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2014 | 36 | 09 |
Tytuł artykułu

Improved zinc tolerance of tobacco by transgenic expression of an allene oxide synthase gene from hexaploid wheat

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Allene oxide synthase (AOS, EC 4.2.1.92) is the first specific jasmonate biosynthetic pathway gene. In this study, a full-length cDNA of AOS gene was cloned from common wheat nannong 9918. The gene contains an open reading frame (1,446 bp) encoding 418 amino acids. Comparative and bioinformatic analysis revealed that the deduced protein of TaAOS was highly homologous to AOSs from other plant species. The transcript of TaAOS was found to be abundantly expressed in the flag leaves, and was up-regulated by the inoculation of B. Graminis (DC.) E.O. Speer f. sp. Tritici (Bgt) in wheat leaves. In addition, it was also induced by high concentration of NaCl and ZnCl₂. When TaAOS was overexpressed in tobacco leaves via Agrobacterium tumefaciens infection, the transgenic tobacco plants displayed stronger tolerance to ZnCl₂ stress compared to transgenic GFP plants. Taken together, the above facts demonstrated that TaAOS may play a role in response to diverse stresses in plants.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
36
Numer
09
Opis fizyczny
p.2433-2440,fig.,ref.
Twórcy
autor
  • Postdoctoral Research Center of Shandong Shengfeng Seeds Co., Ltd, 272400 Jiaxiang, Shandong, China
autor
  • Institute of Genetic and Developmental Biology, Chinese Academy of Sciences, 100101 Beijing, China
autor
  • Shandong Shengfeng Seeds Co., Ltd, 272400 Jiaxiang, Shandong, China
autor
  • Shandong Shengfeng Seeds Co., Ltd, 272400 Jiaxiang, Shandong, China
autor
  • Yantai Academy of Agricultural Sciences, 265500 Yantai, China
Bibliografia
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Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-2dac9f24-1ee5-48b7-b80b-27b0ed23c53d
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