Assessment of lipid and protein peroxidation markers in non-pregnant and pregnant female dogs

• Autorzy: Szczubial M. , Kankofer M. , Dabrowski R. , Bochniarz M. , Urban-Chmiel R.
• Języki publikacji: EN

Abstrakty

EN

The aim of the study was to investigate oxidative stress during normal pregnancy in female dogs based on an evaluation of plasma markers for lipid and protein peroxidation. Twenty clinically healthy female dogs (10 non-pregnant and 10 pregnant) were used in the study. Blood samples from the pregnant animals were collected at 19-21, 38-40, and 56-58 days of pregnancy. Blood samples from non-pregnant female dogs were obtained between 20 and 35 days after ineffective breeding. As indicators of oxidative stress, we measured the following using spectrophotometric and spectrof-luorimetric methods: thiobarbituric acid reactive substances (TBARS), radical cations of N,N, die-thylpara-phenylene diamine (RC-DEPPD), sulfhydryl groups (SH groups), bityrosine and formyl-kynurenine. The mean plasma TBARS concentration in the pregnant dogs (0.486 ± 0.071 - 0.581 ± 0.191 |imol/g protein) was significantly higher (p<0.05) than that found in the non-pregnant animals (0.274 ± 0.111 |imol/g protein). A marked, although not significant, decrease in SH group content, as well as an increase in bityrosine and formylkynurenine concentration were concurrently observed in the pregnant dogs. No significant differences were found in terms of the studied markers in the pregnant animals when comparing the values obtained during the investigated periods of pregnancy, although there was a progressive decrease in TBARS concentration and a progressive increase in RC-DEPPD, bityrosine and formylkynurenine contents. Our findings suggest that normal pregnancy in female dogs is associated with oxidative stress. Further studies are necessary to establish the physiological ranges of antioxidative/oxidative profiles in pregnant dogs and to explain if and how the intensity of oxidative stress might contribute to the risk of the complications of pregnancy.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2015
• Tom: 18
• Numer: 1
• Opis fizyczny: p.171-179,ref.

Twórcy

autor

Szczubial M.

• Department and Clinic of Animal Reproduction, Faculty of Veterinary Medicine, University of Life Sciences, Gleboka 30, 20-612 Lublin, Poland

autor

Kankofer M.

• Department of Animal Biochemistry, Faculty of Veterinary Medicine, University of Life Sciences, Akademicka 12, 20-033 Lublin, Poland

autor

Dabrowski R.

• Department and Clinic of Animal Reproduction, Faculty of Veterinary Medicine, University of Life Sciences, Gleboka 30, 20-612 Lublin, Poland

autor

Bochniarz M.

• Department and Clinic of Animal Reproduction, Faculty of Veterinary Medicine, University of Life Sciences, Gleboka 30, 20-612 Lublin, Poland

autor

Urban-Chmiel R.

• Sub-department of Veterinary Prevention and Avian Diseases, Institute of the Biological Bases of Animal Diseases, Faculty of Veterinary Medicine, University of Life Sciences, Akademicka 12, 20-033 Lublin, Poland

