plants. Taken together, the above facts demonstrated that TaAOS may play a role in response to diverse stresses in plants.

Słowa kluczowe

EN

zinc; plant tolerance; tobacco; transgenic plant; gene; hexaploid; wheat

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2014
• Tom: 36
• Numer: 09
• Opis fizyczny: p.2433-2440,fig.,ref.

Twórcy

autor

Liu H.-H.

• Postdoctoral Research Center of Shandong Shengfeng Seeds Co., Ltd, 272400 Jiaxiang, Shandong, China

autor

Wang Y.-G.

• Institute of Genetic and Developmental Biology, Chinese Academy of Sciences, 100101 Beijing, China

autor

Wang S.-P.

• Shandong Shengfeng Seeds Co., Ltd, 272400 Jiaxiang, Shandong, China

autor

Li H.-J.

• Shandong Shengfeng Seeds Co., Ltd, 272400 Jiaxiang, Shandong, China

autor

Xin Q.-G.

• Yantai Academy of Agricultural Sciences, 265500 Yantai, China

Bibliografia

• Agrawal GK, Rakwal R, Jwa NS, Han KS, Agrawal VP (2002) Molecular cloning and mRNA expression analysis of the first rice jasmonate biosynthetic pathway gene allene oxide synthase. Plant Physiol Biochem 40:771–782
• Arnon DI (1949) Copper enzymes in isolated chloroplasts polyphenoloxidase in Beta vulgaris. Plant Physiol 24:1–15
• Azooz MM, Youssef MM, Al-Omair MA (2011) Comparative evaluation of Zinc and Lead and their synergistic effects on growth and some physiological responses of Hassawi Okra (Hibiscus esculentus) seedlings. Am J Plant Physiol 6:269–282
• Chen PD, Zhang SZ, Wang XE, Wang SL, Zhou B, Feng G, Liu DJ (2002) New wheat variety nannong 9918 with high yield and powdery mildew resistance. J Nanjing Agric Univ 25:105–106
• Creelman RA, Mullet JE (1997) Biosynthesis and action of jasmonates in plants. Annu Rev Plant Physiol Mol Biol 48:355–381
• Garg N, Aggarwal N (2011) Effects of interactions between cadmium and lead on growth, nitrogen fixation, phytochelatin, and glutathione production in mycorrhizal Cajanus cajan (L.) millsp. J Plant Growth Regul 30:286–300
• Higo K, Ugawa Y, Iwamot M, Korenaga T (1999) Plant cis-acting regulatory DNA elements (PLACE) database: 1999. Nucleic Acids Res 27:297–300
• Howe GA, Lee GI, Itoh A, Li L, DeRocher AE (2000) Cytochrome P450-dependent metabolism of oxylipins in tomato: cloning and expression of allene oxide synthase and fatty acid hydroperoxidelyase. Plant Physiol 123:711–724
• Ishiga Y, Inagaki Y, Toyoda K, Shiraishi T, Ichinose Y (2003) Expression of allene oxide synthase and allene oxide cyclase in the interactions between pea and fungal pathogens. J Gen Plant Pathol 69:351–357
• Jia JZ, Zhao SC, Kong XY, Li YR, Zhao GY, He WM, Appels R, Pfeifer M, Tao Y, Zhang XY, Jing RL, Ma YZ, Gao LF, Gao C, Spannagl M, Mayer KFX, Li D, Pan SK, Zheng FY, Hu Q, Xia XC, Li JW, Liang QS, Chen J, Wicker T, Gou CY, Kuang HH, He GY, Luo YD, Keller B, Xia QJ, Lu P, Wang JY, Zou HF, Zhang RZ, Xu JY, Gao JL, Middleton C, Quan ZW, Liu GM, Wang J, International Wheat Genome Sequencing Consortium, Yang HM, He ZH, Mao L, Wang J (2013) Aegilops tauschii draft genome sequence reveals a gene repertoire for wheat adaptation. Nature 496:91–95
• Jiang KJ, Pi Y, Hou R, Zeng HN, Huang ZS, Zhang Z, Sun XF, Tang KX (2009a) Molecular cloning and expression profiling of the first specific jasmonate biosynthetic pathway gene allene oxide synthase from Lonicera japonica. Mol Biol Rep 36:487–493
• Jiang KJ, Pi Y, Huang ZS, Hou R, Zhang Z, Lin J, Sun XF, Tang KX (2009b) Molecular cloning and mRNA expression profiling of the first specific jasmonate biosynthetic pathway gene allene oxide synthase from Hyoscyamus niger. Russ J Genet 45:430–439
• Kongrit D, Jisaka M, Iwanaga C, Yokomichi H, Katsube T, Nishimura K, Nagaya T, Yokota K (2007) Molecular cloning and functional expression of soybean allene oxide synthases. Biosci Biotechnol Biochem 71:491–498
• Laudert D, Weiler EW (1998) Allene oxide synthase: a major control point in Arabidopsis thaliana octadecanoid signalling. Plant J 15:675–684
• Laudert D, Pfannschmidt U, Lottspeich F, Holländer-Czytko H, Weiler EW (1996) Cloning, molecular and functional characterization of Arabidopsis thaliana allene oxide synthase (CYP74), the first enzyme of the octadecanoid pathway to jasmonates. Plant Mol Biol 31:323–335
