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2002 | 07 | 2A |
Tytuł artykułu

The cytogenetics and molecular characteristics of a translocated chromosome 1AS.1AL-1DL with a Glu-D1 locus in durum wheat

Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Wheat quality depends directly on the grain protein content and protein composition. High and low molecular weight glutenin subunits play an important role in determining the visco-elastic properties of gluten. In an attempt to improve the breadmaking quality of hexaploid triticale, a fragment of wheat chromosome 1D, containing the Glu-D1 allele encoding the 5+10 subunits, was translocated to the long arm of chromosome 1A by Lukaszewski and Curtis [1], The 1A.1D translocation chromosome was transferred to tetraploid wheat [2], making the Glu-D1 locus available for the improvement of durum wheat. The goal of this study was to evaluate using cytogenetics and molecular approaches the amount of chromatin introgressed in durum wheat. Fluorescence in situ hybridization with total genomic DNA (GISH) of Aegilops squarrosa L. indicated that the translocated chromosome 1A.1D had a terminal 1DL segment of about 35-40% of the recombinant arm length. Several pairs of microsatellite primers from chromosome 1A and 1D were used to genetically characterize the recombinant chromosome. The mapping data indicated that a 1AL segment, at least 150 cM long, was substituted by a 1DL segment with a minimal length of 72 cM, and that the translocation breakpoint was near the 1A centromeric region. The genetic and physical data highlight a substantial discrepancy between the recombinational and physical map distances. We are using a targeted strategy via the Ph pairing manipulation system to generate smali intercalary 1D chromosome segments in a durum wheat background.
Wydawca
-
Rocznik
Tom
07
Numer
2A
Opis fizyczny
p.559-567,fig.,ref.
Twórcy
autor
  • Department of Agro-Forestry and Environmental Biology and Chemistry, University of Bari, Via Amendola 165/A, 70126 Bari, Italy
autor
  • Department of Agro-Forestry and Environmental Biology and Chemistry, University of Bari, Via Amendola 165/A, 70126 Bari, Italy
autor
  • Department of Agro-Forestry and Environmental Biology and Chemistry, University of Bari, Via Amendola 165/A, 70126 Bari, Italy
autor
  • Department of Agro-Forestry and Environmental Biology and Chemistry, University of Bari, Via Amendola 165/A, 70126 Bari, Italy
autor
  • Germplasm Institute, C.N.R., Via Amendola 165/A, 70126 Bari, Italy
autor
  • Germplasm Institute, C.N.R., Via Amendola 165/A, 70126 Bari, Italy
Bibliografia
  • 1. Lukaszewski, A. J. and Curtis, C. A. Transfer of the Glu-D1 gene from chromosome 1D to chromosome 1A in hexaploid triticale. Plant Breed. 112 (1994) 177-182.
  • 2. Blanco, A., Cenci, A., Simeone, R., Pignone, D. and Galasso, I. Characterization and transfer of a recombinant chromosome 1AS.1AL-1DL with the Glu-D1 gene from hexaploid triticale to durum wheat. In: Proc. 4th Int. Triticeae Symposium, Cordoba (Spain), 10-12 September, 2001.
  • 3. Bushuk, W. Aspects of chemical and physical structure of wheat proteins that determine breadmaking quality. In: Hard Wheat: Agronomic, Technological, Biochemical And Genetic Aspects, (Borghi, B. Ed.). Commission of the European Communities, Luxemburg, 1987, 7-17.
  • 4. Payne, P. I. Genetics of wheat storage proteins and the effect of allelic variation on breadmaking quality. Ann. Rev. Plant Physiol. 38 (1987) 141- 153.
  • 5. Lukaszewski, A. J. and Curtis, C. A. Transfer of the Glu-D1 gene from chromosome 1D of bread wheat to chromosome 1R in hexaploid triticale. Plant Breeding 109 (1992) 203-210.
  • 6. Ceoloni, C., Ciaffi, M., Lafiandra, D. and Giorgi, B. Chromosome engineering as a means of transferring 1D storage protein genes from common to durum wheat. In: Proc. 8th Int. Wheat Genet. Symp. (Li, Z. S., Xin, Z. Y., Eds.) Beijing, China, 1995, 159-163.
  • 7. Ceoloni, C., Biagetti, M., Ciaffi, M., Forte, P. and Pasquini, M. Wheat chromosome engineering at the 4x level: the potential of different alien gene transfers into durum wheat. Euphytica 89 (1996) 87-97.
  • 8. Vitellozzi, F., Ciaffi, M., Dominaci, L. and Ceoloni, C. Isolation of a chromosomally engineered durum wheat line carrying the common wheat Glu-D1d allele. Agronomie 17 (1997) 413-419.
  • 9. Schwarzacher, T., Leitch, A. R. and Heslop-Harrison, J. S. DNA-DNA in situ hybridisation-methods for light microscopy. In: Plant Cell Biology: A Practical Approach, (Harris, N., Oparka, K. J. Eds), IRL Press, Oxford University Press, Oxford, 1994, 127-155.
  • 10. Rayburn, A. L. and Gill, B. S. Isolation of a D-genome specific repeated DNA sequence from Aegilops squarrosa. Plant Mol. Biol. Rep. 4 (1986) 102-109.
  • 11. Röder, M. S., Korzun, V., Wendehake, K., Plaschke, J., Tixier, M.-H., Leroy, P. H. and Ganal, M. W. A microsatellite map of wheat. Genetics 149 (1998) 2007-2023.
  • 12. Payne, P. I., Holt, L. M. and Law, C. N. Structural and genetical studies of the high-molecular-weight subunits of wheat glutenin. 1. Allelic variation in subunits amongst varieties of wheat (Triticum aestivum). Theor. Appl. Genet. 60 (1981) 229-236.
  • 13. Lafiandra, D. and Kasarda, D. D. One- and two-dimentional (2-pH) polyacrylamide gel electrophoresis in a single gel. Separation of wheat proteins. Cereal Chem. 62 (1985) 314-319.
  • 14. Galasso, I., Blanco, A., Katsiotis, A., Pignone, D. and Heslop-Harrison, J. S. Genomic organization and phylogenetic relationships in the genus Dasypyrum analysed by Southern and in situ hybridization of total genomic and cloned DNA probes. Chromosoma 106 (1997) 53-61.
  • 15. Mukai, Y., Nakahara, Y. and Yamamoto, M. Simultaneous discrimination of the three genomes in hexaploid wheat by multicolour fluorescence in situ hybridization using total genomic and highly repeated DNA probes. Genome 36 (1993) 489-494.
  • 16. Van Deynze, A. E., Dubcovsky, J., Gili, K. S., Nelson, J. C., Sorrells, M. E., Dvorak, J., Gili, B. S., Lagudah, E. S., McCouch, S. R. and Appels, R. Molecular-genetic maps for group 1 chromosomes of Triticeae species and their relation to chromosomes in rice and oat. Genome 38 (1995) 45-49.
  • 17. Ma, X. F., Ross, K. and Gustafson, J. P. Physical mapping of restriction length polymorphism (RFLP) markers in homoeologous groups 1 and 3 chromosomes of wheat by in situ hybridisation. Genome 44 (2001) 401-412.
  • 18. Pestova, E., Ganal, M. W. and Roder, M. S. Isolation and mapping of microsatellite markers specific for the D genome of bread wheat. Genome 43 (2000) 689-697.
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-2c614bad-c005-4942-971f-518676b19733
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