Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2013 | 35 | 04 |
Tytuł artykułu

Alleviation of salinity-induced perturbations in ionic and hormonal concentrations in spring wheat through seed preconditioning in synthetic auxins

Warianty tytułu
Języki publikacji
The experiments were conducted to examine the effects of seed priming in solutions (100, 150 and 200 mg L-1) of different synthetic auxins, i.e., 2,4- dichlorophenoxyacetic acid (2,4-D), 2,4,5-trichlorophenoxyacetic acid (2,4,5-T), α-naphthaleneacetic acid (NAA) on growth, grain yield, gaseous exchange characteristics, ionic and hormonal concentrations in two spring wheat (Triticum aestivum L.) cultivars MH-97 (salt intolerant) and Inqlab-91 (salt tolerant). The primed (soaked for 12 h) and non-primed seeds were sown in Petri plates in a growth room as well as in a field treated with 150 mM NaCl. Generally, all synthetic auxins did not increase germination percentage and rate in both cultivars when compared with hydropriming (control), and even decreased these attributes when applied at higher concentrations (200 mg L-1). Nonetheless, under salt stress, NAA (150 mg L-1) was most effective in increasing seedling shoot dry weight, fertile tillers per plant, number of grains per ear and grain yield in both cultivars. The plants raised from seed treated with NAA (150 mg L-1) had lower shoot [Na+] in the salt intolerant cultivar. Moreover, NAA treatment improved root [Ca2+] in both cultivars. Priming agents affected leaf free indole-3-acetic acid (IAA) and indole-3-butyric acid (IBA) concentrations differently in both cultivars. Treatment with NAA (150 mg L-1) lowered leaf free abscisic acid (ABA) and putrescine (Put) concentrations and raised salicylic acid (SA) and spermidine (Spd) concentrations in the salt intolerant cultivar. In conclusion, pre-treatment with NAA (150 mg L-1) showed consistent promotive effects on growth and grain yield in the two cultivars, which were partially attributed to the beneficial effects of NAA-priming on ionic and hormonal homeostasis under salt stress.
Słowa kluczowe
Opis fizyczny
  • Department of Botany, Government College University, Faisalabad 38000, Pakistan
  • Department of Botany, University of Agriculture, Faisalabad 38040, Pakistan
  • Agusti M, Gariglio N, Castillo A, Juan M, Almela V, Martinez-Fuentes A, Mesejo C (2003) Effect of the synthetic auxin 2,4-DP on fruit development of loquat. Plant Growth Regul 41:129–132
  • Azam F, Lodhi A, Farooq S, Harry-O’kuru R, Imam SH (2005) Seed treatment with phytohormones and crop productivity. III. Physiological/biochemical changes in germinating seeds and rooting characteristics of wheat (Triticum aestivum L.) following exposure to 2,4-D. Pak J Bot 37:865–874
  • Bauly J, Sealy M, Macdonald IM, Brearley H, Droge J, Hillmer S, Robinson S, Venis DG, Blatt MA, Lazarus CM, Napier RM (2000) Overexpression of auxin-binding protein enhances the sensitivity of guard cells to auxin. Plant Physiol 124:1229–1238
  • Bechtold U, Karpinski S, Mullineaux PM (2005) The influence of the light environment and photosynthesis on oxidative signalling responses in plant–biotrophic pathogen interactions. Plant Cell Environ 28:1046–1055
  • BorsaniO,Valpuesta V, BotellaMA(2001) Evidence for a role of salicylic acid in the oxidative damage generated by NaCl and osmotic stress in Arabidopsis seedlings. Plant Physiol 126:1024–1030
  • Campanoni P, Nick P (2005) Auxin-dependent cell division and cell elongation. 1-Naphthaleneacetic acid and 2,4-dichlorophenoxyacetic acid activate different pathways. Plant Physiol 137: 939–948
  • Capell T, Bassie L, Christou P (2004) Modulation of the polyamine biosynthetic pathway in transgenic rice confers tolerance to drought stress. Proc Natl Acad Sci USA 101:9909–9914
  • Chattopadhyay MK, Gupta S, Sengupta DN, Ghosh B (1997) Expression of arginine decarboxylase in seedlings of indica rice Oryza sativa L. cultivars as affected by salinity stress. Plant Mol Biol 34:477–483
  • Christiansen-Weniger C (1992) N2-fixation by ammonium-excreting Azospirillum brasilense in auxin-induced tumours of wheat (Triticum aestivum L.). Biol Fertil Soils 12:100–106
  • Cona A, Rea G, Angelini R, Federico R, Tavladoraki P (2006) Functions of amine oxidases in plant development and defence. Trends Plant Sci 11:80–88
