Molecular identification of cryptic species of Ceratomyxa Thelohan, 1892 (Myxosporea: Bivalvulida) including the description of eight novel species from apogonid fishes (Perciformes: Apogonidae) from Australian waters

• Autorzy: Heiniger H. , Adlard R. D.
• Języki publikacji: EN

Abstrakty

EN

Ceratomyxa parasites from the gall bladders of 23 species of cardinalfishes (family Apogonidae) from Australian waters were examined for their taxonomic identity and phylogenetic relatedness. We identified 15 of the 23 apogonid fish species infected with species of Ceratomyxa. Although the majority of apogonid species harboured only a single Ceratomyxa species, four were found with multiple species of Ceratomyxa. This study describes eight novel species using a combination of morphological, small subunit ribosomal DNA (SSU rDNA) and biological characters. Six Ceratomyxa species are reported from single apogonid species, while two are reported from multiple host species. Molecular data were critical in identifying several morphologically cryptic species. However, our results suggest that SSU rDNA was not capable of distinguishing all the species present in the current study system and alternative genetic markers should be investigated in the future.

Słowa kluczowe

EN

Ceratomyxa; Myxosporea; Bivalvulida; Apogonidae; cryptic species; host specificity; phylogenesis; diversity; parasite; Australian water

• Wydawca: -
• Czasopismo: Acta Parasitologica
• Rocznik: 2013
• Tom: 58
• Numer: 3
• Opis fizyczny: p.342-360,fig.,ref.

Twórcy

autor

Heiniger H.

• Natural Environments Program, Queensland Museum, PO Box 3300, South Brisbane, Queensland 4101, Australia

autor

Adlard R. D.

• Natural Environments Program, Queensland Museum, PO Box 3300, South Brisbane, Queensland 4101, Australia

