Effect of selenium and vitamin E supplementation on semen quality in dogs with lowered fertility

• Autorzy: Domoslawska A. , Zdunczyk S. , Nizanski W. , Jurczak A. , Janowski T.
• Języki publikacji: EN

Abstrakty

EN

Thirty clinicalh healthy dogs with poor semen quality were used in the study. Fifteen dogs were supplemented daily with selenium (0.6 mg/kg organic selenium from yeast) and vitamin E (5 mg/kg) per os for 60 d. The control group ( 15 dogs) was not supplemented. Semen was collected from all dogs by manual manipulation on days 0, 30, 60, and 90. The sperm concentration and motility parameters were evaluated with a Hamilton Thome sperm analy ser, version IVOS 12.3. For the assessment of sperm morphology, Diff-Quik stain was used. The percentage of live and dead spermatozoa was estimated on dried smears stained with eosin-nigrosin. The concentration of spermatozoa, most motility parameters determined (PMOT, VSL, VCL, ALH, BCF, RAPID, MEDIUM, SLOW, and STATIC), and the percentage of spermatozoa morphologically normal and live increased significantly (P < 0.05) after 60 d of supplementation. In the control group, there were no changes in motility parameters while the concentration and total sperm count decreased over the duration of the study. In conclusion, supplementation with selenium and vitamin E for 60 d can improve the quality of semen in dogs with lowered fertility.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2015
• Tom: 59
• Numer: 1
• Opis fizyczny: p.85-90,ref.

Twórcy

autor

Domoslawska A.

• Department of Animal Reproduction with Clinic, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, 10-719 Olsztyn, Poland

autor

Zdunczyk S.

• Department of Animal Reproduction with Clinic, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, 10-719 Olsztyn, Poland

autor

Nizanski W.

• Department of Reproduction and Clinic for Farm Animals, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, 50-366 Wroclaw, Poland

autor

Jurczak A.

• Department of Animal Reproduction with Clinic, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, 10-719 Olsztyn, Poland

autor

Janowski T.

• Department of Animal Reproduction with Clinic, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, 10-719 Olsztyn, Poland

