Striking differences between mesenchymal stem cells derived from neonatal (Wharton jelly) and adult (bone marrow) human tissues

• Autorzy: Drela K. , Siedlecka P. , Wielgos M. , Sarnowska A. , Lukomska B. , Domanska-Janik K.
• Języki publikacji: EN

Abstrakty

EN

Mesenchymal stem cells (MSC) exert unique ability to differentiate into various cells of mesodermal origin. These properties place MSC as a very promising source of cells for regenerative medicine and tissue engineering. Recently, it has been shown that experimental transplantation of MSC improves a variety of neurological dysfunctions. Bone marrow (BM) represents the mostly exploited source of human therapeutic stem cells but similar populations have been recently identified in many other tissues and organs. Among them umbilical cord Wharton jelly (WJ) has been recognized for its safety, accessibility and differentiation potential. This study compares human Wharton jelly-derived MSC (WJ-MSC) and human bone marrow-derived MSC (BM-MSC) in terms of cell phenotype, optimal growth and multilineage differentiation characteristics with special attention to neurogenic potential demonstrated by both type of cells. Materials and Methods: MSC were isolated from human Wharton jelly and human bone marrow then cultured in vitro in Lonza medium in defined conditions. Then both cell types were subjected to the specific induction media (Gibco) to analyze their potential to differentiate into osteo-, chondro- adipo- and myogenic lineages. Transcriptional activity of genes characteristic for early and late stages of cell differentiation has been examined using RT-PCR. Concomitantly immunochemical analysis of certain gene-related proteins has been performed by immunocytochemical methods. Results: We have demonstrated that both isolated WJ-MSC and BM-MSC exhibited characteristic, mesenchymal cell specific phenotypes by expressing the panels of surface antigens (CD73, CD90, CD105, CD166) as well as typical for MSC multilineage differentiation markers. However, efficiency of these processes differs markedly between the cells derived from each of examined tissues. Thus, WJ-MSC appeared to be much less prone to adipogenic differentiation in comparison to BM-MSC. In contrast, WJMSC revealed higher proliferation and neural differentiations potential than BM-MSC. Consistently, only WJ-MSC-derived cells unveiled neural progenitor characteristics expressing panel of cellular markers typical for neural lineage differentiation, i.e. Nestin, NF200, GFAP. All together, these data allow us to hypothesize that the fetal origin of WJ tissue determines its distinguished neuro-mesenchymal characteristic. This is consisted with the data of Takashima et al. (2007) showing that neonatal MSC cultures contain substantially high number of cells being descendants of the earliest wave of developmentally discern neuroepithelial MSC lineage derived from cranial part of neural crest and clearly partitioned from MSC residing in adult bon marrow niche. Conclusions: The study demonstrated that WJ-MSC, similarly to BM-MSC, can be effectively expanded in culture up to 6–8 passages when maintaining cells in undifferentiated state expressing common MSC markers. In contrast, the both MSC lines differ markedly in their ability to lineage differentiation. The most striking difference was that only WJ-MSC can be induced to neural phenotypes. Consisted with this observation WJMSC seems to be more favorable than BM-MSC to cell replacement therapy of neurodegenerative diseases. Supported by NSC grant No 2011/01/B/NZ3/0540

Słowa kluczowe

EN

differentiation; mesenchymal stem cell; neonatal period; Wharton's jelly; adult; bone marrow; human tissue; tissue; stem cell; phenotype; neurodegenerative disease

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2013
• Tom: 73
• Numer: 1
• Opis fizyczny: p.185-186

Twórcy

autor

Drela K.

• NeuroRepair Department, Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland

autor

Siedlecka P.

• NeuroRepair Department, Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland

autor

Wielgos M.

• Department of Obstetrics and Gynecology, Medical University of Warsaw, Warsaw, Poland

autor

Sarnowska A.

• NeuroRepair Department, Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland

autor

Lukomska B.

• NeuroRepair Department, Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland

autor

Domanska-Janik K.

• NeuroRepair Department, Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland