The irp2 and fyuA genes in High Pathogenicity Islands are involved in the pathogenesis of infections caused by avian pathogenic Escherichia coli (APEC)

• Autorzy: Tu J. , Hue T. , Qi K. , Shao Y. , Huang B. , Wang X. , Zhou X.
• Języki publikacji: EN

Abstrakty

EN

Avian pathogenic Escherichia coli (APEC) is a major bacterial infectious disease that may lead to local or systemic infections in chickens with clinical manifestations. The irp2-fyuA gene cluster has been confirmed to be the main genes involved in the synthesis of HPI. The objective of this study was to determine the influence of the irp2 and fyuA genes in the high pathogenicity island (HPI) of avian pathogenic Escherichia coli (APEC) on its pathogenicity by knocking out these genes. The ΔAE17 (lacking irp2) and ΔΔAE17 (lacking irp2 and fyuA) strains of APEC were constructed. The ΔAE17 and ΔΔAE17 strains showed significantly impaired capacity to adhere onto DF-1 cells. The LD50 results indicated that the virulence of the ΔAE17 and ΔΔAE17 strains was decreased in comparison with that of the AE17 strain. We concluded that the knock-out of the core HPI genes weakened APEC adhesion onto DF-1 cells, inhibited transcription of virulence genes, and reduced pathogenicity in chicks. The effects of genetic deletion of irp2 and fyuA on APEC were more severe than those produced by deletion of irp2 only, indicating that irp2 and fyuA co-regulate APEC pathogenicity.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2016
• Tom: 19
• Numer: 1
• Opis fizyczny: p.21-29,fig.,ref.

Twórcy

autor

Tu J.

• College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, PR China

autor

Hue T.

• College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, PR China

autor

Qi K.

• College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, PR China

autor

Shao Y.

• College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, PR China

autor

Huang B.

• College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, PR China

autor

Wang X.

• College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, PR China

autor

Zhou X.

• College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, PR China

Bibliografia

• Antao EM, Wieler LH, Ewers C. (2009) Adhesive threads of extraintestinal pathogenic Escherichia coli. Gut Pathog 1: 22.
• Bach S, Almeida A, Carniel E. (2000) The Yersinia high-pathogenicity island is present in different members of the family Enterobacteriaceae. FEMS Microbiol Lett 183: 289-294.
• Carniel E, Guilvout I, Prentice M. (1996) Characterization of a large chromosomal :high pathogenicity island; in biotype 1B Yersinia enterocolitica. J Bacteriol 178: 6743-6751.
• Fetherston JD, Lillard JJW, Perry RD. (1995) Analysis of the pesticin receptor from Yersinia pestis, role in iron-deficient growth and possible regulation by its siderophore. Bacteriol. 177: 1824-1833.
• Fetherston JD, Perry RD. (1994) The pigmentation locus of Yersinia pestis is flanked by an insertion sequence and includes the structural genes for pesticin sensitivity and HMWP2. Mol Microbiol 13: 697-708.
• Han XG, Bai H, Lei L, et al. (2013) The luxS gene functions in the pathogenesis of avian pathogenic Escherichia coli. Microbial Pathog 6: 21-27.
• Jacobi CA, Gregor S, Rakin A, Heesemann J. (2001) Expression Analysis of the Yersiniabactin Receptor Gene fyuA and the Heme Receptor hemR of Yersinia enterocolitica In Vitro and In Vivo Using the Reporter Genes for Green Fluorescent Protein and Luciferase. Infect Immun 69: 7772.
• Jones CH, Pinkner JS, Nicholes AV, et al. (1993) Fim C is a periplasmic PapD-like chaperone that directs assembly of type 1 pili in bacteria. Proc Natl Acad Sci USA 90: 8397-8401.
• Karch H, Schubert S, ZhangD. et al. (1999) A genomic island, termed high-pathogenicity island, is present in certain non-O157 Shiga toxin-producing Escherichia coli clonal lineages. Infect Immun 67: 5994-6001.
• Kurmanova A, Llorente A, Polesskaya A, et al. (2007) Structural requirements for furin-induced cleavage and aetivation of Shiga toxin. Biochem Biophys Res Commun 357: 144-149.
• Muyrers JP, Zhang Y, Stewart AF. (2001) Recombinogenics engineering-new options for cloning and manipulating DNA. Trends Biochem Sci 26: 325-331.
• Poteete AR. What makes the bacteriophage lambda Red system useful for genetic engineering molecular mechanism and biological function. (2001) FEMS Microbiol Lett 201: 9-14.
• Rakin A, Noelting C, Schubert S, Heesmann J. (1999) Common and specific characteristics of the high-pathogenicity island of Yersinia enterocolitica. Infect Immun 67: 5265-5274.
• Schouler C, Schaeffer B, Bree A, Mora A, Dahbi G, Biet F, Oswalde E, Mainil J, Blanco J, Schouleur MM. (2012) Diagnostic strategy for identifying Avian Pathogenic Escherichia coli based on four patterns of virulence genes. J Clin Microbiol 50: 1673-1678.
• Schouler C, Germon P, Chen YH, He L, Blanco JE, Bree A, Huang SH, Moulin-Schouleur M. (2005) ibeA, a virulence factor of avian pathogenic Escherichia coli. Microbiology 151: 1179-1186.

Identyfikatory

DOI

10.1515/pjvs-2016-0004