Bibliografia

• Agarwal A, Gupta S, Sharma RK (2005) Role of oxidative stress in female reproduction. Reprod Biol Endocrinol 3: 28-48.
• Alberti A, Bolognini L, Macciantelli D, Caratelli M (2000) The radical cation of N,N-diethyl-para-phenylendiamine: a possible indicator of oxidative stress in biological samples. Res Chem Intermed 26: 253-267.
• Al-Gubory KH, Fowler PA, Garrel C (2010) The roles of cellular reactive oxygen species, oxidative stress and anti-oxidants pregnancy outcomes. Int J Biochem Cell Biol 42: 1634-1650.
• Al-Naemi RS, Abdullah QH, Ibrahim SA (2012) Impact of oxidative stress on pregnancy outcome in albino rats. Iraqi J Vet Sci 26: 93-99.
• Al-Qudah KM (2011) Oxidant and antioxidant profile of hyperketonemic ewes affected by pregnancy toxemia. Vet Clin Pathol 40: 60-65.
• Aw TY (1998) Determinants of intestinal detoxication of lipid hydroperoxides. Free Radic Res 28: 637-646.
• Beal MF (2002) Oxidatively modified proteins in aging and disease. Free Radic Biol Med 32: 797-803.
• Bedaiwy MA, Falcone T, Mohamed MS, Aleem AA, Sharma RK, Worley SE, Thornton J, Agarwal A (2004) Differential growth of human embryos in vitro: role of reactive oxygen species. Fertil Steril 82: 593-600.
• Benzie IF (1996) Lipid peroxidation: a review of causes, consequences, measurement and dietary influences. Int J Food Sci Nutr 47: 233-261.
• Berchieri-Ronchi CB, Kim SW, Zhao Y, Correa CR, Yeum KJ, Ferreira AL (2011) Oxidative stress status of highly prolific sows during gestation and lactation. Animal 5: 1774-1779.
• Cadenas S, Aragones J, Landazuri MO (2010) Mitochondrial reprogramming through cardiac oxygen sensors in ischaemic heart disease. Cardiovasc Res 88: 219-228.
• Carine D, Loverco G, Greko P, Capuno F, Selvaggi L (1993) Lipid peroxidation products and antioxidant enzymes in red blood cells during normal and diabetic pregnancy. Eur J Obstet Gynecol Reprod Biol 51: 103-109.
• Concannon PW, Castracane VD, Temple M, Montanez A (2009) Endocrine control of ovarian function in dogs and other carnivores. Anim Reprod 6: 172-193.
• Djordjevic A, Spasic S, Jovanovic-Galovic A, Djordjevic R, Grubor-Lajsic G (2004) Oxidative stress in diabetic pregnancy: SOD, CAT, and GSH-Px activity and lipid peroxi-dation products. J Matern Fetal Neonatal Med 16: 367-372.
• Erisir M, Benzer F, Kandemir FM (2009) Changes in the rate of lipid peroxidation in plasma and selected blood antioxidants before and during pregnancy in ewes. Acta Vet Brno 78: 237-242.
• Garrel C, Fowler PA, Al-Gubory KH (2010) Developmental changes in antioxidant enzymatic defences against oxidative stress in sheep placentomes. J Endocrinol 205: 107-116.
• Gitto E, Reiter RJ, Karbownik M, Tan DX, Gitto P, Barberi S, Barberi I (2002) Causes of oxidative stress in the pre-and perinatal period. Biol Neonate 81: 146-157.
• Goldstein S, Czapski G, Cohen H, Meyerstein D (1994) Free radicals induced peptide damage in the presence of transition metal ions: a plausible pathway for biological deleterious processes. Free Radic Biol Med 17: 11-18.
• Gutteridge JM (1995) Lipid peroxidation and antioxidants as biomarkers of tissue damage. Clin Chem 41: 1819-1828.
• Halliwell B (2006) Reactive species and antioxidants. Redox biology is a fundamental theme of aerobic life. Plant Physiol 141: 312-322.
• Halliwell B, Whiteman M (2004) Measuring reactive species and oxidative damage in vivo and in cell culture: how should you do it and what do the results mean? Br J Pharmacol 142: 231-255.
• Hung TH, Lo LM, Chiu TH, Li MJ, Yeh YL, Chen SF, Hsieh TT (2010) A longitudinal study of oxidative stress and antioxidant status in woman with uncomplicated pregnancies throughout gestation. Reprod Sci 17: 401-409.
• Idonije OB, Festus O, Okhiai O, Akpamu U (2011) A comparative study of the status of oxidative stress in pregnant Nigerian women. Res J Obstet Gynecol 4: 28-36.
• Iioka H (1994) Changes in blood level of lipid peroxide and vitamin E during pregnancy: clinical significance and relation to the pathogenesis of EPH gestosis. Gynecol Obstet Invest 38: 173-176.
• Jauniaux E, Burton GJ (2005) Pathophysiology of histological changes in early pregnancy loss. Placenta 26: 114-123.
• Joshi A, Kataria N, Sharma A, Arora S, Abhimanu, Maan R, Asopa S, Arya N, Khan S, Kataria AK (2013) Detection of oxidative stress in pregnant and non-pregnant Murrah Buffaloes during extreme ambiences. Vet Res 6: 27-31.
• Kim A, Murphy MP, Oberley TD (2005) Mitochondrial redox state regulates transcription of the nuclear-encoded mitochondrial protein manganese superoxide dismutase: a proposed adaptative response to mitochondrial redox imbalance. Free Radic Biol Med 38: 644-654.
• Kruidenier L, Verspaget HW (2002) Review article: oxidative stress as a pathogenic factor in inflammatory bowel disease - radicals or ridiculous? Aliment Pharmacol Ther 16: 1997-2015.