  • Delbarre A, Mu¨ller P, Imhoff V, Guern J (1996) Comparison of mechanisms controlling uptake and accumulation of 2,4 dichlorophenoxy acetic acid, naphthalene-1-acetic acid, and indole-3-acetic acid in suspension-cultured tobacco cells. Planta 198:532–541
  • Flores HE, Galston AW (1982) Analysis of polyamines in higher plants by high performance liquid chromatography. Plant Physiol 69:701–706
  • Grieve CM, Lesch SM, Francois LE, Maas EW (1992) Analysis of main-spike yield components in salt-stressed wheat. Crop Sci 32:697–703
  • Grossman K (1990) Plant growth retardants as tools in physiological research. Physiol Plant 78:640–648
  • Gulnaz A, Iqbal J, Farooq S, Azam F (1999) Seed treatment with growth regulators and crop productivity 1. 2,4-D as an inducer of salinity-tolerance in wheat (Triticum aestivum L.). Plant Soil 210:209–217
  • Hollington PA (2000) Technological breakthroughs in screening/breeding wheat varieties for salt tolerance. In: Gupta SK, Sharma SK, Tyagi NK (eds) Proceedings national conference on salinity management in agriculture, December 1998. Central Soil Salinity Research Institute (CSSRI), Karnal, India, pp 273–289
  • Iqbal M, Ashraf M (2006) Wheat seed priming in relation to salt tolerance: growth, yield and levels of free salicylic acid and polyamines. Ann Bot Fenn 43:250–259
  • Iqbal M, Ashraf M (2007) Seed treatment with auxins modulates growth and ion partitioning in salt stressed wheat plants. J Integr Plant Biol 49:1045–1057
  • Iqbal M, Ashraf M (2010a) Changes in hormonal balance: a possible mechanism of pre-sowing chilling-induced salt tolerance in spring wheat. J Agron Crop Sci 196:440–454
  • Iqbal M, Ashraf M (2010b) Gibberellic acid mediated induction of salt tolerance in wheat plants: Growth, ionic partitioning, photosynthesis, yield and hormonal homeostasis. Environ Exp Bot (in press). doi:10.1016/j.envexpbot.2010.06.002
  • Iqbal M, Ashraf M, Jamil A (2006a) Seed enhancement with cytokinins: changes in growth and grain yield in salt stressed wheat plants. Plant Growth Regul 50:29–39
  • Iqbal M, Ashraf M, Jamil A, Rehman S (2006b) Does seed priming induce changes in the levels of some endogenous plant hormones in hexaploid wheat plants under salt stress? J Integr Plant Biol 48:181–189
  • Iqbal M, Ashraf M, Rehman S, Rha ES (2006c) Does polyamine seed pretreatment modulate growth and levels of some plant growth regulators in hexaploid wheat (Triticum aestivum L.) plants under salt stress? Bot Stud 47:239–250
  • Janda T, Horva´th E, Szalai G, Pa´ldi E (2007) Role of salicylic acid in the induction of abiotic stress tolerance. In: Hayat S, Ahmad A (eds) Salicylic acid: a plant hormone. Springer, The Netherlands, pp 91–150
  • Kasinathan V, Wingler A (2004) Effect of reduced arginine decarboxylase activity on salt tolerance and on polyamine formation during salt stress in Arabidopsis thaliana. Physiol Plant 121:101–107
  • Kiseleva IS, KaminskayaOA(2002) Hormonal regulation of assimilate utilization in barley leaves in relation to the development of their source function. Russ J Plant Physiol 49:534–540
  • Kusaba S, Kano-Murakami Y, Matsuoka M, Tamaoki M, Sakamoto T, Yamaguchi I, Fukumoto M (1998) Alteration of hormone levels in transgenic tobacco plants over expressing the rice homeobox gene OSH1. Plant Physiol 116:471–476
  • Li X, Feng Y, Boersma L (1994) Partition of photosynthates between shoot and root in spring wheat (Triticum aestivum L.) as a function of soil water potential and root temperature. Plant Soil 164:43–50
  • Liu J-H, Kitashiba H, Wang J, Ban Y, Moriguchi T (2007) Polyamines and their ability to provide environmental stress tolerance to plants. Plant Biotechnol 24:117–126
  • Loreto F, Centritto M, Chartzoulakis K (2003) Photosynthetic limitations in olive cultivars with different sensitivity to salt stress. Plant Cell Environ 26:595–601
  • Ludwig-Mu¨ller J (2011) Auxin conjugates: their role for plant development and in the evolution of land plants. J Exp Bot 62:1757–1773
  • Ludwig-Mu¨ller J, Sass S, Sutter E, Wodner M, Epstein E (1993) Indole-3-butyric acid in Arabidopsis thaliana. Plant Growth Regul 13:179–187
  • Ludwig-Mu¨ller J, Epstein E, Hilgenberg W (1996) Auxinconjugate hydrolysis in Chinese cabbage: characterization of an amidohydrolase and its role during the clubroot disease. Physiol Plant 97:627–634
