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2013 | 35 | 10 |

Tytuł artykułu

Application of exogenous substances reduces tobacco-specific nitrosamines content by regulating biosynthesis of nicotine and nitrite in burley tobacco

Warianty tytułu

Języki publikacji



Tobacco-specific nitrosamines (TSNAs) are carcinogenic chemicals found in tobacco plants. The increasing health consciousness of individuals had led to an increased interest in research on reducing TSNAs content. The aim of this study was to use a pot experiment in which exogenous substances were applied to burley tobacco to dissect the mechanism of TSNAs production. The results indicated that spraying the exogenous substances IAA, NAA, SA and combination thereof on burley tobacco after topping decreased TSNAs content by 2.69–29.4 % in upper leaves and 0.23–39.3 % in middle leaves without affecting total sugar, total nitrogen, potassium and chlorine contents. The application of exogenous substances could down-regulate expression of the NR gene and the activity of the NR enzyme, resulting in less accumulation of the TSNAs precursor nitrite. The exogenous substances significantly reduced nicotine accumulation, which was consistent with low enzyme activities and the down-regulated expressions of genes involved in nicotine biosynthesis, especially significant in the case of quinolinate phosphoribosyltransferase. These results suggested that the application of exogenous substances on burley tobacco after topping could reduce TSNAs content which may be attributed to the regulation of exogenous substances on nitrite and nicotine. This also implies one potential improvement to agronomic practices aimed at controlling the accumulation of TSNAs in burley tobacco.

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Opis fizyczny



  • Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze River), Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, China
  • Burley Tobacco Experimental Station of CNTC, Wuhan 430030, China
  • Test Center, R&D, Beijing Tobacco Factory, Shanghai Tobacco Group Co. LTD., Beijing 101121, China
  • Burley Tobacco Experimental Station of CNTC, Wuhan 430030, China
  • Test Center, R&D, Beijing Tobacco Factory, Shanghai Tobacco Group Co. LTD., Beijing 101121, China
  • Test Center, R&D, Beijing Tobacco Factory, Shanghai Tobacco Group Co. LTD., Beijing 101121, China
  • Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze River), Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, China
  • Burley Tobacco Experimental Station of CNTC, Wuhan 430030, China


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