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2014 | 63 | 3 |
Tytuł artykułu

Application of microsatellite-primed PCR (MSP-PCR) and PCR melting profile (PCR-MP) method for intraspecies differentiation of dermatophytes

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
In this study, two PCR-based methods (MSP-PCR and PCR-MP) were compared for their abilities to identify intraspecies variations of 23 isolates of Trichophyton rubrum, 78 isolates of Trichophyton interdigitale and 22 isolates of Microsporum canis, obtained mainly from patients in Łódź city. The results allowed to distinguish four types (containing two subtypes) characteristic for T. interdigitale and three types characteristic for T. rubrum using PCR-MP method. Analysis conducted using MSP-PCR with (GACA)4 primer revealed four types for T. rubrum and three types (containing one subtype) for T. interdigitale and with (GTG)5 primer showed two types (containing one subtype) for T. rubrum and six types (containing one subtype) for T. interdigitale. No differentiation was observed for the M. canis isolates with either method.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
63
Numer
3
Opis fizyczny
p.283-290,fig.,ref.
Twórcy
  • Department of Microbial Genetics, University of Lodz, Lodz, Poland
autor
  • The 2nd Department of Dermatology, Pediatric Dermatology and Oncology Clinic, Medical University of Lodz, Lodz, Poland
autor
  • Department of Microbial Genetics, University of Lodz, Lodz, Poland
autor
  • Department of Genetics, Institute of Applied Biotechnology and Basic Sciences, University of Rzeszow, Poland
autor
  • Department of Microbial Genetics, University of Lodz, Lodz, Poland
  • Academy of Social Sciences, Lodz, Poland
autor
  • Department of Microbial Genetics, University of Lodz, Lodz, Poland
Bibliografia
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  • Dobrowolska A., P. Stączek and M. Kozłowska. 2006. PCR-RFLP analysis of the dermatophytes isolated from patients in Central Poland. J. Dermatol. Sci. 42: 71–74.
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  • Rezaei-Matehkolaei A., K. Makimura, and S. Nouripour-Sisakht. 2012. Use of single-enzyme PCR-restriction digestion barcode targeting the internal transcribed spacers (ITS rDNA) to identify dermatophyte species. Iran J. Public Health. 41: 82–94.
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  • Shehata A.S., P.K. Mukherjee and M.A. Ghannoum. 2008. Single-step PCR using (GACA)4 primer: utility for rapid identification of dermatophyte species and strains. J. Clin. Microbiol. 46: 2641–2645.
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Typ dokumentu
Bibliografia
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Identyfikator YADDA
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