Comparison of different molecular methods for detection of Mycoplasma synoviae

• Autorzy: Domanska-Blicharz K. , Tomczyk G. , Minta Z.
• Języki publikacji: EN

Abstrakty

EN

The "in house" PCR method and four commercial PCR kits (designed symbolically as A, B, C, and D) for the detection of Mycoplasma synoviae (MS) in tracheal swabs of infected experimentally SPF chickens were compared. The chickens were inoculated intranasally with 1x10⁴ colour change unit (cfu)/mL of ATCC reference MS strain, and at 4, 7, 14, 28, and 35 d post infection (d.p.i.), the tracheal samples were collected and examined. The methods showed different sensitivities. The commercial test C seemed to be less sensitive (0.5 ng/mL) than the rest of the methods (50 pg/mL). Only the "in house" method and commercial test D detected MS DNA from 7 to 14 d.p.i.; no satisfactory results were obtained with the other kits. Since the methods have shown different sensitivity, their suitability for MS detection seems to be limited.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2009
• Tom: 53
• Numer: 3
• Opis fizyczny: p.357-360,fig.,ref.

Twórcy

autor

Domanska-Blicharz K.

• Department of Poultry Diseases, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Tomczyk G.

• Department of Poultry Diseases, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Minta Z.

• Department of Poultry Diseases, National Veterinary Research Institute, 24-100 Pulawy, Poland

Bibliografia

• 1. Abdul-Wahab O.M., Ross G., Bradbury J.M.: Pathogenicity and cytadherence of Mycoplasma imitans in chicken and duck embryo tracheal organ cultures. Infect Immun 1996, 64, 563-568.
• 2. Barry T., Colleran G., Glennon M., Dunican L.K., Gannon F.: The 16S/23S ribosomal spacer region as a target for DNA probes to identify eubacteria. PCR Meth Appl 1991, 1, 51-56.
• 3. Bencina D., Drobnic-Valic M., Horvat S., Narat M., Kleven S.H., Dovc P.: Molecular basis of the length variation in the N-terminal part of Mycoplasma synoviae hemagglutinin. FEMS Microbiol Lett 2001, 203, 115-123.
• 4. Bradbury J.M.: Avian mycoplasma infections: prototype of mixed infections with mycoplasmas, bacteria and viruses. Ann Microbiol 1984, 135A, 83-89.
• 5. Ewing M.L., Cookson K.C., Phillips R.A., Turner K.R., Kleven S.H.: Experimental infection and transmissibility of Mycoplasma synoviae with delayed serologic response in chickens. Avian Dis 1998, 42, 230-238.
• 6. Ewing M.L., Lauerman L.H., Kleven S.H., Brown M.B.: Evaluation of diagnostic procedures to detect Mycoplasma synoviae in commercial multiplier-breeder farms and commercial hatcheries in Florida. Avian Dis 1996, 40, 798-806.
• 7. Feberwee A., Mekkes D.R., de Wit J.J., Hartman E.G., Pijpers A.: Comparison of culture, PCR, and different serologic tests for detection of Mycoplasma gallisepticum and Mycoplasma synoviae infections. Avian Dis 2005, 49, 260-268.
• 8. Fiorentin L., Mores M.A., Trevisol I.M., Antunes S.C., Costa L.A., Soncini R.A., Vieira N.A.: Test profiles of broiler breeder flocks housed in farms with endemic Mycoplasma synoviae infections. Brasil J Poult Sci 2003, 5, 37-43.
• 9. Harasawa R., Pitcher D.G., Ramirez A.S., Bradbury J.M.: A putative transposase gene in the 16S-23S rRNA intergenic spacer region of Mycoplasma imitans. Microbiology 2004, 150, 1023-1029.
• 10. Hong Y., Garcia M., Leiting V., Bencina D., Dufour- Zavala L., Zavala G., Kleven S.H.: Specific detection and typing of Mycoplasma synoviae strains in poultry with PCR and DNA sequence analysis targeting the hemagglutinin encoding gene vlhA. Avian Dis 2004, 48, 606-616.
• 11. Kleven S.H., Ferguson-Noel N.: Mycoplasma synoviae infections. In: Diseases of Poultry. Edited by Saif Y.M., Fadly A.M., Galisson J.R., McDougald L.R., Nolan L.K., and Swayne D.E., Blackwell Publishing, Ames, USA, 2008, pp. 845-856.
• 12. Lauerman L.H., Hoerr F.J., Sharpton A.R., Shah S.M., van Santen V.L.: Development and application of a polymerase chain reaction assay for Mycoplasma synoviae. Avian Dis 1993, 37, 829-834.
• 13. OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals. Chapter 2.3.5: Avian mycoplasmosis (Mycoplasma gallisepticum, M. synoviae), 2008, pp. 482-496.
• 14. Papazisi L., Gorton T.S., Kutish G., Markham P.F., Browning G.F., Nguyen D.K., Swartzell S., Madan A., Mahairas G., Geary S.J.: The complete genome sequence of the avian pathogen Mycoplasma gallisepticum strain R (low). Microbiology 2003, 149, 2307-2316.
• 15. Ramirez A.S., Naylor C.J., Hammond P.P., Bradbury J.M.: Development and evaluation of a diagnostic PCR for Mycoplasma synoviae using primers located in the intergenic spacer region and the 23S rRNA gene. Vet Microbiol 2006, 118, 76-82.
• 16. Razin S., Yogev D., Naot Y.: Molecular biology and pathogenesis of mycoplasmas. Microbiol Mol Biol Rev 1998, 62, 1094-1156.
• 17. Vasconcelos A.T., Ferreira H.B., Bizarro C.V., Bonatto S.L., Carvalho M.O.: Swine and poultry pathogens: the complete genome sequences of two strains of Mycoplasma hyopneumoniae and a strain of Mycoplasma synoviae. J Bacteriol 2005, 187, 5568-5577.
• 18. Weisburg W.G., Tully J.G., Rose D.L., Petzel J.P., Oyaizu H., Yang D.: A phylogenetic analysis of the mycoplasmas. Basis for their classification. J Bacteriol 1989, 12, 6455-6467.
• 19. Zain Z.M., Bradbury J.M.: Optimising the conditions for isolation of Mycoplasma gallisepticum collected on applicator swabs. Vet Microbiol 1996, 49, 45-57.