Antibacterial activity of Tribulus terrestris methanol extract against clinical isolates of Escherichia coli

• Autorzy: Batoei S. , Mahboubi M. , Yari R.

Warianty tytułu

PL

Działanie antybakteryjne wyciągu etanolowego z Tribulus terrestris na kliniczne izolaty Escherichia coli

• Języki publikacji: EN

Abstrakty

EN

Introduction: Tribulus terrestris L. is traditionally used for treatment of urinary tract infections. Escherichia coli, as the most prominent agent of urinary tract infections, can be sensitive to T. terrestris extract. Objectives: The aim of this study was to evaluate the antibacterial activity of T. terrestris methanol extract against clinical isolates of E. coli from urinary tract infections. Saponins were determined as main constituents of T. terrestris methanol extract. Methods: The antibacterial activities of T. terrestris methanol extract were evaluated by micro-broth dilution assay. The synergistic effects of T. terrestris methanol extract were screened with gentamicin by micro titer plate and disc diffusion assays. The isobologram curve was figured and the Fractional Inhibitory Concentration Index (FICI) was determined. Results: The saponin content of T. terrestris methanol extract was 54% (w/w). The means of MIC and MBC values for E. coli clinical isolates (n=51) were 3.5±0.27 and 7.4±0.5 mg/ml while these amounts were 3.9±1.3 and 6.4±1.8 μg/ml for gentamicin. T. terrestris methanol extract and gentamicin had synergistic effect with FICI equal to 0.1375. Conclusion: Therefore, T. terrestris can be applicable as alternative treatment in management of urinary tract infections.

PL

Wstęp: Tribulus terrestris L. jest tradycyjnie stosowany w leczeniu zakażeń układu moczowego. Escherichia coli, jako główny czynnik zakażeń tego układu, może wykazywać wrażliwość na działanie ekstraktu z T. terrestris.Cel: Celem badań było określenie aktywności przeciwbakteryjnej ekstraktu metanolowego uzyskanego z ziela T. terrestris w stosunku do szczepów E. coli izolowanych od osób z zakażeniami układu moczowego. Metody: Działanie przeciwbakteryjne ekstraktu metanolowego z T. terrestris określano metodą rozcieńczeń w pożywce płynnej. Działanie synergistyczne ekstraktu metanolowego z T. terrestris z gentamycyną badano metodą krążków bibułowych w podłożu agarowym. Na tej podstawie wyznaczono krzywą izobolograficzną oraz ułamkowe stężenie hamujace dla obu substancji (Fractional Inhibitory Concentration Index – FICI). Wyniki: Zawartość saponin w ekstrakcie metanolowym z T. terrestris wynosiła 54% (w/w). Średnie wartości MIC i MBC dla szczepów klinicznych E. coli (n=51) wynosiły odpowiednio 3,5±0,27, 7,4±0,5 mg/ml, a dla gentamycyny odpowiednio 3,9±1,3 i 6,4±1,8 µg/ml. Ekstrakt metanolowy i gentamycyna wykazywały działanie synergistyczne na poziomie FICI równego 0,1375. Wniosek: Wykazano, że ekstrakt metanolowy z ziela T. terrestris, może być stosowany jako alternatywny środek do leczenia zakażeń układu moczowego.

Słowa kluczowe

EN

Tribulus terrestris; methanol extract; synergy; gentamicin; saponin; Escherichia coli; clinical isolate

• Wydawca: -
• Czasopismo: Herba Polonica
• Rocznik: 2016
• Tom: 62
• Numer: 2
• Opis fizyczny: p.57-66,fig.,ref.

Twórcy

autor

Batoei S.

• Biology Department, Faculty of Sciences, Borujerd Branch Islamic Azad University, Borujerd, Iran

autor

Mahboubi M.

• Microbiology Department, Medicinal Plant Research, Center of Barij, Kashan, Iran

autor

Yari R.

• Biology Department, Faculty of Sciences, Borujerd Branch Islamic Azad University, Borujerd, Iran

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Identyfikatory

DOI

10.1515/hepo-2016-0011