Bibliografia

• Alama-Bermejo G., Raga J.A., Holzer A.S. 2011. Host-parasite relationship of Ceratomyxa puntazzi n. sp. (Myxozoa: Myxosporea) and sharpsnout seabream Diplodus puntazzo (Walbaum, 1792) from the Mediterranean with first data on ceratomyxid host specificity in sparids. Veterinary Parasitology, 182, 181–192. DOI: 10.1016/j.vetpar.2011.05.012.
• Allen G.R. 1993. Cardinalfishes (Apogonidae) of Madang Province, Papua New Guinea, with descriptions of three new species. Revue Francaise d’Aquariologie, 20, 9–20.
• Bartošováclav H. 2009. Concatenated SSU and LSU rDNA data confirm the main evolutionary trends within myxosporeans (Myxozoa: Myxosporea) and provide an effective tool for their molecular phylogenetics. Molecular Phylogenetics and Evolution, 53, 81–93. DOI: 10.1016/j.ympev.2009.05.018.
• Burger M.A.A., Adlard R.D. 2010. Four new species of Kudoa Meglitsch, 1947 (Myxosporea: Multivalvulida) from Australia with recommendations for species descriptions in the Kudoidae. Parasitology, 137, 793–814. DOI: 10.1017/S00311 82009991557.
• Drummond A.J., Ashton B., Buxton S., Cheung M., Cooper A., Duran C., Field M., Heled J., Kearse M., Markowitz S., Moir R., Stones-Havas S., Sturrock S., Thierer T., Wilso A. 2010. Geneious v 5.6, Available from http://www.geneious.com.
• Edgar R.C. 2004. MUSCLE: a multiple sequence alignment method with reduced time and space complexity. BMC Bioinformatics, 5, 113. DOI: 10.1186/1471-2105-5-113.
• Eiras J.C. 2006. Synopsis of the species of Ceratomyxa Thélohan, 1892 (Myxozoa: Myxosporea: Ceratomyxidae). Systematic Parasitology, 65, 49–71. DOI: 10.1007/s11230-006-9039-5.
• Fiala I. 2006. The Phylogeny of the Myxosporea (Myxozoa) based on small subunit ribosomal RNA gene analysis. International Journal for Parasitology, 36, 1521–1534. DOI: 10.1016/j.ijpara. 2006.06.016.
• Gleeson R.J., Adlard R.D. 2011. Morphological and genetic analysis of three new species of Ceratomyxa Thélohan, 1892 (Myxozoa: Myxosporea) from carcharhinid sharks off Australia. Systematic Parasitology, 80, 117–124. DOI: 10.1007/s11230-011-9316-9.
• Greenfield D.W., Johnson R.K. 1990. Heterogeneity in habitat choice in Cardinalfish community structure. Copeia, 4, 1107–1114. DOI: 10.2307/1446495.
• Gunter N.L. 2009. Relatedness, host specificity and richness of the genus Ceratomyxa (Myxozoa: Myxosporea) in teleost fishes. PhD Thesis, The University of Queensland, Brisbane, Australia.
• Gunter N.L., Adlard R.D. 2008. Bivalvulidan (Myxozoa: Myxosporea) parasites of damselfishes with description of twelve novel species from Australia’s Great Barrier Reef. Parasitology, 135, 1165–1178. DOI: 10.1017/S0031182008 004733.
• Gunter N.L., Adlard R.D. 2009. Seven new species of Ceratomyxa Thelohan, 1892 (Myxozoa) from the gall-bladders of serranid fishes from the Great Barrier Reef, Australia. Systematic Parasitology, 73, 1–11. DOI: 10.1007/s11230-008-9162-6.
• Gunter N.L., Adlard R.D. 2010. The demise of Leptotheca Thélohan, 1895 (Myxozoa: Myxosporea: Ceratomyxidae) and assignment of its species to Ceratomyxa Thélohan, 1892 (Myxosporea: Ceratomyxidae), Ellipsomyxa Køie, 2003 (Myxosporea: Ceratomyxidae), Myxobolus Bütschli, 1882 and Sphaerospora Thélohan, 1892 (Myxosporea: Sphaerosporidae). Systematic Parasitology, 75, 81–104. DOI: 10.1007/s11230-009-9227-1.
• Gunter N.L., Burger M.A.A., Adlard R.D. 2010. Morphometric and molecular characterisation of four new Ceratomyxa species (Myxosporea: Bivalvulida: Ceratomyxidae) from fishes off Lizard Island, Australia. Folia Parasitologica, 57, 1–10.
• Gunter N.L., Whipps C.M., Adlard R.D. 2009. Ceratomyxa (Myxozoa: Bivalvulida): Robust taxon or genus of convenience? International Journal for Parasitology, 39, 1395–1405. DOI: 10.1016/j.ijpara.2009.04.008.
• Hammer O., Harper D.A.T., Ryan P.D. 2001. PAST: Paleontological Statistics software package for education and data analysis. Palaeontologia Electronica, 4, 9 pp.
• Heiniger H., Gunter N.L., Adlard R.D. 2008. Relationships between four novel ceratomyxid parasites from the gall bladders of labrid fishes from Heron Island, Australia. Parasitology International, 57, 158–165. DOI: 10.1016/j.parint.2007.11.006.
• Kuiter R.H., Kozawa T. (Eds.). 1999. Fishes of the Indo-West Pacific: Apogonidae (2nd ed.). Victoria, Australia.
• Lom J., Arthur J.R. 1989. A guidline for the preparation of species descriptions in Myxosporea. Journal of Fish Diseases, 12, 151–156. DOI: 10.1111/j.1365-2761.1989.tb00287.x
• Lom J., Dykova I. 2006. Myxozoan genera: definition and notes on taxonomy, life cycle terminology and pathogenic species. Folia Parasitologica, 53, 1–36.
• Maddison D.R., Maddison W.P. 2005. MacClade 4: Analysis of phylogeny and character evolution. Version 4.08. Sunderland, Massachusetts: Sinauer Associates.
• Meglitsch P. A. 1960. Some coelozoic Myxosporidia from New Zealand fishes I. — General, and Family Ceratomyxidae. Transactions of the Royal Society of New Zealand, 88, 265–356.
• Miller M.A., Pfeiffer W., Schwartz T. 2010. Creating the CIPRES Science Gateway for inference of large phylogenetic trees. In: Proceedings of the Gateway Computing Environments Workshop (CGE), 14 Nov. 2010, New Orelean, LA, 1–8. DOI: 10.1109/GCE.2010.5676129.
• Moser M., Kent M.L., Dennis D. 1989. Gall-bladder Myxosporea in coral-reef fishes from Heron Island, Australia. Australian Journal of Zoology, 37, 1–13. DOI: 10.1071/ZO9890001.
• Narasimhamurti C.C., Kalavati C., Anuradha I., Dorothy K.P. 1990. Studies on protozoan parasites of deep water fishes from the Bay of Bengal. In: Proceedings of the First Workshop of Scientific Results of FORV Sagar Sampada, 5–7 June 1989, Cochin, India, 325–336.
• Posada D. 2008. jModelTest: Phylogenetic Model Averaging. Molecular Biology and Evolution, 25, 1253–1256. DOI: 10.1093/molbev/msn083.
• Ronquist F., Huelsenbeck J.P. 2003. MRBAYES 3: Bayesian phylogenetic inference under mixed models. Bioinformatics, 19, 1572–1574. DOI: 10.1093/bioinformatics/btg180.
• Sitja-Bobadilla A., Alvarez-Pellitero P. 1993. Light and electronmicroscopic description of Ceratomyxa labracis n. sp. and a redescription of C. diplodae (Myxosporea, Bivalvulida) from wild and cultered Mediterranean Sea Bass Dicentrarchus labrax (L.) (Teleostei, Serranidae). Systematic Parasitology, 26, 215–223. DOI: 10.1007/BF00009729.
• Stamatakis A., Hoover P., Rougemont J. 2008. A rapid bootstrapping algorithm for the RAxML web-servers. Systematic Biology, 57, 758–771. DOI: 10.1080/10635150802429642.
• Swofford D.L. 2002. PAUP*. Phylogenetic analysis using parsimony (*and other methods). Sunderland, MA: Sinauer Associates.
• Thompson J.D., Higgins D.G., Gibson T.J. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research, 22, 4673–4680. DOI: 10.1093/nar/22.22.4673.
• Whipps C.M., Adlard R.D., Bryant M.S., Lester R.J.G., Findlay V., Kent M.L. 2003. The first report of three Kudoa species from Eastern Australia: Kudoa thyrsites from Mahi mahi (Coryphaena hippurus), Kudoa amamiensis and Kudoa minithyrsites n. sp. from Sweeper (Pempheris ypsilychnus). Journal of Eukaryotic Microbiology, 50, 215–219. DOI: 10.1111/j.1550-7408.2003.tb00120.x.
• Whipps C.M., Grossel G., Adlard R.D., Yokoyama H., Bryant M.S., Munday B.L., Kent M. L. 2004. Phylogeny of the Multivalvulida (Myxozoa: Myxosporea) based on comparative ribosomal DNA sequence analysis. Journal of Parasitology, 90, 618–622. DOI: 10.1645/GE-153R.