• Kulcsar M, Danko G, Delavaud C, Mircu C, Nikolic AJ, Gaspardy A, Cernescu H, Chilliard Y, Cseh S, Rudas P, Huszenicza G (2006) Endocrine characteristics of late pregnant hyperketonaemic ewes and their reproductive performance following the induction of ovarian cyclicity out of the breeding season. Acta Vet Hung 54: 235-249.
• Ledwozyw A, Michalak J, Stepien A, Kadziolka A (1986) The relationship between plasma triglycerides, cholesterol, total lipids and lipid peroxidation products during human artheriosclerosis. Clin Chim Acta 155: 275-283.
• Lykkesfeldt J, Svendsen O (2007) Oxidants and antioxidants in disease: oxidative stress in farm animals. Vet Journal 173: 502-511.
• Marnett LJ, Riggins JN, West JD (2003) Endogenous generation of reactive oxidants and electrophiles and their reactions with DNA and protein. J Clin Invest 111: 583-593.
• Min J, Park B, KimYJ, Lee H, Ha E, Park H (2009) Effect of oxidative stress on birth sizes: consideration of window from mid pregnancy to delivery. Placenta 30: 418-423.
• Mohebbi-Fani M, Mirzaei A, Nazifi S, Shabbooie Z (2012) Changes of vitamins A, E, and C and lipid peroxidation status of breeding and pregnant sheep during dry seasons on medium-to-low quality forages. Trop Anim Health Prod 44: 259-265.
• Mutinati M, Piccinno M, Roncetti M, Campanile D, Rizzo A, Sciorsci R (2013) Oxidative stress during pregnancy in the sheep. Reprod Domest Anim 48: 353-357.
• Myatt L, Cui X (2004) Oxidative stress in the placenta. His-tochem Cell Biol 122: 369-382.
• Oztabak K, Civelek S, Ozpinar A, Burcak G, Esen F (2005) The effects of energy restricted diet on the activities of plasma Cu-Zn SOD, GSH-Px, CAT and TBARS concentrations in late pregnant ewes. Turk J Vet Anim Sci 29: 1067-1071.
• Pajovič SB, Saicić ZS, Spasić MB, Petrovič VM (2003) The effect of ovarian hormones on antioxidant enzyme activities in the brain of male rats. Physiol Res 52: 189-194.
• Qanungo S, Mukherjea M (2000) Ontogenic profile of some antioxidants and lipid peroxidation in human placental and fetal tissues. Mol Cell Biochem 215: 11-19.
• Rice-Evans CA, Diplock AT, Symons MC (1991) Techniques in free radical research. In: Burdon RH, van Knip-penberg PH (eds) Laboratory techniques in biochemistry and molecular biology. Elsevier Science Publishers BV, Amsterdam.
• Roof RL, Hall ED (2000) Gender differences in acute CNS trauma and stroke: neuroprotective effects of estrogen and progesterone. J Neurotrauma 17: 367-388.
• Simcock SE, Rutherfurd SM, Wester TJ, Hendriks WH (2005) Total selenium concentrations in canine and feline foods commercially available in New Zealand. New Zeal Vet J 53: 1-5.
• Staicu ML, Muresan A, Tache S, Moldovan R (2011) Effects of exogenous antioxidants on oxidative stress in pregnancy. J Med Life 4: 163-167.
• Suhail M, Patil S, Khan S, Siddiqui S (2011) Antioxidant vitamins and lipoperoxidation in non-pregnant,pregnant, and gestational diabetic women: erythrocytes osmotic fragility profiles. J Clin Med Res 2: 266-273.
• Todd SE, Thomas DG, Bosh G, Hendriks WH (2012) Selenium status in adult cats and dogs fed high levels of dietary inorganic and organic selenium. J Anim Sci 90: 2549-2555.
• Toescu V, Nuttall SL, Martin U, Kendall MJ, Dunne F (2002) Oxidative stress and normal pregnancy. Clin Endocrinol (Oxf) 57: 609-613.
• Toy H, Camuzcuoglu H, Celik H, Erel O, Aksoy N (2009) Assesment of serum paraoxonase and arylesterase activities in early pregnancy failure. Swiss Med Wkly 139: 76-81.
• Uotila J, Tuimala R, Aarnio T, Pyykko K, Ahotupa M (1991) Lipid peroxidation products, selenium-dependent glutathione peroxidase and vitamin E in normal pregnancy. Eur J Obstet Gynecol Reprod Biol 42: 95-100.
• Vannucchi CI, Jordao AA, Vannucchi H (2007) Antioxidant compounds and oxidative stress in female dogs during pregnancy. Res Vet Sci 83: 188-193.
• Wang Y, Walsh SW (1998) Placental mitochondria as a source of oxidative stress in pre-eclampsia. Placenta 19: 581-586.
• Wassmann K, Wassmann S, Nickenig G (2005) Progesterone antagonizes the vasoprotective effect of estrogen on antioxidant enzyme expression and function. Circ Res 97: 1046-1054.
• Watson AL, Palmer ME, Jauniaux E, Burton GJ (1997) Variations in expression of copper/zinc superoxide dismutase in villous trophoblast of the human placenta with gestational age. Placenta 18: 295-299.
• Wender-Ozegowska E, Kozlik J, Biczysko R, Ozegowski S (2004) Changes of oxidative stress parameters in diabetic pregnancy. Free Radic Res 38: 795-803.
• Wickens D, Wilkins MH, Lunec J, Ball G, Dormandy TL (1981) Free radical oxidation (peroxidation) products in plasma in normal and abnormal pregnancy. Ann Clin Biochem 18: 158-162.
• Wisdom SJ, Wilson R, McKillop JH, Walker JJ (1991) Antioxidant systems in normal pregnancy and in pregnancy-induced hypertension. Am J Obstet Gynecol 165: 1701-1704.

Identyfikatory

DOI

10.1515/pjvs-2015-002