Effects of Neuromedin S on the proliferation of splenic lymphocytes and the cytokine secretion by pulmonary alveolar macrophages in pigs in vitro

• Autorzy: Lin R. , Wang Q. , Qi B. , Huang Y. , Yang G.
• Języki publikacji: EN

Abstrakty

EN

Neuromedin S (NMS), a 36-amino acid neuropeptide, has been found to be involved in the regulation of the endocrine activity. It has been also detected in immune tissues in mammals, what suggests that NMS may play an important role in the regulation of immune response. The aim of this study was to demonstrate the presence of NMS receptor 1 (NMU1R) and effect of NMS in pig splenic lymphocytes (SPLs) and pulmonary alveolar macrophages (PAMs). The presence of NMU1R in pig SPLs and PAMs was respectively confirmed by reverse transcription-polymerase chain reaction (RT-PCR), western blot analysis and immunocytochemical methods. Furthermore, SPL proliferation was analyzed using the 3-(4,5)-dimethyl-thiahiazo-(-2-yl)-3,5-di-phenytetrazoliumromide (MTT) method. Additionally, the secretion of interleukin (IL)-1β, IL-6 and tumor necrosis factor-α (TNF-α) in PAMs was all measured by enzyme-linked immunosorbent assay (ELISA) kits. In the present study, the results of RT-PCR and western blot analysis revealed that NMU1R mRNA and protein were both expressed in pig SPLs and PAMs, and the immunocytochemical investigations further revealed that the positive signal of NMU1R immunoreactivity was observed in plasma membranes of both SPLs and PAMs. In the in vitro study, we found that at concentrations of 0.001-1000 nM NMS alone or combined with lipopolysaccharide or phytohemagglutinin significantly increased SPL proliferation. Application of ELISA method showed that NMS could induce the secretion of the pro-inflammatory cytokines IL-1β, IL-6 and TNF-α in PAMs. These results suggest that NMS can act as a potently positive pro-inflammatory factor and immunomodulatory agent that affects the immune response of immune cells by combining with its receptor NMU1R.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2016
• Tom: 19
• Numer: 3
• Opis fizyczny: p.485-494,fig.,ref.

Twórcy

autor

Lin R.

• University Key Laboratory for Integrated Chinese Traditional and Western Veterinary Medicine and Animal Healthcare, College of Animal Science, Fujian Agriculture and Forestry University, 15th Shang-xia-dian Road, Fuzhou, Fujian Province 350002, PR China

autor

Wang Q.

• University Key Laboratory for Integrated Chinese Traditional and Western Veterinary Medicine and Animal Healthcare, College of Animal Science, Fujian Agriculture and Forestry University, 15th Shang-xia-dian Road, Fuzhou, Fujian Province 350002, PR China

autor

Qi B.

• University Key Laboratory for Integrated Chinese Traditional and Western Veterinary Medicine and Animal Healthcare, College of Animal Science, Fujian Agriculture and Forestry University, 15th Shang-xia-dian Road, Fuzhou, Fujian Province 350002, PR China

autor

Huang Y.

• University Key Laboratory for Integrated Chinese Traditional and Western Veterinary Medicine and Animal Healthcare, College of Animal Science, Fujian Agriculture and Forestry University, 15th Shang-xia-dian Road, Fuzhou, Fujian Province 350002, PR China

autor

Yang G.

• University Key Laboratory for Integrated Chinese Traditional and Western Veterinary Medicine and Animal Healthcare, College of Animal Science, Fujian Agriculture and Forestry University, 15th Shang-xia-dian Road, Fuzhou, Fujian Province 350002, PR China

