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2012 | 68 | 03 |

Tytuł artykułu

Role of apoptotic protease-activating factors (factors: 1, 2, 3) in L-arginine-induced programmed cell death of hepatocytes

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The aim of the study was an immunhistochemical evaluation of the expression of Apaf 1, Apaf 2 and Apaf 3 (apoptotic protease activating factors) in L-arginine, a substrate of nitric oxide (NO) inducing apoptosis of rats’ hepatocytes. The rats used in this experiment were divided into 2 equal groups. Experimental rats received per os L-arginine 40 mg/kg body weight every other day for 2 weeks and were decapitated after 3 weeks of the experiment. Control rats received per os 2 ml of distilled water every other day for 2 weeks and were decapitated after 3 weeks of the experiment. Specimens of the liver taken after decapitation were examined immunohistochemically, using the standard three-step method to detect the immunolocalization of Apaf 1, Apaf 2, Apaf 3. The results of immunohistochemical examinations were subjected to qualitative evaluation based on the intensity of a colour reaction at the antigen-antibody site in the rat liver examined in individual groups. The quantitative evaluation was conducted with the Analysis-pro software. The surface area of cells with a positive colour reaction (+) of antigen-antibody was calculated. The results of the study show that L-arginine, as a donor of exogenous nitric oxide, did not have an apoptotic effect leading by the intrinsic pathway, through the formation of the apoptosome and the activation of caspase 9 in the hepatocytes of rats.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

68

Numer

03

Opis fizyczny

p.172-174,fig.,ref.

Twórcy

autor
  • Department of Histology and Embryology with Laboratory of Experimental Cytology, Medical University, Radziwillowska 11, 20-080 Lublin, Poland
autor

Bibliografia

  • 1.Adams J. M., Cory S.: Apoptosomes: engines for caspase activation. Curr. Opin. Cell. Biol. 2002, 14, 715-720.
  • 2.Budd S. L., Nicholls D. G.: Mitochondria in the life and death of neurons. Essays. Biochem. 1988, 33, 43-52.
  • 3.Chmielewski M., Linke K., Zabel M., Szuber L.: Apoptosis in the liver. Gastroent. Pol. 2003, 10, 453-462.
  • 4.Domiński Z.: Apoptoza: śmierć komórek w życiu organizmów zwierzęcych. Kosmos 1999, 48, 385-396.
  • 5.Garthwaite G., Batchelor A. M., Goodwin D. A., Hewson A. K., Leerning K., Ahmed Z., Cuzner M. L., Garthwaite J.: Pathological implications of iNOS expression in central white matter: an ex vivo study of optic nerves from rats with experimental allergic encephalomyelitis. Eur. J. Neurosci. 2005, 21, 2127-2135.
  • 6. Hibbs J. B. Jr., Taintor R. R., Vavrin Z., Rachlin E. M.: Nitric oxide: a cytotoxic activated macrophage effector molecule. Biochem. Biophys. Res. Commun. 1988, 157, 87-94.
  • 7.Ignarro L. J.: Endothelium derived nitric oxide: actions and properties. FASEB. J. 1989, 3, 31-36.
  • 8.Izdebska M., Grzanka A., Ostrowski M.: Rak pęcherza moczowego - molekularne podłoże genezy i leczenia. Kosmos 2005, 54, 213-220.
  • 9.Kiliańska Z. M., Miskiewicz A.: Kaspazy kregowcow; ich rola w przebiegu apoptozy. Post. Biol. Kom. 2003, 30, 12-15.
  • 10.Lockshin R., Zakeri Z., Tilly J.: When cells die. A comprehensive evaluation of apoptosis and programmed cell death. Wiley-Liss New York 1998, 385-410.
  • 11.Moncada S., Higgs A.: Mechanism of disease: L-arginine-nitric oxide pathway. N. Engl. J. Med. 1994, 329, 2002-2012.
  • 12.Pedrycz A., Zwierzyńska E., Orłowski M., Zając A., Zwierzyński M.: Evaluation of mitochondrial pathway in L-arginine induced apoptosis of rat's hepatocytes. Curr. Probl. Psychiatry. 2011, 12, 213-216.
  • 13.Pedrycz-Wieczorska A., Kot K., Olesiński I.: Immunohistochemical evaluation of caspase 3 expression in rats' hepatocytes after L-arginine therapy. Bull. Vet. Inst. Pulawy 2010, 54, 101-103.
  • 14.Rytlewski K., Zdebski Z.: Nitric oxide - one of the mechanisms that prevent changes that occur during pregnancy. Gin. Pol. 2001, 72, 738-743.
  • 15.Sulejczak D.: Apoptosis and methods of identification of this phenomenon. Post. Biol. Kom. 2000, 27, 527-568.
  • 16.Wolter K. G., Hsu Y. T., Smith C. L., Nechushtan A., Xi X. G., Youle R. J.: Movement of BAX from the cytosol to mitochondria during apoptosis. J. Cell Biol. 1997, 139, 1281-1292.
  • 17.Yigong S.: Apoptosome. Structure 2002, 10, 285-288.

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Bibliografia

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