Bibliografia

• 1. Bayles B., Usatine R.: Evening primrose oil. Am Fam Physician 2009, 80, 1405-1408.
• 2. Boitani C., Puglisi R.: Selenium, a key element in spermatogenesis and male fertility. Adv Exp Med Biol 2008, 636, 65-73.
• 3. Burk R.F., Hill K.E.: Selenoprotein P-expression, functions, and roles in mammals. Biochim Biophys Acta 2009, 1790, 1441-1447.
• 4. Danikowski S., Sallmann H.P., Halle I., Flachowsky G.: Influence of high levels of vitamin E on semen parameters of cocks. J Anirn Physiol Anim Nutr 2002, 86, 376-382.
• 5. Da Rocha A.A., da Cunha I.C.N., Ederli B.B., Albernaz A.P., Quirino C.R.: Effect of daily food supplementation with essential fatty acids on canine semen quality. Reprod Dom Anim 2009, 44, Suppl. 22, 313-315.
• 6. Domoslawska A., Zdunczyk S., Nizanski W., Janowski T.: Assessment of semen quality in infertile dogs using computer assisted sperm analysis by the Hamilton-Thorne Semen Analyser. Bull Vet Inst Pulawy 2013, 57, 429-432.
• 7. Donnely E.D., Lewis S.E.M., McNally J.A., Thompson W.: In vivo fertilization and pregnancy rates: the influence of sperm motility and morphology on IVF outcome. Fert Steril 1998, 70, 304-314.
• 8. Feldmann J., Nelson R.W.: Canine and feline endocrinology and reproduction. W.B. Saunders, Philadelphia, 1996, pp. 718-733.
• 9. Fetterlof P.M., Rogers B.J.: Prediction of human sperm penetrating ability using computerized motion parameters. Mol Reprod Dev 1990, 27, 326-331.
• 10. Fontbonne A.: Infertility in male dogs: recent advances. Rev Bras Reprod Anim 2011, 35, 266-273.
• 11. Hatamoto L.K., Sobrinho B.C.A., Nichi N., Barnabe V.H., Barnabe R.C., Cortada C.N.M.: Effect of dexamethasone treatment (to mimic stress) and vitamin E oral supplementation on the spermogram and on seminal plasma spontaneous lipid peroxidation and antioxidant enzyme activities in dogs. Theriogenology 2006, 66, 1610-1644.
• 12. Hawkes W.C., Turek P.: Effects of dietary selenium on sperm motility in healthy men. J Androl 2001, Vol 22 (5),764-772.
• 13. Henson M.C., Kattesh H.G., Hitchcock J.P., Kincaid S.A.: The effect of dietary selenium on growth and selected reproductive parameters in y oung boars. Anim Prod 1983, 37, 401-407.
• 14. Iguer-Ouada M., Verstegen J.: Evaluation of the Hamilton Thorn computer-based automated system for dog semen analysis. Theriogenology 2001, 55, 733-749.
• 15. Iwanier K., Zachara B.A.: Selenium supplementation enhances the element concentration in blood and seminal fluid but does not change the spermatozoal quality characteristics in subfertile men. J Androl 1995, 16,441-447.
• 16. Keskes-Anmar L., Feki-Chakroun N., Rebai T., Sahnoun Z., Ghozzi H., Hammami S., Zghal K., Feki H., Damak J., Bahloul A.: Sperm oxidative stress and the effect of an oral vitamin E and selenium supplement on semen quality in infertile men. Arch Androl 2002, 49, 83-94.
• 17. Klimowicz M., Nizanski W., Savic M., Zbyryt I., Dubiel A.: Evaluation of dog semen quality using a conventional microscopic method, flow cytometry and HTM IVOS computer sperm quality analyzer. Med Weter 2005, 61, 1250-1255.
• 18. Kołodziej A., Jacyno E.: Effect of selenium and vitamin E supplementation on reproductive performance of young boars. Arch Tierz 2005, 48, 68-75.
• 19. Koziorowska-Gilun M., Strzeżek R.: Molecular forms of selected antioxidant enzy mes in dog semen - electrophoretical identification. Pol J Vet Sci 2011, 14, 29-33.
• 20. Krol M.B., Gromadzinska J., Wasowicz W.: SeP, ApoER2 and megalin as necessary factors to maintain Se homeostasis in mammals. J Trace Elem Med Biol 2012, 26, 161-266.
• 21. l.inde-Forsberg C.: Achieving canine pregnancy using frozen and chilled extended semen. Vet Clin North Am Small Anim Pract 2001, 21, 467-485.
• 22. Luberda-Bieńkowska Z., Majewska A.: Wpływ selenu i niskocząsteczkowych antyoksydantów na jakość nasienia zwierząt gospodarskich. Post Nauk Rol 2010, 4, 81-90.
• 23. Marin-Guzman, J., Mahan D.C., Chung Y.K., Pate J.L., Pope W.F.: Effects of dietary selenium and vitamin E on boar performance and tissue responses, semen quality, and subsequent fertilization rates in mature gilts. J Anim Sci 1997, 75, 2994-3003.
• 24. Marin-Guzman J., Mahan D.C., Whitmoyer R.: Effect of dietary selenium and vitamin E on the ultrastructure and ATP concentration of boar spermatozoa, and the efficacy of added sodium selenite in extended semen on sperm motility. J Anim Sci 2012, 78, 1544-1550.
• 25. Mickelsen W.D., Memon M.A., Anderson P.B., Freeman D.A.: The relationship of semen quality to pregnancy rate and litter size following artificial insemination in the bitch. Theriogenology 1993, 39, 553-560.
• 26. Moslemi M.K., Tavanbakhsh S.: Selenium-vitamin E supplementation in infertile men: effect on semen parameters and pregnancy rate. Int J Gen Med 2011, 4, 99-104.
• 27. Nielsen F., Mikkelsen B.B., Nielsen J.B., Andersen H.R., Grandjean P.: Plasma malondialdehyde as biomarker for oxidative stress: reference interval and effects of life-style factors. Clin Chem 1997, 43, 1209-1214.
• 28. Niżański W., Klimowicz M., Partka A., Savic M., Dubiel A.: Effects of inclusion of Equex STM into tris-based extender on the motility of dog spermatozoa incubated at 5 degrees C. Reprod Dom Anim 2009, 44, 363-365.
• 29. Niżański W., Partyka A., Ochota M., Antończyk A., Mikołajewska N., Błasiak K., Mila H., Stańczyk E.: Flow cytometric, computer assisted and traditional sperm analysis in fertile and subfertile dogs. Proc. 14th EVSSAR Congress, Milano 2011, p. 52.
• 30. Rijselaere T., Maes D., Hoflack G., de Kruif A., Van Soom A.: Effect of body weight, age and breeding history on canine sperm quality parameters measured by the Hamilton-Thorne analyser. Reprod Dom Anim 2007, 42, 143-148.
• 31. Sallam H.N., Ezzeldin F., Sallam A., Agameya A.F., Farrag A.: Sperm velocity and morphology, female characteristics, and the hy po-osmotic swelling test as predictors of fertilization potential: experience from the IVF model. Int J Fertil Womens Med 2003, 48, 88-95.
• 32. Scott R., MacPherson A., Yates R.W., Hussain B., Dixon J.: The effect of oral selenium supplementation on human sperm motility. Br J Urol 1998, 82, 76-80.
• 33. Słowińska M., Jankowski J., Dietrich G.J., Karol H., Liszewska E., Kozłowski K., Saratowska K., Ciereszko A.: Effect of organic and inorganic forms of selenium in diets on Turkey semen quality. Poultry Sci 2011, 90, 181-190.
• 34. Speight S.M., Estienne M.J., Harper A.F., Crawford R.J., Knight J.W., Whitaker B.D.: Effects of dietary supplementation with an organic source of selenium on characteristics of semen quality and in vitro fertility in boars. J Anim Sci. 2012, 90, 761-70.
• 35. Strzeżek R., Koziorowska-Gilun M., Kowalówka M., Strzeżek J.: Characteristics of antioxidant system in dog semen. Pol J Vet Sci 2009, 12, 55-60.
• 36. Suleiman S.A., Ali M.E., Zaiu Z.M.S., El-Malik E.M.A., Nasr M.A.: Lipid peroxidation and human sperm motility: protective role of vitamin E. J Androl 1996, 17, 530-537.
• 37. Udała J., Ramisz A., Drewnowski W., Lasota B., Radoch W.: The semen quality of bulls after application of selenium and vitamin E.Zesz Nauk AR Szczecin 1995, 168, 57-63.
• 38. Verstegen J., Iguer-Ouada M., Onclin M.: Computer assisted semen analyzers in andrology research and veterinary practice. Theriogenology 2002, 57, 149-179.
• 39. Watanabe T., Endo A.: Effect of selenium deficiency on sperm morphology and spermatocyte chromosomes in mice. Mutat Res 1991, 262, 93-99.

Identyfikatory

DOI

10.1515/bvip-2015-0013