Immunogold method evidences that kinesin and myosin bind to and couple microtubules and actin filaments in lipotubuloids of Ornithogalum umbellatum ovary epidermis

• Autorzy: Kwiatkowska M. , Polit J. T. , Poplonska K. , Stepinski D. , Wojtczak A.
• Języki publikacji: EN

Abstrakty

EN

Lipotubuloids in ovary epidermis of Ornithogalum umbellatum which are a domain of cytoplasm containing a lot of lipid bodies, microtubules and actin filaments, ribosomes, endoplasmic reticulum as well as scarce mitochondria, microbodies, dictyosomes, autolytic vacuoles, exhibit progressive-rotary motion. The immunogold method demonstrated that microtubules and actin filaments of lipotubuloids might be connected with one another by myosin and kinesin. It was supposed that collaboration of motor proteins with actin filaments and microtubules makes autonomic high peripheral speed rotary motion of lipotubuloids in epidermis cells possible. Moreover, myosin was also detected in Golgi bodies in lipotubuloid. In lipotubuloids, the immunogold method demonstrated immunosignals after the use of an antibody to dynein light chains but spectroscopy mass analysis showed that in O. umbellatum epidermis lacked dynein heavy chains.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2013
• Tom: 35
• Numer: 06
• Opis fizyczny: p.1967-1977,fig.,ref.

Twórcy

autor

Kwiatkowska M.

• Department of Cytophysiology, Faculty of Biology and Environmental Protection, University of Ło´dz´, Pomorska 141/143, 90-236 Ło´dz´, Poland

autor

Polit J. T.

• Department of Cytophysiology, Faculty of Biology and Environmental Protection, University of Ło´dz´, Pomorska 141/143, 90-236 Ło´dz´, Poland

autor

Poplonska K.

• Department of Cytophysiology, Faculty of Biology and Environmental Protection, University of Ło´dz´, Pomorska 141/143, 90-236 Ło´dz´, Poland

autor

Stepinski D.

• Department of Cytophysiology, Faculty of Biology and Environmental Protection, University of Ło´dz´, Pomorska 141/143, 90-236 Ło´dz´, Poland

autor

Wojtczak A.

• Department of Cytophysiology, Faculty of Biology and Environmental Protection, University of Ło´dz´, Pomorska 141/143, 90-236 Ło´dz´, Poland

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