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2011 | 33 | 2 |
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Functional analysis of tomato LeEIL1 in an Arabidopsis ein2 mutant

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The ETHYLENE-INSENSITIVE3 (EIN3)/EIN3- Like (EIL) EIN3/EILs, novel nuclear proteins, are located at the downstream position of the ethylene signal transduction pathway. LeEIL1, which is expressed in fruit throughout ripening, is key transcription factor in the ethylene signaling pathway in tomato. To reveal its function, the LeEIL1 gene has been transformed into and expressed in the ein2 mutant of Arabidopsis. The expression levels of the transgene in the single copy line, LeEIL1-ein2-b, were higher than those in the multiple-copy line, LeEIL1-ein2-a. The ethylene-insensitive phenotype of the ein2 mutant plants has been partially recovered by expression of LeEIL1. The florescence of LeEIL1-ein2-a and LeEIL1- ein2-b exceeded that of the ein2 mutant but was still less than that of wild type of Arabidopsis. The expression of four pathology-related genes (AtPR3, 4, AtPDF1.2 and AtGST2) has been analyzed in LeEIL1 transgenic ein2 mutant plants. The expression of AtPR3 and AtPR4, which was reduced in the ein2 mutant, was enhanced in the two transgenic Arabidopsis plants. The expression of the AtPDF1.2 gene was unaffected in the two transgenic Arabidopsis lines, the ein2 mutant and wild-type Arabidopsis plants. In addition, the expression level of AtGST2 in transgenic Arabidopsis plants was lower even than that in ein2 mutant and wild-type Arabidopsis plants.
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  • College of Bioengineering, Chongqing University, 400044 Chongqing, People's Republic of China
  • College of Bioengineering, Chongqing University, 400044 Chongqing, People's Republic of China
  • Multidisciplinary Centre for Integrative Biology, School of Biosciences, University of Nottingham, Sutton Bonington, Leicestershire LE12 5RD, UK
  • Beijing Agro-Biotechnology Research Center, 100089 Beijing, People's Republic of China
  • College of Bioengineering, Chongqing University, 400044 Chongqing, People's Republic of China
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