Effects of lead on cholinergic SN56 neuroblastoma cells

• Autorzy: Jankowska-Kulawy A. , Gul-Hinc S. , Bielarczyk H. , Suszkiw J. B. , Pawelczyk T. , Dys A. , Szutowicz A.
• Języki publikacji: EN

Abstrakty

EN

Age-dependent accumulation of lead in brain has been implicated in the pathomechanisms of Alzheimer's disease. The aim of this work was to investigate whether cholinotoxic effects of lead may result from alterations in acetyl-CoA metabolism. One day exposure of differentiated SN56 cholinergic neuroblastoma cells to 0.5 micromol/L lead or 0.01 mmol/L amyloid-beta1-42, increased fraction of nonviable cells to about 20%. Suppression of choline acetyltransferase activity occurred only in the presence of fresh amyloid-beta1-42, whereas lead was ineffective. All agents in combination caused suppression of acetyl-CoA in cytoplasm and mitochondria down to 19% and 34% of controls, respectively. Inverse correlation was observed between whole cell acetyl-CoA level and fraction of nonviable cells at different combinations of lead and other neurotoxic compounds. It indicates that lead had no primary suppressive effect on cholinergic phenotype but, at least in part, exerted cytotoxic influence on cholinergic neurons through the decrease of their acetyl-CoA.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2008
• Tom: 68
• Numer: 4
• Opis fizyczny: p.453-462,fig.,ref.

Twórcy

autor

Jankowska-Kulawy A.

• Chair of Clinical Biochemistry, Department of Laboratory Medicine, Medical University of Gdansk, Gdansk, Poland

autor

Gul-Hinc S.

• Chair of Clinical Biochemistry, Department of Laboratory Medicine, Medical University of Gdansk, Gdansk, Poland

autor

Bielarczyk H.

• Chair of Clinical Biochemistry, Department of Laboratory Medicine, Medical University of Gdansk, Gdansk, Poland

autor

Suszkiw J. B.

• Chair of Clinical Biochemistry, Department of Molecular Medicine, Medical University of Gdansk, Gdansk, Poland

autor

Pawelczyk T.

• Department of Physiology and Biophysics, University of Cincinnati College of Medicine, Cincinnati, Ohio, USA

autor

Dys A.

• Chair of Clinical Biochemistry, Department of Laboratory Medicine, Medical University of Gdansk, Gdansk, Poland

autor

Szutowicz A.

• Chair of Clinical Biochemistry, Department of Laboratory Medicine, Medical University of Gdansk, Gdansk, Poland

Uwagi

PL

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