Bibliografia

• Atsuchi K, Asakawa A, Ushikai M, Ataka K, Tanaka R, Kato I, Fujimiya M, Inui A (2010) Centrally Administered Neuromedin S Inhibits Feeding Behavior and Gastroduodenal Motility in Mice. Horm Metab Res 42(7): 535-538.
• Fujii R, Hosoya M, Fukusumi S, Kawamata Y, Habata Y, Hinuma S, Onda H, Nishimura O, Fujino M (2000) Identification of neuromedin U as the cognate ligand of the orphan G protein-coupled receptor FM-3. J Biol Chem 275(28): 21068-21074.
• Hanada R, Nakazato M, Murakami N, Sakihara S, Yoshimatsu H, Toshinai K, Hanada T, Suda T, Kangawa K, Matsukura S, Sakata T (2001) A role for neuromedin U in stress response. Biochem Biophys Res Commun 289(1): 225-228.
• Hanada T, Date Y, Shimbara T, Sakihara S, Murakami N, Hayashi Y, Kanai Y, Suda T, Kangawa K, Nakazato M (2003) Central actions of neuromedin U via corticotropin-releasing hormone. Biochem Biophys Res Commun 311: 954-958.
• Hedrick JA, Morse K, Shan L, Qiao X, Pang L, Wang S, Laz T, Gustafson EL, Bayne M, Monsma FJ Jr (2000) Identification of a human gastrointestinal tract and immune system receptor for the peptide neuromedin U. Mol Pharmacol 58: 870-875.
• Howard AD, Wang R, Pong S, Mellin TN, Strack A, Guan XM, Zeng Z, Williams Jr, David L, Feighner SD, Nunes CN, Murphy B, Stair JN, Yu H, Jiang Q, Clements MK, Tan CP, McKee KK, Hreniuk DL, McDonald TP, Lynch KR, Evans JF, Austin CP, Caskey CT, Van der Ploeg L H, Liu Q (2000) Identification of receptors for neuromedin U and its role in feeding. Nature 406(6791): 70-74.
• Ida T, Mori K, Miyazato M, Egi Y, Abe S, Nakahara K, Nishihara M, Kangawa K, Murakami N (2005) Neuromedin S is a novel anorexigenic hormone. Endocrinology 146(10): 4217-4223.
• Johnson EN, Appelbaum ER, Carptenter DC, Cox RF, Disa J, Foley JJ, Ghosh SK, Naselsky DP, Pullen MA, Sarau HM, Scheff SR, Steplewski KM, Zaks-Zilberman M, Aiyar N (2004) Neuromedin Elicits Cytokine Release in Murine Th2-Type T Cell Clone D10.G4.1. J Immunol 173(11): 7230-7238.
• Kelley J (1990) Cytokines of the lung. Am Rev Respir Dis 141(3): 765-788.
• Malendowicz LK, Ziolkowska A, Rucinski M (2012) Neuromedins U and S involvement in the regulation of the hypothalamo-pituitary-adrenal axis. Front Endocrinology 3: 156.
• Mori K, Miyazato M, Ida T, Murakami N, Serino R, Ueta Y, Kojima M, Kangawa K (2005) Identification of neuromedin S and its possible role in the mammalian circadian oscillator system. EMBO J 24(2): 325-335.
• Mori K, Miyazato M, Kangawa K (2008) Neuromedin S: discovery and functions. Results Probl Cell Differ 46: 201-212.
• Moriyama M, Sato T, Inoue H, Fukuyama S, Teranishi H, Kangawa K, Kano T, Yoshimura A, Kojima M (2005) The neuropeptide neuromedin U promotes inflammation by direct activation of mast cells. J Exp Med 202(2): 217-224.
• Moriyama M, Matsukawa A, Kudoh S, Takahashi T, Sato T, Kano T, Yoshimura A, Kojima M (2006a) The neuropeptide neuromedin U promotes IL-6 production from macrophages and endotoxin shock. Biochem Biophys Res Commun 341(4): 1149-1154.
• Moriyama M, Fukuyama S, Inoue H, Matsumoto T, Sato T, Tanaka K, Kinjyo I, Kano T, Yoshimura A, Kojima M (2006b) The neuropeptide neuromedin U activates eosinophils and is involved in allergen-induced eosinophilia. Am J Physiol Lung Cell Mol Physiol 290(5): L971-L977.
• Nakazato M, Murakami N, Date Y, Mondal MS, Kojima MY, Kangawa K, Matsukura S, Hanada R (2000) Central effects of neuromedin U in the regulation of energy homeostasis. Biochem Biophys Res Commun 277(1): 191-194.
• Nakahara K, Kojima M, Hanada R, Egi Y, Ida T, Miyazato M, Kangawa K, Murakami N (2004) Neuromedin U is involved in nociceptive reflexes and adaptation to environmental stimuli in mice. Biochem Biophys Res Commun 323(2): 615-620.
• Peier A, Kosinski J, Coxyork K, Qian Y, Desai K, Feng Y, Trivedi P, Hastings N, Marsh D J (2009) The Antiobesity Effects of Centrally Administered Neuromedin U and Neuromedin S Are Mediated Predominantly by the Neuromedin U Receptor 2 (NMUR2). Endocrinology 150(7): 3101-3109.
• Sakamoto T, Mori K, Nakahara K, Miyazato M, Kangawa K, Sameshima H, Murakami N (2007) Neuromedin S exerts an antidiuretic action in rats. Biochem Biophysi Res Commun 361(2): 457-461.
• Shousha S, Nakahara K, Sato M, Mori K, Miyazato M, Kangawa K, Murakami N (2006) Effect of neuromedin S on feeding regulation in the Japanese quail. Neurosci Lett 391(3): 87-90.
• Solis-Maldonado C, Quintanilla-Licea R, Tamez-Guerra R, Rodrtguez-Padilla C, Gomez- Flores R (2003) Differential effects of synthetic indoloquinolizines on in vitro rat lymphocyte and macrophage functions. Int Immunopharmacol 3(9): 1261-1271.
• Vigo E, Roa J, López M, Castellano JM, Fernandez-Fernandez R, Navarro VM, Pineda R, Aguilar E, Dieguez C, Pinilla L, Tena-Sempere M (2007) Neuromedin S as novel putative regulator of luteinizing hormone secretion. Endocrinology 148(2): 813-823.
• Wang F, Zhang Y, Jiang X, Zhang Y, Zhang L, Gong S, Liu C, Zhou L, Tao J (2011) Neuromedin U inhibits T-type Ca2+ channel currents and decreases membrane excitability in small dorsal root ganglia neurons in mice. Cell Calcium 49(1): 12-22.
• Yang G, Su J, Li X, Yao Y, Lei Z, Yang X, Kou R, Liu Y (2009) Expression of NMS and NMU2R in the pig reproductive axis during the estrus cycle and the effect of NMS on the reproductive axis in vitro. Peptides 30(12): 2206-2212.
• Yang G, Su J, Yao Y, Lei Z, Zhang G, Li X (2010) The regulatory mechanism of neuromedin S on luteinizing hormone in pigs. Anim Reprod Sci 122(3-4): 367-374.
• Yang G, Su J, Yao Y, Lei Z, Zhang G, Liu Y, Liu J, Li X (2012) Distribution of neuromedin S and its receptor NMU2R in pigs. Res Vet Sci 92(2): 180-186.
• Yao Y, Su J, Yang G, Zhang G, Lei Z, Zhang F, Li X, Kou R, Liu Y, Liu J (2011) Effects of neuropeptide S on the proliferation of splenic lymphocytes, phagocytosis, and proinflammatory cytokine production of pulmonary alveolar macrophages in the pig. Peptides 32(1): 118-124.

Identyfikatory

DOI

10.1515/pjvs-2016-0061