PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2015 | 37 | 01 |

Tytuł artykułu

Genetic relationships and population structure of local olive tree accessions from Northeastern Spain revealed by SSR markers

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The olive (Olea europaea L. subsp. Europaea) is an old traditional crop which was domesticated from the wild at various locations around the Mediterranean basin, resulting in a vast number of accessions worldwide. This is the case of the northeast part of Spain (Aragon), where 163 genotypes were prospected and characterised. The majority of these genotypes (90 %) seem to be autochthonous accessions of this area and the rest are synonyms of accessions from other areas of Spain. In this study, 11 nuclear SSR markers were used to discriminate between the accessions and to understand the genetic relationship among them. All primers produced a successful amplification giving a total of 176 fragments in the genotypes studied, with an average of 16 alleles per SSR, ranging from 5 (DCA13) to 24 (DCA11). Allele size ranged from 120 bp at locus UDO99 to 284 bp at locus DCA15. The dendrogram generated using the variability observed classified most of the genotypes according to their geographical origin (Huesca, Teruel and Zaragoza provinces), confirming the particular evolution of different olive ecotypes. Structure software was used to investigate the genetic population structure among olive accessions, showing that the maximum rate of change in the log probability of the data occurred at K = 2. In addition, the SSR markers have consequently shown their usefulness for cultivar identification in olive, for establishing the genetic closeness among its accessions, and for establishing genealogical relationships.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

37

Numer

01

Opis fizyczny

Article: 1726 [12 p.], fig.,ref.

Twórcy

autor
  • Unidad de Hortofruticultura, Centro de Investigacion y Tecnología Agroalimentaria de Aragon (CITA), Av. Montanana 950, 50059 Saragossa, Spain
  • Unidad de Hortofruticultura, Centro de Investigacion y Tecnología Agroalimentaria de Aragon (CITA), Av. Montanana 950, 50059 Saragossa, Spain
  • Unidad de Hortofruticultura, Centro de Investigacion y Tecnología Agroalimentaria de Aragon (CITA), Av. Montanana 950, 50059 Saragossa, Spain
  • Unidad de Hortofruticultura, Centro de Investigacion y Tecnología Agroalimentaria de Aragon (CITA), Av. Montanana 950, 50059 Saragossa, Spain

