The transition of proembryogenic masses to somatic embryos in Araucaria angustifolia (Bertol.) Kuntze is related to the endogenous contents of IAA, ABA and polyamines

• Autorzy: Farias-Soares F.L. , Steiner N. , Schmidt E.C. , Pereira M.L.T. , Rogge-Renner G.D. , Bouzon Z.L. , Floh E.S.I. , Guerra M.P.
• Języki publikacji: EN

Abstrakty

EN

In somatic embryogenesis (SE) of conifers, the inability of many embryogenic cell lines to form well-developed somatic embryos may results from failure and constraints during the transition of proembryogenic masses (PEMs) to early somatic embryos. In the present work, we propose the inclusion of a preculture and prematuration steps looking at enhancing PEM III-to-early somatic embryos transition. It was further hypothesized that these results would correlate with the contents of endogenous indole-3-acetic acid (IAA), abscisic acid (ABA) and polyamines (PA). To test these hypotheses, the embryogenic culture was subjected to preculture with fluridone (FLD) and prematuration treatments with different combinations of carbon source and polyethylene glycol (PEG). The frequency of PEM III was increased after FLD preculture and the contents of IAA and ABA decreased, while the contents of PA increased. Putrescine (Put) was the most abundant PA present at this stage, followed by spermidine (Spd) and spermine (Spm). In early embryogenesis, prematuration treatments supplemented with maltose or lactose plus PEG enhanced the PEM III-to-early somatic embryos transition. IAA and ABA contents increased at this stage, while a decrease of the total free PA levels was observed. Put was the most abundant PA, followed by Spd and Spm, mainly in the treatment supplemented with PEG. This resulted in a decrease of PA ratio (Put/Spd + Spm) and, hence, PEM III-to-early somatic embryos transition. It was concluded that the preculture with FLD and prematuration treatments promote the PEM III-to-early somatic embryos transition throughout the whole early developmental process in Araucaria angustifolia.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2014
• Tom: 36
• Numer: 07
• Opis fizyczny: p.1853-1865,fig.,ref.

Twórcy

autor

Farias-Soares F.L.

• Graduate Program in Plant Genetic Resources, Department of Plant Science, Federal University of Santa Catarina, Florianopolis, SC C.P. 476, 88040-900, Brazil

autor

Steiner N.

• Department of Botany, Plant Physiology Laboratory, Federal University of Santa Catarina, Florianopolis, SC 88040-900, Brazil

autor

Schmidt E.C.

• Department of Cell Biology, Embryology and Genetics, Plant Cell Biology Laboratory, Federal University of Santa Catarina, Florianopolis, SC C.P. 476, 88049-900, Brazil

autor

Pereira M.L.T.

• Laboratory of Plant, Department of Plant Science, Development Physiology and Genetics, Federal University of Santa Catarina, Florianopolis, SC C.P. 476, 88040-900, Brazil

autor

Rogge-Renner G.D.

• Department of Cell Biology, Embryology and Genetics, Plant Cell Biology Laboratory, Federal University of Santa Catarina, Florianopolis, SC C.P. 476, 88049-900, Brazil

autor

Bouzon Z.L.

• Central Laboratory of Electron Microscopy, Federal University of Santa Catarina, Florianopolis, SC, Brazil

autor

Floh E.S.I.

• Plant Cell Biology Laboratory, Department of Botany, Institute of Biosciences (IB), University of Sao Paulo (USP), Sao Paulo, SP 05422-970, Brazil

autor

Guerra M.P.

• Laboratory of Plant, Department of Plant Science, Development Physiology and Genetics, Federal University of Santa Catarina, Florianopolis, SC C.P. 476, 88040-900, Brazil

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Identyfikatory

DOI

10.1007/s11738-014-1560-6