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2017 | 66 | 4 |
Tytuł artykułu

Temperature, pH and trimethoprim-sulfamethoxazole are potent inhibitors of biofilm formation by Stenotrophomonas maltophilia clinical isolates

Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Stenotrophomonas maltophilia, an opportunistic pathogen usually connected with healthcare-associated infections, is an environmental bacterium. Intrinsic resistance to multiple antibiotics, with different virulence determinants in the last decade classified this bacterium in the group of global multiple drug resistant (MDR) organism. S. maltophilia clinical isolates, were collected from tertiary care pediatric hospital in Belgrade, Serbia to investigate influence of different factors on biofilm formation, kinetics of biofilm formation for strong biofilm producers and effect of trimethoprim-sulfamethoxazole (TMP/SMX) on formed biofilm. Most of the isolates (89.8%) were able to form a biofilm. Analysis of biofilm formation in different growth conditions showed that changing of temeperature and pH had the stronggest effect on biofilm formation almost equally in group of cystic fibrosis (CF) and non-CF strains. TMP/SMX in concentration of 50 μg/ml reduced completely 24 h old biofilms while concentration of 25 μg/ml effects formed biofilms in a strain dependent manner. Among strains able to form strong biofilm CF isolates formed biofilm slower than non-CF isolates, while shaking conditions did not affect biofilm formation. Swimming motility was detected in both CF and non-CF isolates, however more motile strain formed stronger biofilms. This study suggests that temperature, pH and TMP/SMX had the strongest influence on biofilm formation in analyzed collection of S. maltophilia. A positive correlation between motility and strength of formed biofilm was demonstrated.
Słowa kluczowe
EN
Wydawca
-
Rocznik
Tom
66
Numer
4
Opis fizyczny
p.433-38,fig.,ref.
Twórcy
autor
  • Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia
  • Faculty of Biology, University of Belgrade, Belgrade, Serbia
  • Laboratory of Systems Biology and Genetics, Institute for Bioengineering, School of Life Sciences, Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland
autor
  • Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia
  • Institute for Mother and Child Health Care of Serbia “Dr Vukan Cupic”, Belgrade, Serbia
autor
  • Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia
autor
  • Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia
  • Faculty of Biology, University of Belgrade, Belgrade, Serbia
autor
  • Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia
  • Faculty of Biology, University of Belgrade, Belgrade, Serbia
Bibliografia
  • Abbott I.J., M.A. Slavin, J.D. Turnidge, K.A. Thursky and L.J. Worth. 2011. Stenotrophomonas maltophilia: emerging disease patterns and challenges for treatment. Expert. Rev. Anti Infect. Ther. 9: 471–488.
  • Balcázar J.L., J. Subirats and C.M. Borrego. 2015. The role of biofilms as environmental reservoirs of antibiotic resistance. Front. Microbiol. 6: 1216.
  • Brooke J.S. 2012. Stenotrophomonas maltophilia: an emerging global opportunistic pathogen. Clin. Microbiol. Rev. 25: 2–41.
  • Brooke J.S. 2014. New strategies against Stenotrophomonas maltophilia: a serious worldwide intrinsically drag-resistant opportunistic pathogen. Expert. Rev. Anti Infect. Ther. 12: 1–4.
  • Cantón R. and del R. Campo. 2010. Cystic fibrosis: deciphering the complexity. Clin. Microbiol. Infect. 16: 793–797.
  • Di Bonaventura G., S. Stepanović, C. Picciani, A. Pompilio and R. Piccolomini. 2007. Effect of environmental factors on biofilm formation by clinical Stenotrophomonas maltophilia isolates. Folia Microbiol. 52: 86–90.
  • Di Bonaventura G., R. Piccolomini, D. Paludi, D. D’Orio, A. Vergara, M. Conter M, and A. Ianieri. 2008. Influence of temporature on biofilm formation by Listeria monocytogenes on various food-contact surfaces: relationship with motility and cell surface hydrophobicity. J. Appl. Microbiol. 104: 1552–1561.
  • Hu L.F., G.S. Chen, Q.X. Kong, L.P. Gao, X. Chen, Y. Ye and J.B. Li. 2016. Increase in the prevalence of resistance determinants to trimethoprim/sulfamethoxazole in Clinical Stenotrophomonas maltophilia Isolates in China. PLOS One 11: e0157693.
  • Madi H., J. Lukić, Z. Vasiljević, M. Biočanin, M. Kojić, B. Jovčić and J. Lozo. 2016. Genotypic and phenotypic characterization of Stenotrophomonas maltophilia strains from a pedriatric tertiary care hospital in Serbia. PloS ONE 11: e0165660.
  • Payne D.E. and B.R. Boles. 2015. Emerging interactions between ma-trix components during biofilm development. Curr. Genet. 62: 137–141.
  • Pompilio A., R. Piccolomini, C. Picciani, D. D’Antonio, V. Savini and G. di Bonaventura. 2008. Factors associated with adherence to and biofilm formation on polystyrene by Stenotrophomonas maltophilia: the role of cell surface hydrophobicity and motility. FEMS Microbiol. Lett. 287: 41–47.
  • Pompilio A., S. Pomponio, V. Crocetta, G. Gherardi, F. Verginelli, E. Fiscarelli, G. Dicuonzo, V. Savini, D. D’Antonio andG. di Bonaventura. 2011. Phenotypic and genotypic characterization of Stenotrophomonas maltophilia isolates from patients with cystic fibrosis: genome diversity, biofilm formation, and virulence. BMC Microbiol. 5, 11: 159.
  • Pompilio A., V. Crocetta, S. De Nicola, F. Verginelli, E. Fiscarelli and G. di Bonaventura. 2015. Cooperative pathogenicity in cystic fibrosis: Stenotrophomonas maltophilia modulates Pseudomonas aeruginosa virulence in mixed biofilm. Front. Microbiol. 6: 951.
  • Sanders D.B., R.C. Bittner, M. Rosenfeld, L.R. Hoffman, G.J. Redding and C.H. Goss. 2010. Failure to recover to baseline pulmonary function after cystic fibrosis pulmonary exacerbation. Am. J. Respir. Crit. Care. Med. 182: 627–632.
  • Sánchez M.B. 2015. Antibiotic resistance in the opportunistic pathogen Stenotrophomonas maltophilia. Front. Microbiol. 6: 658.
  • Stepanović S., D. Vuković, V. Hola, G. Di Bonaventura, S. Djukić, I. Ćirković and F. Ruzicka. 2007. Quantification of biofilm in microtiter plates: overview of testing conditions and practical recommendations for assessment of biofilm production by staphylococci. APMIS 115: 891–899.
  • The A.H., S.M. Lee and G.A. Dykes. 2016. The influence of prior modes of growth, temperature, medium, and substrate surface on biofilm formation by antibiotic-resistant Campylobacter jejuni. Curr. Microbiol. 73: 859–866.
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
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