Restoration of coelomocytes in the earthworm Dendrobaena veneta

• Autorzy: Klimek M. , Kruk J. , Plytycz B.
• Języki publikacji: EN

Abstrakty

EN

We have previously shown that Dendrobaena veneta belongs to the earthworm species which possesses two main cohorts of coelomocytes, namely amoebocytes and autofluorescent eleocytes, the latter storing moderate amounts of riboflavin. The aim of the present experiments performed at 17°C was to follow the restoration of amoebocytes and eleocytes, as well as riboflavin stores in eleocytes after experimental extrusion of coelomocyte-containing coelomic fluid through the dorsal pores induced by electrostimulation (4.5V, 1 min). The analyses were conducted using a combination of cell counts, flow cytometric detection of eleocytes, and spectrofluorimetric measurements of riboflavin in coelomocyte lysates. It has been found that the depleted coelomocyte system recovers slowly. The number of amoebocytes reaches the level characteristic of that in undisturbed worms in 4 weeks, while the number of elecytes is still below the control level 6 weeks after extrusion. The amount of riboflavin stored in recovering chloragocyte- derived eleocytes is higher than that in mature eleocytes for at least 4 weeks.

• Wydawca: -
• Czasopismo: Acta Biologica Cracoviensia. Series Zoologia
• Rocznik: 2012
• Tom: 54
• Opis fizyczny: p.11-17,fig.,ref.

Twórcy

autor

Klimek M.

• Department of Evolutionary Immunology, Institute of Zoology, Jagiellonian University, Gronostajowa 9, 30-387 Krakow, Poland

autor

Kruk J.

• Faculty of Biochemistry, Biophysics, and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Krakow, Poland

autor

Plytycz B.

• Department of Evolutionary Immunology, Institute of Zoology, Jagiellonian University, Gronostajowa 9, 30-387 Krakow, Poland

Uwagi

PL

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