Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2012 | 15 | 3 |

Tytuł artykułu

Effect of conglutinin on phagocytic activity of bovine granulocytes

Treść / Zawartość

Warianty tytułu

Języki publikacji



In the present study we investigated the effect of bovine conglutinin on the phagocytic activity of leukocytes. We measured both the chemotactic activity of conglutinin and its effect on the internalization of zymosan particles and E. coli by granulocytes. We also assessed the binding of conglutinin to various microorganisms isolated from clinical cases in cattle. We showed that conglutinin binds strongly to the surface of yeast cells and to mannan-rich zymosan particles, while weak binding was observed in the case of the bacterial strains tested, including those whose O antigen is composed of mannan. Conglutinin (1-10 μg/ml) neither acts as a chemotactic factor for peripheral blood leukocytes nor affects ingestion of E. coli by granulocytes. However, as flow cytometry based assay showed, conglutinin (0.1-1 μg/ml) increased ingestion of zymosan expressed as mean fluorescence intensity (MFI) of positive cells.








Opis fizyczny



  • Institute of Biological Bases of Animal Diseases, Subdepartment of Veterinary Prevention, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Akademicka 12, 20-033 Lublin, Poland


  • Brouwer N, Dolman KM, van Houdt M, Sta M, Roos D, Kuijpers TW (2008) Mannose-binding lectin (MBL) facilitates opsonophagocytosis of yeasts but not of bacteria despite MBL binding. J Immunol 180: 4124-4132.
  • Dec M, Wernicki A (2006) Conglutinin, Cl-43 and Cl-46 – three bovine collectins. Pol J Vet Sci 9: 265-275.
  • Dec M, Wernicki A, Puchalski A, Urban-Chmiel R (2011a) Conglutinin is not specific to cattle. Veterinarni Medicina 56: 510-519.
  • Dec M, Wernicki A, Puchalski A, Urban-Chmiel R, Graz M (2012b) Purification and electrophoretic characterization of bovine conglutinin. Biomed Chromatogr 26: 684-690.
  • Dec M, Wernicki A, Puchalski A, Urban-Chmiel R, Waśko A (2012a) The effect of conglutinin on production of reactive oxygen species in bovine granulocytes. J Vet Sci 13: 33-38.
  • Friis-Christiansen P, Thiel S, Svehag SE, Dessau R, Svendsen P, Andersen O, Laursen SB, Jensenius JC (1990) In vivo and in vitro antibacterial activity of conglutinin, a mammalian plasma lectin. Scand J Immunol 31: 453-460.
  • Friis P, Svehag SE, Andersen O, Gahrn-Hansen B, Leslie RG (1991) Conglutinin exhibits a complement-dependent enhancement of the respiratory burst of phagocytes stimulated by E. coli. Immunology 74: 680-684.
  • Hartshorn KL, Sastry K, Brown D, White MR, Okarma TB, Lee YM, Tauber AI (1993) Conglutinin acts as an opsonin for influenza A viruses. J Immunol 151: 6265-6273.
  • Hartshorn KL, White MR, Crouch EC (2002) Contributions of the N- and C-terminal domains of surfactant protein d to the binding, aggregation, and phagocytic uptake of bacteria. Infect Immun 70: 6129-6139.
  • Hogenkamp A, van Eijk M, van Dijk A, van Asten AJ, Veldhuizen EJ, Haagsman HP (2006) Characterization and expression sites of newly identified chicken collectins. Mol Immunol 43: 1604-1616.
  • Holmskov U, Teisner B, Pedersen NT, Laursen SB, Rasmussen HB, Jensenius JC (1992) Tissue localization of conglutinin, a bovine C-type lectin. Immunology 76: 169-173.
  • Ingram DG (1959) The conglutination phenomenon. XIV. The resistance enhancing effect of conglutinin and immuno-conglutinin in experimental bacterial infections. Immunology 2: 334-345.
  • Ingram DG, Mitchell WR (1971) Conglutinin level in dairy cattle: changes associated with disease. Am J Vet Res 32: 875-878.
  • Katsuda K, Kamiyama M, Kohmoto M, Kawashima K, Tsunemitsu H, Eguchi M (2008) Serotyping of Mannheimia haemolytica isolates from bovine pneumonia: 1987-2006. Vet J 178: 146-148.
  • Kobayashi H, Oyamada H, Matsuda K, Shibata N, Suzuki S (2003) Distribution of antigenic oligomannosyl side chains in the cell wall mannans of several strains of Candida tropicalis. Arch Microbiol 180: 76-80.
  • Krogh-Meibom T, Ingvartsen KL, Tornoe I, Palaniyar N, Willis AC, Holmskov U (2010) A simple two-step purification procedure for the iC3b binding collectin conglutinin. J Immunol Methods 31: 204-208.
  • Madan T, Eggleton P, Kishore U, Strong P, Aggrawal SS, Sarma PU, Reid KB (1997) Binding of pulmonary surfactant proteins A and D to Aspergillus fumigatus conidia enhances phagocytosis and killing by human neutrophils and alveolar macrophages. Infect Immun 65: 3171-3179.
  • Nair SV, Pearce S, Green PL, Mahajan D, Newton RA, Raftos DA (2000) A collectin-like protein from tunicates. Comp Biochem Physiol B Biochem Mol Biol 125: 279-289.
  • Nakao M, Kajiya T, Sato Y, Somamoto T, Kato-Unoki Y, Matsushita M, Nakata M, Fujita T, Yano T (2006) Lectin pathway of bony fish complement: identification of two homologs of the mannose-binding lectin associated with MASP2 in the common carp (Cyprinus carpio). J Immunol 177: 5471-5479.
  • Ovodov YS (2006) Bacterial capsular antigens. Structural patterns of capsular antigens. Biochemistry (Mosc) 71: 937-954.
  • Reading PC, Holmskov U, Anders EM (1998) Antiviral activity of bovine collectins against rotaviruses. J Gen Virol 79: 2255-2263.
  • Schagat TL, Wofford JA, Greene KE, Wright JR (2003) Surfactant protein A differentially regulates peripheral and inflammatory neutrophil chemotaxis. Am J Physiol Lung Cell Mol Physiol 284: 140-147.
  • Śliwa-Dominiak J, Tokarz-Deptuła B, Niedźwiedzka-Rystwej P, Deptuła W (2010) Conglutinin – an Important Element of Natural Immunity in Ruminants. Acta Vet Brno 79: 99-104.
  • Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76: 4350-4354.
  • Urban-Chmiel R, Puchalski A, Wernicki A, Dec M, Paluch E (2009a). Characterization of Hsp70 proteins in bovine leukocytes induced by the temperature 41 degrees C. Pol J Vet Sci 12: 323-328.
  • Urban-Chmiel R, Wernicki A, Puchalski A, Dec M (2009b). In vitro effect of h-tocopherol and ascorbic acid supplementation on immunological indicators in bovine leukocytes following transportation. Acta Vet Brno 78: 589-594.
  • Van de Wetering JK, van Golde LM, Batenburg JJ (2004) Collectins: players of the innate immune system. Eur J Biochem 271: 1229-1249.
  • Von Bredow C. Hartl D, Schmid K, Schabaz F, Brack E, Reinhardt D, Griese M (2006) Surfactant protein D regulates chemotaxis and degranulation of human eosinophils. Clin Exp Allergy 36: 1566-1574

Typ dokumentu



Identyfikator YADDA

JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.