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2014 | 14 |
Tytuł artykułu

Media optimization for depolymerization of alginate by Pseudomonas aeruginosa AG LSL-11

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
An agar degrading bacterium Pseudomonas aeruginosa AG LSL-11 was acclimatized to alginate for the production of alginase. Production parameters such as pH, temperature, influence of simple carbohydrates and nitrogen sources, and effect of NaCl on growth and alginase production were carried out. Maximum growth was observed at pH 9.0 and 35 °C, while alginase was produced optimally at pH 9.0 and 30 °C. The alginase produced by Pseudomonas aeruginosa AG LSL-11 was inducible by alginate, and repressed by other simple sugar when supplemented along with alginate in the medium. The bacterium did not require NaCl for growth and production of alginase. The activity staining of partially purified culture supernatant after native PAGE revealed the presence of a single alginase.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
14
Opis fizyczny
p.30-39,fig.,ref.
Twórcy
autor
  • Department of Biochemistry, Gulbarga University, Gulbarga, Karnataka, India
autor
  • Department of Biochemistry, Tumkur University, Tumkur, Karnataka, India
autor
  • Department of Biochemistry, Gulbarga University, Gulbarga, Karnataka, India
Bibliografia
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  • [10] Alexeeva Y. V., Ivanova E. P., Zvaygintseva T. N., Mikhailov V.V., Lett Appl Microbiol. 35 (2002) 343-346.
  • [11] Liyan Li., Xiaolu J., Huashi G., Peng W., Hong G., Appl Biochem Biotechnol 164 (2011) 305-317.
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  • [14] Basawaraj A. Koti, S. Manohar, J. Lalitha. Biotechnol Bioproc Eng. 18 (2013) 333-341.
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  • [17] Basawaraj A. Koti, S. Manohar, J. Lalitha, Preparative Biochemistry & Biotechnology 42 (2012) 364-377.
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-2c92b157-7cb5-481c-a247-5a9d2c364951
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