  • Mansfield TA, Atkinson CJ (1990) Stomatal behaviour in waterstressed plants. In: Alscher RG, Cumming JR (eds) Stress responses in plants: adaptation and acclimation mechanisms. Wiley, New York, pp 241–264
  • Marchant A, Kargul J, May ST, Muller P, Delbarre A, Perrot-Rechenmann C, Bennett MJ (1999) AUX1 regulates root gravitropism in Arabidopsis by facilitating auxin uptake within root apical tissues. EMBO J 18:2066–2073
  • Mo H, Pua EC (2002) Up-regulation of arginine decarboxylase gene expression and accumulation of polyamines in mustard (Brassica juncea) in response to stress. Physiol Plant 114:439–449
  • Nishimura S, Maeda E (1982) Cytological studies on differentiation and dedifferentiation in pericycle cells of excised rice roots. Japan J Crop Sci 51:553–560
  • Nordstro¨m A, Tarkowsky P, Tarkowska´ D, Norbaek R, A ° stot C, Dolezal K, Sandberg G (2004) Auxin regulation of cytokinin biosynthesis in Arabidopsis thaliana: a factor of potential importance for auxin-cytokinin-regulated development. Proc Natl Acad Sci USA 101:8039–8044
  • Pe´ret B, Li G, Zhao J, Band LR, Voß U, Postaire O, Luu DT, Da Ines O, Casimiro I, Lucas M, Wells DM, Lazzerini L, Nacry P, King JR, Jensen OE, Scha¨ffner AR, Maurel C, Bennett MJ (2012) Auxin regulates aquaporin function to facilitate lateral root emergence. Nat Cell Biol (in press). doi:10.1038/ncb2573
  • Poo´r P, Ge´mes K, Szepesi A ´ , Horva´th F, Simon ML, Tari I (2011) Salicylic acid treatment via the rooting medium interferes with the stomatal response, CO2 fixation rate and carbohydrate metabolism in tomato and decreases the harmful effects of subsequent salt stress. Plant Biol 13:105–114
  • Rastogi R, Davies PJ (1991) Polyamine metabolism in ripening tomato fruit. II. Polyamine metabolism and synthesis in relation to enhanced putrescine content and storage life of alc tomato fruit. Plant Physiol 95:41–45
  • Ruuhola TM, Julkunen-Tiitto MR (2000) Salicylates of intact Salix myrsinifolia plantlets do not undergo rapid metabolic turnover. Plant Physiol 122:895–905
  • Santa-Cruz A, Perez-Alfocea MA, Bolarin C (1997) Changes in free polyamine levels induced by salt stress in leaves of cultivated and wild tomato species. Physiol Plant 101:341–346
  • Seskar M, Shulaev V, Raskin I (1998) Endogenous methyl salicylate in pathogen-inoculated tobacco plants. Plant Physiol 116:387–392
  • Shabala S, Shabala L, Volkenburgh EV (2003) Effect of calcium on root development and root ion fluxes in salinised barley seedlings. Funct Plant Biol 30:507–514
  • Shakirova FM, Sakhabudinova AR, Bezrukova MV, Fakhutdinova RA, Fakhutdinova DR (2003) Changes in the hormonal status of wheat seedlings induced by salicylic acid and salinity. Plant Sci 164:317–322
  • Sood S, Nagar PK (2004) Changes in endogenous polyamines during flower development in two diverse species of rose. Plant Growth Regul 44:117–123
  • Stern RA, Flaishman M, Applebaum S, Ben-Arie R (2007) Effect of synthetic auxins on fruit development of ‘Bing’ cherry (Prunus avium L). Sci Hort 114:275–280
  • Subbarao GV, Johansen C, Jana MK, Kao JVK (1990) Effects of sodium/calcium ratio in modifying salinity responses of pigeonpea (Cajanus cajan). J Plant Physiol 136:439–443
  • Sun Y, Yang Y, Yuan Z, Ludwig-Mu¨ller J, Yu C, Xu Y, Shao X, Li X, Decker EL, Reski R, Huang H (2010) Overexpression of the Arabidopsis gene UPRIGHT ROSETTE reveals a homeostatic control for indole-3-acetic acid. Plant Physiol 153:1311–1320
  • Timson J (1965) New method of recording germination data. Nature 207:216–217
  • Tognetti V, Van Aken O, Morreel K, Vandenbroucke K, Van De Cotte B, De Clercq I, Chiwocha S, Fenske R, Prinsen E, Boerjan W, Genty B, Stubbs K, Inze´ D, Van Breusegem F (2010) Perturbation of indole-3-butyric acid homeostasis by the UDPglucosyltransferase UGT74E2 modulates Arabidopsis architecture and water stress tolerance. Plant Cell 22:2660–2679
  • Walz A, Seijin P, Slovin JP, Ludwig-Mueller J, Momonoki YS, Cohen JD (2002) A gene encoding a protein mod by the phytohormone indoleacetic acid. Proc Natl Acad Sci USA 99:1718–1723
  • Wolf B (1982) A comprehensive system of leaf analysis and its use for diagnosing crop nutrient status. Commun Soil Sci Plant Anal 13:1035–1059
  • Woodward AW, Bartel B (2005) Auxin: regulation, action and interaction. Ann Bot 95:707–735
rekord w opracowaniu
Typ dokumentu
Identyfikator YADDA
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.