Locomotor training of spinal rats decreases abundance of GlyR-anchoring gephyrin in the ancle extensor and flexor motoneurons mildly reducing perineuronal nets encapsulating them

• Autorzy: Skup M. , Glowacka A. , Gajewska-Wozniak O. , Czarkowska-Bauch J.
• Języki publikacji: EN

Abstrakty

EN

BACKGROUND AND AIMS: Complete spinal cord transection (SCT) causes reorganization of spinal networks involving changes ofsynaptic terminals abutting on α-motoneurons(MNs).We showed that SCT impoverishes excitatory cholinergic input to ankle extensor but not to flexor MNs and locomotor training leads to its enrichment on both MNs groups (Skup et al. 2012). The opposite effect of training after SCT was found on inhibitory glycinergic (Gly) inputs to MNs. To disclose the impact of SCT and training on postsynaptic components of Gly transmission and on MNs perineuronal nets (PNN), which are inhibitory to synaptic plasticity, and to verify if they respond differently in ankle extensor and flexor MNs. METHODS: GlyR and gephyrin (Geph) were detected immunohistochemically and PNN were visualized with Wisteria floribunda agglutinin (WFA) on sections of L3–6 spinal segments in adult rats 5 weeks after SCT (Th9–10) and after 4 weeks of treadmill training of spinal rats. Extensor and flexor MNs were identified with Diamidine Yellow and Fast Blue respectively, injected intramuscularly. Images acquired in confocal microscope were deconvolved and analyzed with Image-Pro Plus Software. WFA and Geph were quantified in a 3 µm rim around MNs. RESULTS: When all groups of motoneurons were analyzed, no effect of SCT on GlyR and Geph MNs membrane occupancy was detected, but the training decreased GlyR (P<0.05) and Geph (P<0.01) membrane expression to approximately 50% of control. Extensor but not flexor MNs tended to respond to SCT with Geph increase by 22% whereas training decreased it in both to 75%. PNN staining intensity increased after SCT by 75% in extensor and by 44% in flexor MNs (P<0.02) and the training tended to decrease it. CONCLUSIONS: Locomotor training after CST may facilitate reorganization of MN inputs by reducing PNN-encapsulation of MNs and alter MN properties by decreased glycinergic signaling. Supported by grants: NCN 2013/09/B/NZ4/03306, statutory for the Nencki Institute.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2015
• Tom: 75
• Numer: Supl.
• Opis fizyczny: p.S76

Twórcy

autor

Skup M.

• Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warsaw, Poland

autor

Glowacka A.

• Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warsaw, Poland

autor

Gajewska-Wozniak O.

• Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warsaw, Poland

autor

Czarkowska-Bauch J.

• Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warsaw, Poland