Bibliografia

  • Albertini E, Torricelli R, Bitocchi E, Raggi L, Marconi G, Pollastri L, Di Minco G, Battistini A, Papa R, Veronesi F (2011) Structure of genetic diversity in Olea europaea L. accessions from central Italy. Mol Breed 27:533–547
  • Baldoni L, Tosti N, Ricciolini C, Belaj A, Arcioni S, Pannelli G, Germana MA, Mulas M, Porceddu A (2006) Genetic structure of wild and cultivated olives in the central Mediterranean basin. Ann Bot 98:935–942
  • Barranco D, Trujillo I, Rallo L (2005) Elaiografía Hispanica. In: Martín A, Tous J, Trujillo I, Rallo L, Caballero JM, del Río C (eds) Variedades de olivo en España. Junta de Andalucía- MAPA-Ed Mundi-Prensa, Madrid, pp 45–231
  • Belaj A, Trujillo I, de la Rosa R, Rallo L, Giménez MJ (2001) Polymorphism and discrimination capacity of randomly amplified polymorphic markers in olive germplasm bank. J Am Soc Hort Sci 126:64–71
  • Belaj A, Satovic Z, Cipriani G, Baldoni L, Testolin R, Rallo L, Trujillo I (2003) Comparative study of the discriminating capacity of RAPD AFLP and SSR markers and of their effectiveness in establishing genetic relationships in olive. Theor Appl Genet 107:736–744
  • Belaj A, Cipriani G, Testolin R, Rallo L, Trujillo I (2004) Characterization and identification of the main Spanish and Italian accessions by simple sequence repeat markers. Hort-Science 39:1557–1561
  • Belaj A, Muñoz-Díez C, Baldoni L, Porceddu A, Barranco D, Satovic Z (2007) Genetic diversity and population structure of wild olives from the north-western Mediterranean assessed by SSR markers. Ann Bot 100:449–458
  • Belaj A, Muñoz-Díez C, Luciana B, Zlatko S, Barranco D (2010) Genetic diversity and relationships of wild and cultivated olives at regional level in Spain. Sci Hort 124:323–330
  • Besnard G, Breton C, Baradat P, Khadari B, Berville A (2001) Cultivar identification in olive based on RAPD markers. J Am Soc Hort Sci 126:668–675
  • Breton C, Pinatel C, Médail F, Bonhomme F, Bervillé A (2008) Comparison between classical and Bayesian methods to investigate the history of olive cultivars using SSR-polymorphisms. Plant Sci 175:524–532
  • Brito G, Loureiro J, Lopes T, Rodriguez E, Santos C (2008) Genetic characterisation of olive trees from Madeira Archipelago using flow cytometry and microsatellite markers. Genet Resour Crop Evol 55:657–664
  • Brookfield JFY (1996) A simple new method for estimating null allele frequency from heterozygote deficiency. Mol Ecol 5:453–455
  • Carriero F, Fontanazza G, Cellini F, Giorio G (2002) Identification of simple sequence repeats (SSRs) in olive (Olea europaea L.). Theor Appl Genet 104:301–307
  • Cipriani G, Marrazzo MT, Marconi R, Cimato A, Testolin R (2002) Microsatellite markers isolated in olive are suitable for individual fingerprinting and reveal polymorphism within ancient cultivars (Olea europaea L). Theor Appl Genet 104:223–228
  • De la Rosa R, James C, Tobutt KR (2002) Isolation and characterization of polymorphic microsatellite in olive (Olea europaea L.) and their transferability to other genera in the Oleaceae. Mol Ecol Notes 2:265–267
  • Díez CM, Trujillo I, Barrio E, Belaj A, Barranco D, Rallo L (2011) Centennial olive trees as a reservoir of genetic diversity. Ann Bot 108:797–807 do Val ADB, Ferreira JL, Vieira Neto J, Pasqual M, de Oliveira AF, Borém A, Cançado GMA (2012) Genetic diversity of Brazilian and introduced olive germplasms based on microsatellite markers. Mol Res 11:556–571
  • Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11–15
  • Ercisli S, Ipek A, Barut E (2011) SSR marker-based DNA fingerprinting and cultivar identification of olives (Olea europaea L.). Biochem Genet 49:555–561
  • Ercisli S, Bencic D, Ipek A, Barut E, Liber Z (2012) Genetic relationships among olive (Olea europaea L.) cultivars native to Croatia and Turkey. J Appl Bot Food Qual 85:144–149
  • Erre P, Chessa I, Muñoz-Díez C, Belaj A, Rallo L, Trujillo I (2010) Genetic diversity and relationships between wild and cultivated olives (Olea europaea L.). Genet Resour Crop Evol 57:41–54
  • Espada JL, Gracia S (2009) Variedades de olivo cultivadas en el Somontano de Barbastro y características de sus aceites. Informaciones técnicas DGA 212
  • Evanno G, Regnaut S, Goudet J (2005) Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study. Mol Ecol 14:2611–2620
  • Falush D, Stephens M, Pritchard JK (2003) Inference of population structure using multilocus genotype data: linked loci and correlated allele frequencies. Genetics 164:1567–1587
  • FAOSTAT 2013. http://faostat.fao.org/
  • Fendri M, Ferreira E, Calado L, Abreu I, Rodríguez-García MI, Amorim MI, Alché JD (2014) Discrimination of Portuguese and Spanish olive cultivars using microsatellite markers. Acad J Agric Res 2:58–61
  • Ferreira dos Santos AR, Ramos-Cabrer AM, Díaz-Hernández MB, Pereira-Lorenzo S (2011) Genetic variability and diversification process in local pear cultivars from Northwestern Spain using microsatellites. Tree Genet Genomes 7:1041–1056
  • Flint-Garcia SA, Thornsberry JM, Buckler ES (2003) Structure of linkage disequilibrium in plants. Annu Rev Plant Biol 54:357–374
  • Font i Forcada C, Oraguzie N, Igartua E, Moreno MA, Gogorcena Y (2013) Population structure and marker-trait associations for pomological traits in peach and nectarine cultivars. Tree Genet Genomes 9:331–349
  • Harbi I, Al-Awadi SJ, Moner AIM (2012) Molecular characterization of olive cultivars in Iraq using SSR markers and compare with phenotypic characterization. J Life Sci 6:1343–1350
