Effects of sucrose, formic acid and lactic acid bacteria inoculant on quality, in vitro rumen digestibility and fermentability of drooping wild ryegrass (Elymus nutans Griseb.) silage

• Autorzy: Zhang Q. , Yang H. , Yu Z.
• Języki publikacji: EN

Abstrakty

EN

The aim of the study was to evaluate the effect of sucrose, formic acid and lactic acid bacteria (LAB) inoculant on chemical composition and in vitro rumen digestibility of drooping wild ryegrass (Elymus nutans Griseb.) silage. Fresh drooping wild ryegrass was harvested at boot stage and ensiled with: 1. control (untreated), 2. 20 g · kg−1 sucrose (S), 3. 3 g · kg−1 formic acid (FA) and 4. 0.005 g · kg−1 commercial LAB inoculant (LP). The addition of all three additives increased the Flieg point, and decreased pH and butyric acid content (P < 0.05). There was lower ammonia N content in silages treated with S and FA. On the other hand, LP addition elevated lactic acid content with simultaneous increase of the lactic to acetic acid ratio (P < 0.001). The silages were further anaerobically incubated at 39 °C for 48 h with buffered rumen fluid of lactating cows. All three additives increased volatile fatty acid production (P = 0.008). Addition of LP increased average gas production rate and ammonia N content (P < 0.001). In brief, well fermented drooping wild ryegrass silages were obtained by adding LP or S in comparison to FA. LP can be recommended as a silage additive for preparing well fermented silage, and FA could be applied as a regulator additive to control the level of lactic acid produced during drooping wild ryegrass ensiling.

• Wydawca: -
• Czasopismo: Journal of Animal and Feed Sciences
• Rocznik: 2017
• Tom: 26
• Numer: 1
• Opis fizyczny: p.26-32,fig.,ref.

Twórcy

autor

Zhang Q.

• College of Forestry and Landscape Architecture Guangzhou 510642, South China Agricultural University, P.R. China
• Institute of Grassland Science, College of Animal Science and Technology, China Agricultural University, Beijing100193, P.R. China

autor

Yang H.

• Institute of Grassland Science, College of Animal Science and Technology, China Agricultural University, Beijing100193, P.R. China

autor

Yu Z.

• State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing100193, P.R. China

