Developmental competence of porcine chimeric embryos produced by aggregation

• Autorzy: Li J. , Jakobsen J.E. , Xiong Q. , Fu H. , Callesen H.
• Języki publikacji: EN

Abstrakty

EN

The purpose of our study was to compare the developmental competence and blastomere allocation of porcine chimeric embryos formed by micro-well aggregation. Chimeras were created by aggregating either two blastomeres originating from 2-cell embryos or two whole embryos, where embryos were produced either by parthenogenetic activation (PA) or handmade cloning (HMC).Results showed that the developmental competence of chimeric embryos, evaluated based on their blastocyst rate and total cell number per blastocyst, was increased when two whole 2-cell stage embryos (PA or HMC) were aggregated. In comparison, when two blastomeres were aggregated,the developmental competence of the chimeric embryos decreased if the blastomeres were either from PA or from HMC embryos, but not if they were from different sources, i.e. one PA and one HMC blastomere. To evaluate the cell contribution in embryo formation, aggregation was made with HMC embryos cloned using EGFP transgenic cells; the cell contribution in the formation of the inner cell mass or trophectoderm was random in chimeric blastocysts. Finally, two blastomeres from 2-cell stage embryos were fused to construct tetraploid embryos, and when diploid and tetraploid embryos were used for aggregation, the cells of the ICM and its close vicinity were mostly formed by blastomeres from the diploid embryo.

• Wydawca: -
• Czasopismo: Animal Science Papers and Reports
• Rocznik: 2015
• Tom: 33
• Numer: 3
• Opis fizyczny: p.213-222,fig.,ref.

Twórcy

autor

Li J.

• College of Animal Science and Technology, Nanjing Agricultural University, Nanjing Weigang 1, Jiangsu Province, China

autor

Jakobsen J.E.

• Department of Biomedicine, Aarhus University, DK-8000 Aarhus C, Denmark

autor

Xiong Q.

• College of Animal Science and Technology, Nanjing Agricultural University, Nanjing Weigang 1, Jiangsu Province, China

autor

Fu H.

• College of Animal Science and Technology, Nanjing Agricultural University, Nanjing Weigang 1, Jiangsu Province, China

autor

Callesen H.

