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2012 | 19 | 1 |
Tytuł artykułu

Antibiotic resistance of Escherichia coli isolated from a poultry slaughterhouse

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
The aim of the study was to investigate the antibiotic resistant E. coli strains isolated from bioaerosols and surface swabs in a slaughterhouse as a possible source of poultry meat contamination. The highest air coliforms contamination was during shackling, killing and evisceration of poultry. The strains showed resistance to ampicillin (89%), ceftiofur (62%) and cefquinome (22%), while resistance to ampicillin with sulbactam was only 6%. Resistance to streptomycin and gentamicin was detected in 43% vs. 14% isolates; to tetracycline 33%; to chloramphenicol and florfenicol in 10% vs. 18% isolates; to cotrimoxazol in 35% isolates; to enrofloxacin in 43 % isolates. The higher MIC of ceftazidime (3.6 mg.l-1) and ceftriaxon (5.2 mg.l-1) revealed the presence of ESBLs in 43% of isolates. From 19 selected phenotypically ESBL positive strains, 16 consisted of CMY-2 genes, while CTX-M genes were not detected by PCR. Maldi tof analysis of selected E. coli showed a clear clonal relatedness of environmental strains from various withdrawals.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
19
Numer
1
Opis fizyczny
p.75-77,fig.,ref.
Twórcy
autor
  • University of Veterinary Medicine and Pharmacy, Kosice, Slovakia
autor
  • Faculty of Medicine, Department of Medical and Clinical Microbiology, P. J. Safarik University, Kosice, Slovakia
autor
  • Institute of Animal Physiology, Slovak Academy of Sciences, Kosice, Slovakia
  • University of Veterinary Medicine and Pharmacy, Kosice, Slovakia
autor
  • Slovak University of Agriculture, Nitra, Slovakia
Bibliografia
  • 1. Tsola E, Drosinos EH, Zoiopoulos P. Impact of poultry slaughter house modernisation and updating of food safety management systems on the microbiological quality and safety of products. Food Control. 2008; 19: 423-431.
  • 2. Dieckmann R, Malorny B. Rapid screening of epidemiologically important Salmonella enterica subsp. enterica serovars by wholecell matrix-assisted laser desorption ionization–time of flight mass spectrometry. Appl Environ Microbiol. 2011; 77: 4136-4146.
  • 3. Sauer S, Freiwald A, Maier T, Kube M, Reinhardt R, Kostrzewa M, Geider K. Classification and identification of bacteria by mass spectrometry and computational analysis. PLoS One. 2008; 3(e2843): 1-10.
  • 4. Clinical Laboratory Standards Institute. Performance Standards for Antimicrobial Disk and Dilution. Susceptibility Tests for BacteriaIsolated from Animals (3rd edn.). CLSI Document M31-A3 28, 2008: 1-99.
  • 5. Gattringer R, Niks M, Ostertag R, Schwarz K, Medvedovic H, Graninger W, Georgopoulos A. Evaluation of Miditech automated colorimetric MIC reading for antimicrobial susceptibility testing. J Antimicrob Chemother. 2002; 49: 651-659.
  • 6. Livermore DM, Winstanley TG, Shannon KP. Intepretative reading: recognising unusual and interferring resistance mechanisms from resistance phenotypes. J Antimicrob Chemother. 2001; 48(Suppl. S1): 87-102.
  • 7. Carattoli A, Garcia-Fernandez A, Varesi P, Fortini D, Gerardi S, Penni A, Mancini C, Giordano A. Molecular epidemiology of Escherichia coli producing extended-spectrum ß-lactamases isolated in Rome, Italy. J Clin Microbiol. 2008; 46: 103-108.
  • 8. Pérez-Pérez PJ, Hanson ND. Detection of plasmid-mediated AmpC beta-lactamase genes in clinical isolates by using multiplex PCR. J ClinMicrobiol. 2002; 40: 2153-2162.
  • 9. Bolder MN. The microbiology of the slaughter and processing of poultry. In: Davies A, Board R (Ed): The Microbiology of Meat and Poultry, 158-173, Springer – Verlag, Heidelberg, 1998.
  • 10. Fernandez-Lopez J, Sendra-Nadal E, Sayas-Barbera E. Slaughtering equipment and operations. In: Guerrero-Legarreta I (Ed.): Handbookof Poultry Science and Technology, Primary Processing. John Wiley &Sons, Inc., New Jersey 2009: 79-100.
  • 11. Marriott NG. Essentials of Food Sanitation, Sources of Food Contamination. Chapman and Hall, New York 1997.
  • 12. Brtkova A, Bujdakova H. Antibiotic resistance in Enterococcus isolates from poultry swabs in Slovakia. J Food Nutr Res. 2009; 48: 121-128.
  • 13. Drugdova Z, Kmet V, Bujnakova D. Virulence factors in Escherichia coli isolated from chicken meat in Slovakia. J Food Nutr Res. 2010; 49: 10-13.
  • 14. Kmet V, Bujnakova D, Drugdova Z, Kmetová M. Betalactam resistance in food Escherichia coli isolated from broilers. In: Mendez-Vilas A (Ed.): Microorganisms in Industry and Environment. From Scientific and Industrial Research to Consumer Products, World Scientific Pub Co. Pte Ltd, Singapore 2011: 363-365.
  • 15. Warren RE, Ensor VM, O’Neill P, Butler V, Taylor J, Nye K, Harvey M, Livermore DM, Woodford N, Hawkey PM. Imported chicken meat as a potential source of quinolone-resistant Escherichia coli producing extended-spectrum ß-lactamases in the UK. J Antimicrob Chemother. 2008; 61: 504-508.
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rekord w opracowaniu
Typ dokumentu
Bibliografia
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Identyfikator YADDA
bwmeta1.element.agro-27574cef-ff44-4435-9b4f-1f03bd7cac3d
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