Bibliografia

• Amer S., Hassanat F., Berthiaume R., Seguin P., Mustafa A.F., 2012. Effects of water soluble carbohydrate content on ensiling characteristics, chemical composition and in vitro gas production of forage millet and forage sorghum silages. Anim. Feed Sci. Technol. 177, 23–29, https://doi.org/10.1016/j.anifeedsci.2012.07.024
• Bolsen K.K., Ashbell G., Weinberg Z.G., 1996. Silage fermentation and silage additives. Asian-Australas. J. Anim. Sci. 9, 483–494, https://doi.org/10.5713/ajas.1996.483
• Broderick G.A., Kang J.H., 1980. Automated simultaneous determination of ammonia and total amino acids in ruminal fluid and in vitro media. J. Dairy Sci. 63, 64–75, https://doi.org/10.3168/jds.S0022-0302(80)82888-8
• Cao Y., Cai Y., Takahashi T., Yoshida N., Tohno M., Uegaki R., Nonaka K., Terada F., 2011. Effect of lactic acid bacteria inoculant and beet pulp addition on fermentation characteristics and in vitro ruminal digestion of vegetable residue silage. J. Dairy Sci. 94, 3902–3912, https://doi.org/10.3168/jds.2010-3623
• Chen M.M., Liu Q.H., Xin G.R., Zhang J.G., 2013. Characteristics of lactic acid bacteria isolates and their inoculating effects on the silage fermentation at high temperature. Lett. Appl. Microbiol. 56, 71–78, https://doi.org/10.1111/lam.12018
• Chen S.-Y., Ma X., Zhang X.-Q., Chen Z.-H., 2009. Genetic variation and geographical divergence in Elymus nutans Griseb. (Poaceae: Triticeae) from West China. Biochem. Syst. Ecol. 37, 716–722, https://doi.org/10.1016/j.bse.2009.12.005
• Contreras-Govea F.E., Muck R.E., Broderick G.A., Weimer P.J., 2013. Lactobacillus plantarum effects on silage fermentation and in vitro microbial yield. Anim. Feed Sci. Technol. 179, 61–68, https://doi.org/10.1016/j.anifeedsci.2012.11.008
• Denek N., Can A., Avci M., Aksu T., Durmaz H., 2011. The effect of molasses-based pre-fermented juice on the fermentation quality of first-cut lucerne silage. Grass Forage Sci. 66, 243–250, https://doi.org/10.1111/j.1365-2494.2011.00783.x
• Fang J., Matsuzaki M., Suzuki H., Cai Y., Horiguchi K.I., Takahashi T., 2012. Effects of lactic acid bacteria and urea treatment on fermentation quality, digestibility and ruminal fermentation of roll bale rice straw silage in wethers. Grassl. Sci.58, 73–78, https://doi.org/10.1111/j.1744-697X.2012.00244.x
• Filya I., Muck R.E., Contreras-Govea F.E., 2007. Inoculant effects on alfalfa silage: fermentation products and nutritive value. J. Dairy Sci. 90, 5108–5114, https://doi.org/10.3168/jds2006-877
• Fu J., Sun Y., Chu X., Xu Y., Hu T., 2014. Exogenous 5-aminolevulenic acid promotes seed germination in Elymus nutans against oxidative damage induced by cold stress. PLoS ONE 9, e107152, https://doi.org/10.1371/journal.pone.0107152
• Guo G., Yuan X., Li L., Wen A., Shao T., 2014. Effects of fibrolytic enzymes, molasses and lactic acid bacteria on fermentation quality of mixed silage of corn and hulless-barely straw in the Tibetan Plateau. Grassl. Sci. 60, 240–246, https://doi.org/10.1111/grs.12060
• Heinritz S.N., Martens S.D., Avila P., Hoedtke S., 2012. The effect of inoculant and sucrose addition on the silage quality of tropical forage legumes with varying ensilability. Anim. Feed Sci. Technol. 174, 201–210, https://doi.org/10.1016/j.anifeedsci.2012.03.017
• Kozelov L.K., Iliev F., Hristov A.N., Zaman S., McAllister T.A., 2008. Effect of fibrolytic enzymes and an inoculant on in vitro degradability and gas production of low-dry matter alfalfa silage. J. Sci. Food Agric. 88, 2568–2575, https://doi.org/10.1002/jsfa.3393
• Krishnamoorthy U., Soller H., Steingass H., Menke K.H., 1991. A comparative study on rumen fermentation of energy supplements in vitro. J. Anim. Physiol. Anim. Nutr. 65, 28–35, https://doi.org/10.1111/j.1439-0396.1991.tb00237.x
• Lima R., Lourenço M., Díaza R.F., Castro A., Fievez V., 2010. Effect of combined ensiling of sorghum and soybean with or without molasses and lactobacilli on silage quality and in vitro rumen fermentation. Anim. Feed Sci. Technol. 155, 122–131, https://doi.org/10.1016/j.anifeedsci.2009.10.008
• Lu B.R., 1993. Meiotic studies of Elymus nutans and E. jacquemontii (Poaceae,Triticeae) and their hybrids with Pseudoroegneria spicata and seventeen Elymus species. Plant Syst. Evol. 186, 193–212, https://doi.org/10.1007/BF00940798
• McDonald P., Henderson A.R., Heron S.J.E., 1991. The Biochemistry of Silage. 2nd Edition. Chalcombe Publications. Marlow (UK)
• McEniry J., King C., O’Kiely P., 2014. Silage fermentation characteristics of three common grassland species in response to advancing stage of maturity and additive application. Grass Forage Sci. 69, 393–404, https://doi.org/10.1111/gfs.12038
• Menke K.H., Steingass H., 1988. Estimation of the energetic feed value obtained by chemical analysis and in vitro gas production using rumen fluid. Anim. Res. Dev. 28, 7–55
• Miao J., Zhang X., Che S., Ma X., Chen Z., Zhong J., Bai S., 2011. Gliadin analysis of Elymus nutans Griseb. from the Qinghai-Tibetan Plateau and Xinjiang, China. Grassl. Sci. 57, 127–134, https://doi.org/10.1111/j.1744-697X.2011.00219.x
• Muck R.E., Filya I., Contreras-Govea F.E., 2007. Inoculant effects on alfalfa silage: in vitro gas and volatile fatty acid production. J. Dairy Sci. 90, 5115–5125, https://doi.org/10.3168/jds.2006-878
• Nadeau E.M.G., Buxton D.R., Russell J.R., Allison M.J., Young J.W., 2000. Enzyme, bacterial inoculant, and formic acid effects on silage composition of orchardgrass and alfalfa. J. Dairy Sci. 83, 1487–1502, https://doi.org/10.3168/jds.S0022-0302(00)75021-1
• Ohyama Y., Morichi T., Masaki S., 1975. The effect of inoculation with Lactobacillus plantarum and addition of glucose at ensiling on the quality of aerated silages. J. Sci. Food Agric. 26, 1001–1008, https://doi.org/10.1002/jsfa.2740260717
• Reynal S.M., Ipharraguerre I.R., Liñeiro M., Brito A.F., Broderick G.A., Clark J.H., 2007. Omasal flow of soluble proteins, peptides, and free amino acids in dairy cows fed diets supplemented with proteins of varying ruminal degradabilities. J. Dairy Sci. 90, 1887–1903, https://doi.org/10.3168/jds.2006-158
• Rymer C., Williams B.A., Brooks A.E., Davies D.R., Givens D.I., 2005. Inter-laboratory variation of in vitro cumulative gas production profiles of feeds using manual and automated methods. Anim. Feed Sci. Technol. 123–124, 225–241, https://doi.org/10.1016/j.anifeedsci.2005.04.029
• Weinberg Z.G., Muck R.E., 1996. New trends and opportunities in the development and use of inoculants for silage. FEMS Microbiol. Rev. 19, 53–68, https://doi.org/10.1111/j.1574-6976.1996.b00253.x
• Yang H.J., Zhuang H., Meng X.K., Zhang D.F., Cao B.H., 2014. Effect of melamine on in vitro rumen microbial growth, methane production and fermentation of Chinese wild rye hay and maize meal in binary mixtures. J. Agric. Sci. 152, 686–696, https://doi.org/10.1017/S0021859613000725
• Zhang Q., Li X.J., Zhao M.M.,Yu Z., 2014a. Isolating and evaluating lactic acid bacteria strains for effectiveness of Leymus chinensis silage fermentation. Lett. Appl. Microbiol. 59, 391–397, https://doi.org/10.1111/lam.12291
• Zhang X.Q., Jin Y.M., Zhang Y.J., Yu Z., Yan W.H., 2014b. Silage quality and preservation of Urtica cannabina ensiled alone and with additive treatment. Grass Forage Sci. 69, 405–414, https://doi.org/10.1111/gfs.12036

Identyfikatory

DOI

10.22358/jafs/68802/2017