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2011 | 14 | 3 |
Tytuł artykułu

A comparison of the effectiveness of the microscopic method and the multiplex PCR method in identifying and discriminating the species of Nosema spp. spores in worker bees (Apis mellifera)from winter hive debris

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Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
The objective of this study was to compare the effectiveness of the multiplex PCR method and traditional light microscopy in identifying and discriminating the species of Nosema spp. spores in worker bees from winter hive debris in the Province of Warmia and Mazury (NE Poland). A total of 1000 beesdead after from the bottom of the hive from bee colonies were analyzed. Spores were identified with the use of a light microscope (400-600x magnification). Spores were assigned to species by the multiplex PCR method. The microscopic evaluation revealed the presence of Nosema spp. spores in 803 samples (80.3%). Nosema ceranae spores were observed in 353 positive samples (43.96%), Nosema apis spores were found in 300 samples (37.35%), while 150 samples (19.67%) showed signs of a mixed infection. A multiplex PCR analysis revealed that 806 samples were infested with Nosema spp., of which 206 were affected only by Nosema ceranae, 600 showed signs of mixed invasion, while no samples were infected solely by Nosema apis parasites. In two cases, the presence of spores detected under a light microscope was not confirmed by the PCR analysis. The results of the study indicate that Nosema ceranae is the predominant parasitic species found in post-winter worker bees from the bottom of the hive in the region of Warmia and Mazury.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
14
Numer
3
Opis fizyczny
p.385-391,fig.,ref.
Twórcy
  • Department of Parasitologyand Invasiology, Faculty of Veterinary Medicine, University of Warmia nad Mazury in Olsztyn, Oczapowskiego 13, 10-718 Olsztyn, Poland
autor
Bibliografia
  • Chen Y, Evans JD, Smith IB, Pettis JS (2008) Nosema ceranae is a long-present and wide-spread microsporidian infection of the European honey bee (Apis mellifera) in the United States. J Invertebr Pathol 97: 186-188.
  • Chen YP, Evans JD, Murphy C, Gutell R, Zuker M, Gundensen-Rindal D, Pettis JS (2009a) Morphological, molecular, and phylogenetic characterization of Nosema ceranae, a microsporidian parasite isolated from the European honey bee, Apis mellifera. J Eukaryot Microbiol 56: 142-147.
  • Chen Y, Evans JD, Zhou L, Boncristiani H, Kimura K, Xiao T, Litkowski AM, Pettis JS (2009b) .Asymmetrical coexistence of Nosema ceranae and Nosema apis in honey bees. J Invertebr Pathol 101: 204-209.
  • Forsgren E, Fries I (2010) Comparative virulence of Nosema ceranae and Nosema apis in individual European honey bees. Vet Parasitol 170: 212-217.
  • Fries I (2010) Nosema ceranae in European honey bees (Apis mellifera). J Invertebr Pathol Suppl. 103: 573-79.
  • Gajda A (2010) Nosema ceranae w rodzinach pszczoły miodnej. Życie Weterynaryjne 85: 140-143.
  • Higes M, Martin-Hernández R, Meana A (2006). Nosema ceranae, a new microsporidian parasite in honeybees in Europe. J Invertebr Pathol 92: 93-95.
  • Higes M, Martin-Hernández R, Bottas C, Garrido- Bailón E, González-Porto V, Barrios L, del Nozal MJ, Bernal JL, Jiménez JJ, Garcia-Palencia P., Meana A. (2008) How natural infection by Nosema ceranae causes honeybee colony collapse. Environ Microbiol 10: 2659-2669.
  • Kasprzak S, Topolska G (2007) Nosema ceranae (Eukaryota: Fungi: Microsporea) – nowy pasożyt pszczoły miodnej Apis mellifera L. Wiad Parazyt 53: 281-284.
  • Martin-Hernández R, Meana A, Prieto L, Salvador A.M, Garrido-Bailón E, Higes M (2007) Outcome of colonization of Apis mellifera by Nosema ceranae. Appl Environ Microbiol 73: 6331-6338.
  • Office International des Epizooties (OIE) (2004) Manual of Standards for Diagnostic Test and Vaccines. [www document] URL http://www.oie.int./eng/normes/manual/A–00123.htm
  • Paxton R, Klee J, Korpela S, Fries I (2007) Nosema ceranae has infected Apis mellifera in Europe since at least 1998 and may be more virulent than Nosema apis. Apidologie 38: 558-565.
  • Tapaszti Z, Forgaach P, Kövágó C, Békési L, Bakonyi T, Rusvai M (2009) First detection and dominance of Nosema ceranae in Hungarian honeybee colonies. Acta Vet Hung 57: 383-388.
  • Topolska G, Hartwig A (2005) Diagnosis of Nosema apis infection by investigations of two kinds of samples: dead bees and live bees. J Apicult Sci 49: 75-79.
  • Topolska G, Kasprzak S (2007) Pierwsze przypadki zarażenia pszczół w Polsce przez Nosema ceranae. Med Weter (Suppl) 67: 1504-1506.
  • Webster TC, Pomper KW, Hunti G, Thacker EM, Jones SC (2004) Nosema apis infection in worker and queen Apis mellifera. Apidologie 35: 49-54.
Typ dokumentu
Bibliografia
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