Reactive oxygen species production and antioxidative defense system in pea root tissues treated with lead ions: the whole roots level

• Autorzy: Malecka A. , Piechalak A. , Tomaszewska B.
• Języki publikacji: EN

Abstrakty

EN

The lead absorbed by the roots induce oxidative stress conditions through the Reactive oxygen species (ROS) production for the pea plants cultivated hydroponically for 96 h on a Hoagland medium with the addition of 0.1 and 0.5 mM of Pb(NO₃)₂. The alterations in O₂⁻˙ and H₂O₂ concentrations were monitored spectrophotometrically which show a rapid increase in O₂⁻˙ production during the initial 2 h, and in case of H₂O₂, during the eighth hour of cultivation. The level of ROS remained higher at all the time points for the roots of the plants cultivated with Pb²⁺ and it was proportional to metal concentration. The production of O₂⁻˙ and H₂O₂ was visualized by means of fluorescence microscope technique. They are produced in nonenzymatic membrane lipid peroxidation and its final product is Malondialdehyde, the level of which increased together with the level of H₂O₂. As stress intensity raised (duration of treatment and Pb²⁺ concentration), so did the activities of superoxide dismutases, catalase and ascorbate peroxidase antioxidative enzymes and of low-molecular antioxidants, particularly glutathione (GSH), homoglutathione (h-GSH) and cysteine substrate toward their synthesis. The root cells redox state (GSH/GSSG) dropped proportionally to lead stress intensity.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2009
• Tom: 31
• Numer: 5
• Opis fizyczny: p.1053-1063,fig.,ref.

Twórcy

autor

Malecka A.

• Department of Biochemistry, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University, Collegium Bilogicum, Umultowska 89, 61-614 Poznan, Poland

autor

Piechalak A.

• Department of Biochemistry, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University, Collegium Bilogicum, Umultowska 89, 61-614 Poznan, Poland

autor

Tomaszewska B.

• Department of Biochemistry, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University, Collegium Bilogicum, Umultowska 89, 61-614 Poznan, Poland

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