EN
The described method is based on acetone reaction with salicylaldehyde and is applied for the determination of acetone in milk. In this aim 1 ml of milk is added to 3 ml of the reactive mixture (potassium hydroxide plus concentrated salicylaldehyde) and left at room temperature for 90 min. The intensity of orange colour corresponds to the level of acetone in milk. The concentration of acetone in milk samples with negative reactions was 42 umol/1, while positive reactions corresponded to 149, 370 and 1169 umol/1 respectively. By means of the test 2910 samples of milk were examined of which 179 showed positive reactions. In this group 67% was evaluated clinically as suspected ketosis (+), 22% as subclinicail ketosis (++) and 11% as a clinical form of ketosis (+ + + ). The test under study appeared to be useful as a screening test and should be applied in practice.