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2012 | 30 | 1 |

Tytuł artykułu

The chemokine receptor 1 gene polymorphism and its association with Somatic Cell Score and milk production traits in dairy cattle

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CXC chemokine receptors 1 (CXCR1) play a key role in inflammatory response and eventual activation of the innate immunity. Mastitis is a big problem in dairy industry. In the presented study the bovine CXCR1 gene was considered a candidate gene of anti-mastitis defense in Chinese Holsteins.Polymorphism of bovine CXCR1 was investigated and its associations with Somatic Cell Scores (SCS) and milk production traits (milk yield, milk fat and milk protein content) analysed. Six single nucleotide polymorphisms (SNP) were identified within a 311 bp segment of the bovine CXCR1 locus at positions +570 (G>A), +642 (G>A), +735 (G>C), +816 (C>A), +819 (A>G) and +820 (G>A) relative to the available mRNA sequence, of which CXCR1c.+820 G>A was revealed for the first time. The SNP CXCR1c.+735G>C resulted in the mutation of amino acid, but it showed no significant association with the SCS, milk yield, milk fat and milk protein percentage of Chinese Holsteins (P>0.05). Fifteen haplotypes were reconstructed by the six SNPs in the sample of Chinese Holstein population. The most common haplotype was Haplo1 carrying alleles AAGCAG equal to 0.37, followed by 7 haplotypes (from 2 to 7) with frequencies equal to 0.13, 0.11, 0.08, 0.08, 0.07, 0.07 and 0.06, respectively. Haplotype analysis revealed no significant associations with SCS and other milk production traits.

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  • Istitute of Cellular and Molecular Biology, Xuzhou Normal University, Xuzhou, Jiangsu, China


  • Ahuja S.K., Murphy P.M., Tiffany H.L., 1996 – The CXC chemokines growth-regulated oncogene (GRO)-alpha, -beta, -gamma, neutrophil activating peptide-2, and epithelial cell-derived neutrophil activating peptide-78 are potent agonists for the type B, but not the type A, human interleukin-8 receptor. Journal of Biological Chemistry 271, 20545-20550.
  • Ali K.A., Shook G..E., 1980 – An optimum transformation for somatic cell concentration in milk.Journal of Dairy Science 63, 487-490.
  • Burvenich C., Paape M.J., Hill A.E., Guidry A.J., 1994 – Role of the neutrophil leucocyte in the local and systemic reactions during experimentally induced E. coli mastitis in cows immediately after calving. Veterinary Quarterly 16, 45-50.
  • Fetrow J., 2000 – Mastitis: An Economic Consideration. pp. 3-47, National Mastitis Council, Verona, WI.
  • Grosse W.M., Kappes S.M., Laegreid W.W., Keele J.W., 1999 – Single nucleotide polymorphism (snp) discovery and linkage mapping of bovine cytokine genes. Mammalian Genome 10, 1062-1069.
  • Hill A.W., 1981 – Factors influencing the outcome of Escherichia coli mastitis in the dairy cow. Research in Veterinary Science 31, 107-112.
  • Lahouassa H., Rainard P., Caraty A., Riollet C., 2008 – Identification and characterization of a new interleukin-8 receptor in bovine species. Molecular Immunology 45, 1153-1164.
  • Leyva-Baca I., Schenkel F., Sharma B.S., Jansen G.B., 2007– Identification of single nucleotide polymorphisms in the bovine CCL2, IL8, CCR2 and IL8RA genes and their association with health and production in Canadian Holsteins. Animal Genetics 38, 198-202.
  • Leyva-Baca I., Schenkel F., Martin J., Karrow N.A., 2008a – Polymorphisms in the 5’upstream region of the CXCR1 chemokine receptor gene, and their association with Somatic Cell Score in Holstein cattle in Canada. Journal of Dairy Science 91, 407-417.
  • Leyva-Baca I., Pighetti G., Karrow N.A., 2008b – Genotype-specific IL8RA gene expression in bovine neutrophils in response to Escherichia coli lipopolysaccharide challenge. Animal Genetics 39, 298-300.
  • Mitchell G.B., Albright B.N., Casewell J.L., 2003 – Effect of interleukin-8 and granulocyte colony stimulating factor on priming and activation of bovine neutrophils. Infection and Immunity 71, 1643-1649.
  • Pighetti G.M., Rambeaud M., 2006 – Genome conservation between the bovine and human interleukin-8 receptor complex: Improper annotation of bovine interleukin-8 receptor b identified.Veterinary Immunology and Immunopathology 114, 335-340.
  • Peveri P., Walz A., Dewald B., Baggiolini M., 1988 – A novel neutrophil-activating factor produced by human polymorphonuclear leukocytes. Journal of Experimental Medicine 181, 1547-1559.
  • Rambeaud M., Pighetti G.M., 2005 – Impaired neutrophil migration associated with specific bovine CXCR2 genotypes. Infection and Immunity 73, 4955-4959.
  • SAS Institute , 1999 – SAS User’s Guide. SAS OnlineDoc, Version 8. SAS Inst., Inc., Cary,NC.
  • Sambrook J, Russell D.W., 2001 – Molecular Cloning (3rd ed.). pp. 636-648. Cold Spring Harbor Laboratory Press, New York.
  • Youngerman S.M., Saxton A.M., Oli ver S.P., Pighetti G..M., 2004a – Association of CXCR2 polymorphisms with subclinical and clinical mastitis in dairy cattle. Journal of Dairy Science 87, 2442-2448.
  • Youngerman S.M., Saxton A.M., Pighetti G.M., 2004b – Novel single nucleotide polymorphisms and haplotypes within the bovine CXCR2 gene. Immunogenetics 56, 355-359.
  • Zhang C.L., Wang Y., Chen H., Lan XY., 2007 – Enhance the efficiency of single-strand conformation polymorphism analysis by short polyacrylamide gel and modified silver staining.Analytical Biochemistry 365, 286-287.


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