• Laudert D, Schaller F, Weiler EW (2000) Transgenic nicotiana tabacum and Arabidopsis thaliana plants overexpressing allene oxide synthase. Planta 211:163–165
• Li XP, Zhao XQ, He X, Zhao GY, Liu DC, Zhang AM, Zhang XY, Tong YP, Li ZS (2011) Haplotype analysis of the genes encoding glutamine synthetase plastic isoforms and their association with nitrogen-use-and yield-related traits in bread wheat. New Phytol 189:449–458
• Ling HQ, Zhao SC, Liu DC, Wang JY, Sun H, Zhang C, Fan HJ, Li D, Dong LL, Tao Y, Gao C, Wu HL, Li YW, Cui Y, Guo XS, Zheng SS, Wang B, Yu K, Liang QS, Yang WL, Lou XY, Chen J, Feng MJ, Jian JB, Zhang XF, Luo GB, Jiang Y, Liu JJ, Wang ZB, Sha YH, Wang XJ, Liu X, Wang FM, Yang YP, An XL, Dong ZY, Zhang KP, Zhang XQ, Luo MC, Dvorak J, Tong YP, Wang J, Yang HM, Li ZS, Wang DW, Zhang AM, Wang J (2013) Draft genome of the wheat A-genome progenitor Triticum urartu. Nature 496:87–90
• Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-DeltaDeltaC(T)) method. Methods 25:402–408
• Lu X, Zhang FY, Jiang WM, Lin XY, Chen YF, Shen Q, Wang T, Wu SY, Sun XF, Tang KX (2012) Characterization of the first specific jasmonate biosynthetic pathway gene allene oxide synthase from Artemisia annua. Mol Biol Rep 39:2267–2274
• Maucher H, Hause B, Feussner I, Ziegler J, Wasternack C (2000) Allene oxide synthases of barley (Hordeum vulgare cv. Salome): tissue specific regulation in seedling development. Plant J 21:199–213
• McConn M, Creelman RA, Bell E, Mullet JE, Browse J (1997) Jasmonate is essential for insect defense in Arabidopsis. PNAS 94:5473–5477
• Mochida K, Yoshida T, Sakurai T, Ogihara Y, Shinozaki K (2009) TriFLDB: a database of clustered full-length coding sequences from triticeae with applications to comparative grass genomics. Plant Physiol 150:1135–1146
• Parchmann S, Gundlach H, Mueller MJ (1997) Induction of 12-oxophytodienoic acid in wounded plants and elicited plant cell cultures. Plant Physiol 115:1057–1064
• Saghai-Maroof MA, Soliman KM, Jorgensen RA, Allard RW (1984) Ribosomal DNA space length polymorphisms in barley: mendelian inheritance, chromosomal locations and population dynamics. PNAS 81:8014–8018
• Shen WJ, Forde BG (1989) Efficient transformation of Agrobacterium spp. by high voltage electroporation. Nucleic Acids Res 17:8385
• Singla-Pareek SL, Yadav SK, Pareek A et al (2006) Transgenic tobacco overexpressing glyoxalase pathway enzymes grow and set viable seeds in zinc-spiked soils. Plant Physiol 140:613–623
• Siqueira-Júnior CL, Jardim BC, Urményi TP, Vicente AC, Hansen E, Otsuki K, da Cunha M, Madureira HC, de Carvalho DR, Jacinto T (2008) Wound response in passion fruit (Passiflora f. edulis flavicarpa) plants: gene characterization of a novel chloroplast-targeted allene oxide synthase up-regulated by mechanical injury and methyl jasmonate. Plant Cell Rep 27:387–397
• Sivasankar S, Sheldrick B, Rothstein SJ (2000) Expression of allene oxide synthase determines defense gene activation in tomato. Plant Physiol 122:1335–1342
• Song WC, Brash AR (1991) Purification of an allene oxide synthase and identification of the enzyme as a cytochrome P450. Science 253:781–784
• Stumpe M, Feussner I (2006) Formation of oxylipins by CYP74 enzymes. Phytochem Rev 3:347–357
• Sun YF, Xie ZM, Li J, Xu JM, Chen ZL, Naidu R (2006) Assessment of toxicity of heavy metal contaminated soils by the toxicity characteristic leaching procedure. Environ Geochem Health 28:73–78
• Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739
• Wang CX, Avdiushko S, Hildebrand DF (1999) Overexpression of a cytoplasm-localized allene oxide synthase promotes the wound-induced accumulation of jasmonic acid in transgenic tobacco. Plant Mol Biol 40:783–793
• Wu JS, Chong K, Xu YY, Tan KH (2004) Cloning and characteristics of an allene oxide synthase Gene (TaAOS) of winter wheat. J Plant Physiol Mol Biol 30:413–420
• Wu JJ, Chen GT, Li YQ, Jia X (2012) Expression analysis of allene oxide synthase gene from Lonicera japonica thunb. Lishizhen Med Mater Med Res 23:945–947

Identyfikatory

DOI

10.1007/s11738-014-1616-7