• Department of Animal Science, Aarhus University, DK-8830 Tjele, Denmark

Bibliografia

• Boediono A., Suzuki T., Li L.Y., Godke RA., 1999 – Offspring born from chimeras reconstructed from parthenogenetic and in vitro fertilized bovine embryos. Molecular Reproduction and Development 53(2), 159-170.
• Boiani M., Eckardt S., Leu N.A., Schöler H.R., McLaughlin K.J., 2003 –Pluripotency deficit in clones overcome by clone-clone aggregation: epigenetic complementation? The EMBO Journal 22(19), 5304-5312.
• Chrenek P., Makarevich A.V., Production of rabbit chimeric embryos by aggregation of zona-free nuclear transfer blastomeres. Zygote 13(1): 39-44.
• Du Y., Li J., Kragh P.M., Zhang Y., Schmidt M., Břgh I.B., Zhang X., Purup S.,Kuwayama M., Jřrgensen A.L., Pedersen A.M., Villemoes K., Yang H., Bolund L., Vajta G., 2007 – Piglets born from vitrified cloned blastocysts produced with a simplified method of delipation and nuclear transfer. Cloning and Stem Cells 9(4), 469-476.
• Everett C.A., West J.D., 1996 – The influence of ploidy on the distribution of cells in chimaeric mouse blastocysts. Zygote 4(1), 59-66.
• Fehilly C.B., Willadsen S.M., Tucker E.M., 1984 – Interspecific chimaerism between sheep and goat. Nature 307(5952), 634-6.
• Giraldo A.M., Hylan D.A., Ballard C.B., Purpera M.N., Vaught T.D., Lynn J.W.,Godke R.A., Bondioli K.R., 2008 – Effect of epigenetic modifications of donor somatic cells on the subsequent chromatin remodeling of cloned bovine embryos. Biology of Reproduction 78(5),832-40.
• He W., Kong Q., Shi Y., Xie B., Jiao M., Huang T., Guo S., Hu K., Liu Z., 2013 – Generation and developmental characteristics of porcine tetraploid embryos and tetraploid chimeric embryos.Genomics Proteomics & Bioinformatics 11(5), 327-33.
• Hossain M.M., Tesfaye D., Salilew -Wondim D., Held E., Pröll M.J., Rings F., Kirfel G., Looft C., Tholen E., Uddin J., Schellander K., Hoelker M.,2013 – Massive deregulation of miRNAs from nuclear reprogramming errors during trophoblast differentiation for placentogenesis in cloned pregnancy. BMC Genomics 15, 43.
• Jakobsen J.E., Li J., Kragh P.M., Moldt B., Lin L., Liu Y., Schmidt M., Winther K.D., Schyth B.D., Holm I.E., Vajta G., Bolund L., Callesen H., Jřrgensen A.L.,Nielsen A.L., Mikkelsen J.G., 2011 – Pig transgenesis by Sleeping Beauty DNA transposition.Transgenic Research 20(3), 533-45.
• James R.M., Klerkx A.H., Keighren M., Flockhart J.H., West J.D., 1995 – Restricted distribution of tetraploid cells in mouse tetraploid <==> diploid chimeras. Developmental Biology 167(1), 213-226.
• Kaufman M.H., Webb S., 1990 – Postimplantation development of tetraploid mouse embryos produced by electrofusion. Development 110(4), 1121-1132.
• Kohan -Ghadr H.R., Smith L.C., Arnold D.R., Murphy B.D., Lefebvre R.C., 2012 – Aberrant expression of E-cadherin and β-carenin proteins in placenta of bovine embryos derived from somatic cell nuclear transfer. Reproduction in Fertility and Development 24(4), 588-98.
• Kragh P.M., Du Y., Corydon T.J., Purup S., Bolund L., Vajta G., 2005 – Efficient in vitro production of porcine blastocysts by handmade cloning with a combined electrical and chemical activation. Theriogenology 64(7), 1536-1545.
• Li J., Svarcova O., Villemoes K., Kragh P.M., Schmidt M., Břgh I.B., Zhang Y., Du Y., Lin L., Purup S., Xue Q., Bolund L., Yang H., Maddox-Hyttel P., Vajta G., 2009 – High in vitro development after somatic cell nuclear transfer and trichostatin A treatment of reconstructed porcine embryos.Theriogenology 70(5), 800-808.
• Mac Laren L.A., Anderson G.B., Bon Durant R.H., Edmondson A.J., 1993 – Reproductive cycles and pregnancy in interspecific sheep <==> goat chimaeras. Reproduction Fertility and Development 5(3), 261-70.
• Misica -Turner P.M., Oback F.C., Eichenlaub M., Wells D.N., Oback B., 2007 – Aggreating embryonic but not somatic nuclear transfer embryos increases cloning efficiency in cattle. Biology of Reproduction 76(2), 268-278.
• McLaren A., 1975 – Sex chimaerism and germ cell distribution in a series of chimaeric mice.Journal of Embryology & Experimental Morphology 33(1), 205-216.
• Nagy A., Rossant J., Nagy R., Abramow -Newerly W., Roder J.C., 1993 – Derivation of completely cell culture-derived mice from early-passage embryonic stem cells. Proceedingds of the National Academy of Sciences, USA 15, 90(18), 8424-8428.
• Ozil J.P., Modlinski J.A., 1986 – Effects of electric field on fusion rate and survival of 2-cell rabbit embryos. Journal of Embryology & Experimental Morphology 96, 211-28.
• Rorie R.W., Pool S.H., Prichard J.F., Betteridge K.J., Godke R.A., 1994 – A simplified procedure for making reconstituted blastocysts for interspecific and intergeneric transfer. Veterinary Record 135(8), 186-187.
• Schramm R.D., Paprocki A.M., 2004 – Strategies for the production of genetically identical monkeys by embryo splitting. Reproductive Biology and Endocrinology 2, 38.
• Sekirina G.G., Neganova I.E., 1995 – The microenvironment created by non-blocking embryos in aggregates may rescue blocking embryos via cell-embryo adherent contacts. Zygote 3(4),313-324.
• Shiue Y.L., Liou J.F., Shiau J.W., Yang J.R., Chen Y.H., Tailiu J.J., Chen L.R., 2006 – In vitro culture period but not the passage number influences the capacity of chimera production of inner cell mass and its deriving cells from porcine embryos. Animal Reproduction Science 93(1-2),134-143.
• Siriboon C., Tu C.F., Kere M., Liu M.S., Chang H.J., Ho L.L., Tai M.E., Fang W.D., Lo N.W., Tseng J.K., Ju J.C., 2014 – Production of viable cloned miniature pigs by aggregation of handmade cloned embryos at the 4-cell stage. Reproduction Fertility and Development 26(3), 395-406.
• Tang P.C., West J.D., 2000 – The effects of embryo stage and cell number on the composition of mouse aggregation chimaeras. Zygote 8(3), 235-243.
• Vajta G., Peura T.T., Holm P., Páldi A., Greve T., Trounson A.O., Callesen H.,2000 - New method for culture of zona-included or zona-free embryos: the Well of the Well (WOW) system. Molecular Reproduction and Development. 55(3), 256-264.
• Wells K.D., Powell A.M., 2000 – Blastomeres from somatic cell nuclear transfer embryos are not allocated randomly in chimeric blastocysts. Cloning 2(1), 9-22.
• West J.D., Flockhart J.H., Kissenpfennig A., 1995 – A maternal genetic effect on the composition of mouse aggregation chimaeras. Genetics Research 65(1), 29-40.
• Wood S.A., Pascoe W.S., Schmidt C., Kemler R., Evans M.J., Allen N.D., 1993 –Simple and efficient production of embryonic stem cell-embryo chimeras by coculture. Proceedingds of the National Academy of Sciences, USA 15 90(10), 4582-4585.
• Yoshioka K., Suzuki C., Tanaka A., Anas I.M., Iwamura S., 2002 – Birth of piglets derived from porcine zygotes cultured in chemically defined medium. Biology of Reproduction 66(1), 112-119.