  • Horvath A, Balsemin E, Barbot JC, Christmann H, Manzano G, Reynet P, Laigret F, Mariette S (2011) Phenotypic variability and genetic structure in plum (Prunus domestica L.) cherry plum (P. cerasifera Ehrh.) and sloe (P. spinosa L.). Sci Hort 129:283–293
  • Kochmstedt AM, Aradhya MK, Soleri D, Smith JL, Polito VS (2012) Analysis of genetic diversity and structure of some historic olive (Olea europaea L.) germplasm in the United States using microsatellite markers. Acta Hort 948:159–168
  • Lavee S (1986) Olive. In: Monselise SP (ed) Handbook of fruit set and development. CRC Press, Boca Raton, pp 261–276
  • Lopes MS, Mendonca D, Sefc KM, Gil FS, Camara Machado A (2004) Genetic evidence of intra-cultivar variability within Iberian olive cultivars. HortScience 39:1562–1565
  • Mantel NA (1967) The detection of disease clustering and a generalized regression approach. Cancer Res 27:209–220
  • Mariette S, Tavaud M, Arunyawat U, Capdeville G, Millan M, Salin F (2010) Population structure and genetic bottleneck in sweet cherry estimated with SSRs and the gametophytic self-incompatibility locus. BMC Genet 11:77
  • Marra FP, Caruso T, Costa F, Di Vaio C, Mafrica R, Marchese A (2013) Genetic relationships structure and parentage simulation among the olive tree (Olea europaea L. subsp europaea) cultivated in Southern Italy revealed by SSR markers. Tree Genet Genomes 9:961–973
  • Muzzalupo I, Vendramin GG, Chiappetta A (2014) Genetic biodiversity of Italian olives (Olea europaea) germplasm analyzed by SSR markers. Sci World J. doi:10.1155/2014/296590
  • Nei M, Li WH (1979) Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc Natl Acad Sci USA 76:5269–5273
  • Newton AC, Allnutt AR, Gillies ACM, Lowe AJ, Ennos RA (1999) Molecular phylogeography intraspecific variation and the conservation of tree species. Trends Ecol Evol 14:140–145
  • Owen CA, Bita EC, Banilas G (2005) AFLP reveals structural details of genetic diversity within cultivated olive germplasm from the Eastern Mediterranean. Theor Appl Genet 110:1169–1176
  • Pereira-Lorenzo S, Costa RML, Ramos-Cabrer AM, Ribeiro CAM, da Silva MFS, Manzano G, Barreneche T (2010) Variation in grafted European chestnut and hybrids by microsatellites reveals two main origins in the Iberian Peninsula Tree Genet Genomes 6:701–715
  • Pritchard JK, Stephens M, Rosenberg NA, Donnelly P (2000) Association mapping in structured populations. Am J Hum Genet 67:170–181
  • Rallo P, Dorado G, Martin A (2000) Development of simple sequence repeats (SSRs) in olive tree (Olea europaea L). Theor Appl Genet 101:984–989
  • Rallo L, Barranco D, Caballero JM, Del Río C, Martín A, Tous J, Trujillo I (2005) Variedades de Olivo en España. Junta de Andalucía-Consejería de Agricultura y Pesca. Ministerio de Agricultura Pesca y Alimentación, Mundi Prensa, p 478
  • Roubos K, Moustakas M, Aravanopoulos FA (2010) Molecular identification of Greek olive (Olea europaea) cultivars based on microsatellite loci. Genet Mol Res 9:1865–1876
  • Sabino Gil F, Busconi MD, Camara Machado A, Fogher C (2006) Development and characterization of microsatellite loci from Olea europaea. Mol Ecol Notes 6:1275–1277
  • Sanz-Cortés F, Badenes ML, Paz S, Iñiguez A, Llácer G (2001) Molecular characterization of olive cultivars using RAPD markers. J Am Soc Hort Sci 126:7–12
  • Sarri V, Baldoni L, Porceddu A, Cultrera NGM, Contento A, Fedriani M, Belaj A, Trujillo I, Cionini PG (2006) Microsatellite markers are powerful tool for discriminating among olive and assigning them to geographically defined populations. Genome 49:1606–1615
  • Sefc K, Lopes MS, Mendonc¸a D, Rodrigues Dos Santos M, Da Câmara Laimer, Machado MD, Câmara Machado A (2000) Identification of microsatellite loci in olive (Olea europaea) and their characterization in Italian and Iberian olive trees. Mol Ecol 9:1171–1173
  • Shriner D, Vaughan LK, Padilla MA, Tiwari HK (2007) Problems with genome-wide association studies. Science 316:1840–1842
  • Sokal R, Rohlf FJ (1962) The comparison of dendrograms by objective methods. Taxon 11:33–40
  • Spennemann DHR, Allen LR (2000) Feral olives (Olea europaea) as future woody weeds in Australia: a review. Aust J Exp Agric 40:889–901
  • Tous J, Romero A (1993) Variedades del olivo. Fundación ‘La Caixa’, Barcelona Trujillo I, Rallo L, Arús P (1995) Identifying olive cultivars by isozyme analysis. J Am Soc Hort Sci 120:318–324
  • Trujillo I, Ojeda MA, Urdiroz NM, Potter D, Barranco D, Rallo L, Díez CM (2014) Identification of the worldwide olive germplasm bank of Córdoba (Spain) using SSR and morphological markers. Tree Genet Genomes 10:141–155
  • Viñuales J (2007) Variedades de olivo del Somontano. Premio Félix de Azara. Instituto de Estudios Altoaragoneses, Huesca Wright S (1951) The genetical structure of populations. Ann Eugenics 15:323–354
  • Yeh FC, Yang RC, Boyle T (1997) POPGENE the user-friendly shareware for population genetic analysis. Molecular Biology and Biotechnology Centre University of Alberta, Canada. http://www.ualberta.ca/~fyeh/
  • Zaher H, Boulouha B, Baaziz M, Sikaoui L, Gaboun F, Udupa SM (2011) Morphological and genetic diversity in olive (Olea europaea subsp europaea L.) clones and varieties. Plant Omics J 4:370–376

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-2796d1a8-33e7-408d-a60f-a55fc0780bf5
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.