Detection of Toxoplasma gondii from meat and meat products by the nested-PCR method and its relationship with seroprevalence in slaughtered animals

• Autorzy: Ergin S. , Ciftcioglu G. , Midilli K. , Issa G. , Gargili A.
• Języki publikacji: EN

Abstrakty

EN

Toxoplasma gondii DNA was detected at rates of 2%, 6%, 4.17%, 20%, and 19% in 50 bovine brains, 50 bovine muscles, 120 ovine brains, 20 ovine muscles, and in 100 fermented sausage samples, respectively. Sequenced positive samples showed 100% identity with T. gondii RH strain. Seropositivity rates were detected as 24% in cattle and 25% in sheep slaughtered in local slaughterhouses. The detection of the parasite in uncooked meat and commercial meat products, and the high ratio of seropositive slaughtered animals, emphasise that the risk still exists for food-transmitted toxoplasmosis. This is the first report from Turkey describing the detection of Toxoplasma gondii in food using the molecular method.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2009
• Tom: 53
• Numer: 4
• Opis fizyczny: p.657-661,ref.

Twórcy

autor

Ergin S.

• Department of Microbiology and Clinical Microbiology, Faculty of Medicine, Istanbul University Cerrahpasa, 34098 Istanbul, Turkey

autor

Ciftcioglu G.

• Department of Food Hygiene and Technology, 3Department of Parasitology, Faculty of Veterinary Medicine, Istanbul University, 34850 Istanbul, Turkey
• Institute for the Protection and Security of the Citizen, Joint Research Centre, European Commission, 21020 Ispra, Italy

autor

Midilli K.

• Department of Microbiology and Clinical Microbiology, Faculty of Medicine, Istanbul University Cerrahpasa, 34098 Istanbul, Turkey

autor

Issa G.

• Department of Food Hygiene and Technology, 3Department of Parasitology, Faculty of Veterinary Medicine, Istanbul University, 34850 Istanbul, Turkey

autor

Gargili A.

• Institute for the Protection and Security of the Citizen, Joint Research Centre, European Commission, 21020 Ispra, Italy

