Photothrombotic stroke induces long-lasting changes in mRNA expression of enzymes involved in polysaccharide metabolism in the brain areas remote to infarct

• Autorzy: Greda A.K. , Nowicka D.
• Języki publikacji: EN

Abstrakty

EN

INTRODUCTION: Perineuronal nets (PNNs) are brain extracellular matrix (ECM) formations enveloping selected neurons. They stabilize synaptic connections, thus limiting plasticity. Decrease in PNNs densities was observed after stroke and may be considered an attempt to create favorable conditions for neuroplasticity. PNNs are composed mainly of sugar moieties, therefore we hypothesize the role of polysaccharide modifying enzymes in observed phenomenon. AIM(S): We investigated the changes in gene expression and protein localization of enzymes involved in hyaluronic acid and chondroitin sulfate metabolism. METHOD(S): The expression of genes was analyzed in the remote area at 7 days (d), 1 and 3 months (m) after unilateral photothrombosis. To investigate spatiotemporal mRNA expression qPCR method was employed. Immunohistochemical staining was used to analyze cellular localization of investigated enzymes. RESULTS: We observed increased expression of hyaluronan synthase 2 (HAS2) 7d post-stroke within both hemispheres. Also, changes in mRNA level of chondroitin sulfate synthesis-mediating enzymes occurred, namely, elevation of beta-1,3-glucuronyltransferase 2 (B3GAT2) and simultaneous decrease in B3GAT1 and B3GAT3 expression. Progressive reduction of chondroitin sulfate N-acetylgalactosaminyltransferase 1 (ChGn-1) expression was observed from 7d to 1 month in the contralateral homotopic area. Moreover, 7d after stroke decrease in chondroitin-4-sulfotranseferase 1 (C4ST1) accompanied by increase in arylsulfatase B (ARSB) mRNA level was observed. Importantly, protein of investigated enzymes, which mRNA level alteration occurred at shorter time point, were still observed 1m after stroke. CONCLUSIONS: Obtained data indicate prolonged time window for modification of polysaccharide components of the brain ECM that may affect rearrangement of synaptic connections after stroke. FINANCIAL SUPPORT: This work was supported by National Science Centre (Poland) grants: 2012/05/B/ NZ3/00851 and 2015/17/N/NZ3/02244.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2017
• Tom: 77
• Numer: Suppl.1
• Opis fizyczny: p.58

Twórcy

autor

Greda A.K.

• Laboratory of Epileptogenesis, Department of Molecular and Cellular Neurobiology, Nencki Institute of Experimental Biology Polish Academy of Sciences, Warsaw, Poland

autor

Nowicka D.

• Laboratory of Epileptogenesis, Department of Molecular and Cellular Neurobiology, Nencki Institute of Experimental Biology Polish Academy of Sciences, Warsaw, Poland