Bibliografia

• 1. Aslan G., Babür C.: Toxoplasma gondii seroprevalence for sheep, cows, and abattoir workers in Sanlıurfa. J Turkish Microbiol Soc 2002, 32, 102-105.
• 2. Aspinall T.V., Marlee D., Hyde J.E., Sims P.F.G.: Prevalence of Toxoplasma gondii in commercial meat products as monitored by polymerase chain reaction - food for thought? Int J Parasitol 2002, 32, 1193-1199.
• 3. Babür C., Inci A., Karaer Z.: Detection on seropositivity of Toxoplasma gondii in sheep and goats in around of Çankırı using Sabin-Feldman dye test. Acta Parasitol Turcica 1997, 21, 409-412.
• 4. Babür C., Karaer Z.: Isolation and seroincidence studies of Toxoplasma gondii infection in sheep. Acta Parasitol Turcica 1997, 21, 293-299.
• 5. Baril L., Ancelle T., Goulet V., Thulliez P., Tirard-Fleury V., Carme B.: Risk factors for Toxoplasma infection in pregnancy: a case-control study in France. Scand J Infect Dis 1999, 31, 305-309.
• 6. Bekele T., Kasalı O.B.: Toxoplasmosis in sheep, goats and cattle in central Ethiopia. Vet Res Commun 1989, 13, 371-375.
• 7. Bobic B., Jevremovic I., Marinkovic J.: Risk factors for Toxoplasma infection in a reproductive age female population in the area of Belgrade, Yugoslavia. Eur J Epidemiol 1998, 14, 605-610.
• 8. Cook A.J., Gilbert R.E., Buffolano W., Zufferey J., Petersen E., Jenum P.A., Foulon W., Semprini A.E.: Sources of Toxoplasma infection in pregnant women: European multicentre case-control study. Br Med J 2000, 321, 142-147.
• 9. Despommier D.D., Gwadz R.W., Hotez P.J., Knirsch C.A.: Toxoplasma gondii. In: Parasitic Diseases. The protozoa, Apple Trees Productions, New York, 2000, pp. 68-76,
• 10. Dubey J.P.: Toxoplasmosis. J Am Vet Med Assoc 1994, 205, 1593-1598.
• 11. Dubey J.P.: Sources of Toxoplasma gondii infection in pregnancy. Br Med J 2009, 321, 127-128.
• 12. Dubey J.P., Sundar N., Hill D., Velmurugan G.V., Bandini L.A., Kwok O.C.H., Majumdar D., Su C.: High prevalence and abundant atypical genotypes of Toxoplasma gondii isolated from lambs destined for human consumption in the USA. Int J Parasitol 2008, 38, 999-1006.
• 13. Esteban-Redondo I., Maley S.W., Thomson K., Nicoll S., Wright S., Buxton D., Innes E.A.: Detection of Toxoplasma gondii in tissues of sheep and cattle following oral infection. Vet Parasitol 1999, 86, 155-171.
• 14. Gaffuri A., Giacometti M., Tranquillo V.M., Magnino S., Cordioli P., Lanfranchi P.J.: Serosurvey of roe deer, chamois and domestic sheep in the central Italian Alps. J Wildl Dis 2006, 42, 685-690.
• 15. Gondim L.F.P., Barbosa H.V., Riberio C.H.A., Saeki H.: Serological survey of antibodies to Toxoplasma gondii in goats, sheep, cattle and water buffaloes in Bahia State, Brazil. Vet Parasitol 1999, 82, 273-276.
• 16. Hall T.A.: Bioedit: a user-friendly biological sequence alignment editor and analysis program for Windows and 95/98/NT, Nucleic Acids Symposium, 1999, pp. 95-98.
• 17. Hashemi-Fesharki R.: Seroprevalence of Toxoplasma gondii in cattle, sheep and goats in Iran. Vet Parasitol 1996, 61, 1-3.
• 18. Huong L.T.T., Ljungstrom B.L., Uggla A., Bjorkman C.: Prevalence of antibodies to Neospora caninum and Toxoplasma gondii in cattle and water buffaloes in southern Vietnam. Vet Parasitol 1998, 75, 53-57.
• 19. Kapperud G., Jenum P.A., Stray-Pedersen B.: Risk factors for Toxoplasma gondii infection in pregnancy. Results of a prospective case-control study in Norway. Am J Epidemiol 1996, 144, 405-412.
• 20. Kocabeyoglu O., Yergök Y.Z., Emekdas G., Kosan E., Birinci I., Diler M.: Toxoplasma IgG and IgM antibodies prevalance in pregnant women. Acta Parasitol Turcica 1996, 20, 149-153.
• 21. Kravetz J.D., Federman D.G.: Toxoplasmosis in pregnancy. Am J Med 2005, 118, 212-216.
• 22. Kravetz J.D., Federman D.G.: Prevention of toxoplasmosis in pregnancy: knowledge of risk factors. Infect Dis Obstet Gynecol 2005, 13, 161-165.
• 23. Mc Donald H.: Eating undercooked meat contributed to approximately 50% of cases of acute Toxoplasma infection in pregnancy. Evid base Obstet Gynecol 2001, 3, 63-64.
• 24. Ohshima A.S., Tsubota N., Hiraoka K.: Latex agglutination microtiter test for diagnosis of Toxoplasma infection in animals. Zentralblat Bacteriol Hygien I Abteilung Original A 1981, 250, 376-382.
• 25. Oncel T., Vural G., Babür C., Kılıc S.: Detection of Toxoplasma gondii seropositivity in sheep in Yalova by Sabin Feldman dye test and latex agglutination test. Acta Parasitol Turcica 2005, 29, 10-12.
• 26. Ozçelik S., Guler H., Saygı G., Poyraz, O.: Investigation of anti-Toxoplasma gondii antibodies in women who keep pets or eat raw meat. Acta Parasitol Turcica 1996, 20, 155-158.
• 27. Polat E., Aslan M., Isenkul R., Aygun G., Aksın N., Cepni I., Altas K.: Investigation of anti-Toxoplasma gondii IgM and IgG antibodies in pregnant women by ELISA. Acta Parasitol Turcica 2002, 26, 350-351.
• 28. Puije W.N.A., Bosompem K.M., Canacoo E.A., Wastling J.M., Akanmori B.D.: The prevalence of anti- Toxoplasma gondii antibodies in Ghanaian sheep and goats. Acta Tropica 2000, 76, 21-26.
• 29. Samad M.A., Rahman K.B., Halder A.K.: Seroprevalence of Toxoplasma gondii in domestic ruminants in Bangladesh. Vet Parasitol 1993, 47, 157- 159.
• 30. Sharif M., Gholami S.H., Ziaei H., Daryani A., Laktarashi B., Ziapour S.P., Rafiei A., Vahedi M.: Seroprevalence of Toxoplasma gondii in cattle, sheep and goats slaughtered for food in Mazandaran province, Iran, during 2005. Vet J 2007, 174, 422-424.
• 31. Tenter A.M., Heckeroth A.R., Weiss L.M.: Toxoplasma gondii: from animals to humans. Int J Parasitol 2000, 30, 1217-1258.
• 32. Tsubota N., Hiraoka K., Sawada Y., Ohshima S., Hoshino M.: Studies on latex agglutination test for toxoplasmosis. 3. Evaluation of the microtiter test as a serologic test for toxoplasmosis in some animals. Jap J Parasitol 1977, 26, 291-298.
• 33. Yildiz K., Babür C., Kılıc S., Aydenizöz M., Dalkılıç I.: Investigation of anti-Toxoplasma gondii antibodies in slaughtered sheep and cattle as well as in workers in the abattoir of Kırıkkale. Acta Parasitol Turcica 2000, 24, 180-185.
• 34. Yigit N., Aktas A.E., Uslu H., Aydın F., Babacan M.: Investigation of Toxoplasma gondii antibodies in the sera of patients suspected of having toxoplasmosis coming to the Department of Microbiology of the Faculty of Medicine, Atatürk University. Acta Parasitol Turcica 2000, 24, 22-24.
• 35. Warnekulasuriya M.R., Johnson J.D., Hollimon R.E.: Detection of Toxoplasma gondii in cured meats. Int J Food Microbiol 1998, 45, 211-215.