PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2018 | 40 | 03 |
Tytuł artykułu

Cloning and expression of a new cytoplasmic small heat shock protein gene from Pinellia ternata

Autorzy
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
A new cytoplasmic small heat shock protein gene was cloned from Pinellia ternata (Thunb.) Breit and designated PtHSP18.2 (GenBank number: KF693218). The PtHSP18.2 gene has an open reading frame of 711 bp that encodes 159 amino acids, and a molecular mass of 18.20 kDa. The phylogenetic tree indicated high homology of the PtHSP18.2 protein with that of cytosolic class I small heat shock proteins (CI sHSPs) from other plant species. PtHSP18.2 is expressed in root, leaf, and stem tissues. The expression of PtHSP18.2 was increased under heat and cold stress but was not induced by drought stress. When overexpressed in E. coli, PtHSP18.2 was correlated with the preservation of viability under heat stress. The CI sHSP family is the largest sHSP subfamily, and several different members of this subfamily are present in P. ternata. Two-dimensional electrophoresis (2-DE) western blot analysis with an anti-AtHSP17.7 (a member of CI sHSPs) antibody revealed five 18 kDa heat shock-induced P. ternata proteins. In combination, our findings suggest that PtHSP18.2 is potentially significant in the response to thermal stress.
Słowa kluczowe
EN
Wydawca
-
Rocznik
Tom
40
Numer
03
Opis fizyczny
Article 44 [10p.], fig.,ref.
Twórcy
autor
  • Institute of Chinese Medicinal Materials, Nanjing Agricultural University, Nanjing 210095, China
  • School of Pharmacy, Henan University of Traditional Chinese Medicine, Zhengzhou, Henan, China
autor
  • Institute of Modern Chinese Medical Materials, Guizhou Academy of Agricultural Sciences, Guiyang 550006, China
autor
  • Institute of Chinese Medicinal Materials, Nanjing Agricultural University, Nanjing 210095, China
autor
  • Institute of Chinese Medicinal Materials, Nanjing Agricultural University, Nanjing 210095, China
autor
  • Institute of Chinese Medicinal Materials, Nanjing Agricultural University, Nanjing 210095, China
autor
  • Institute of Modern Chinese Medical Materials, Guizhou Academy of Agricultural Sciences, Guiyang 550006, China
Bibliografia
  • Al-Whaibi MH (2011) Plant heat-shock proteins: a mini review. J King Saud Univ Sci 23:139–150
  • Bondino HG, Valle EM, Ten HA (2012) Evolution and functional diversification of the small heat shock protein/alpha-crystallin family in higher plants. Planta 235:1299–1313
  • Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
  • Carranco R, Almoguera C, Jordano J (1997) A plant small heat shock protein gene expressed during zygotic embryogenesis but noninducible by heat stress. J Biol Chem 272:27470–27475
  • Chung HS, Um JY, Kim MS, Hong SH, Kim SM, Kim HK, Park SJ, Kim SC, Hwang WJ, Kim HM (2002) Determination of the site of origin of Pinellia ternata roots based on RAPD analysis and PCR-RFLP. Hereditas 136:126–129
  • Florentin A, Damri M, Grafi G (2013) Stress induces plant somatic cells to acquire some features of stem cells accompanied by selective chromatin reorganization. Dev Dyn 10:1121–1133
  • Guan J, Jinn T, Yeh C, Feng S, Chen Y, Lin C (2004) Characterization of the genomic structures and selective expression profiles of nine class I small heat shock protein genes clustered on two chromosomes in rice (Oryza sativa L.). Plant Mol Biol 56:795–809
  • Jiang C, Xu J, Zhang H, Zhang X, Shi J, Li M, Ming F (2009) A cytosolic class I small heat shock protein, RcHSP17. 8, of Rosa chinensis confers resistance to a variety of stresses to Escherichia coli, yeast and Arabidopsis thaliana. Plant Cell Environ 32:1046–1059
  • Kobayashi M, Katoh H, Takayanagi T, Suzuki S (2010) Characterization of thermotolerance-related genes in grapevine (Vitis vinifera). J Plant Physiol 167:812–819
  • Kotak S, Larkindale J, Lee U, von Koskull-Döring P, Vierling E, Scharf K (2007) Complexity of the heat stress response in plants. Curr Opin Plant Biol 10:310–316
  • Lu H, Xue T, Zhang A, Sheng W, Zhu Y, Chang L, Song Y, Xue J (2013) Construction of an SSH library of Pinellia ternata under heat stress, and expression analysis of four transcripts. Plant Mol Biol Rep 31:185–194
  • Lujan R, Lledias F, Martinez LM, Barreto R, Cassab GI, Nieto-Sotelo J (2009) Small heat-shock proteins and leaf cooling capacity account for the unusual heat tolerance of the central spike leaves in Agave tequilana var. Weber. Plant Cell Environ 32:1791–1803
  • Mittler R (2006) Abiotic stress, the field environment and stress combination. Trends Plant Sci 11:15–19
  • Nover L, Bharti K, Doring P, Mishra SK, Ganguli A, Scharf KD (2001) Arabidopsis and the heat stress transcription factor world: how many heat stress transcription factors do we need? Cell Stress Chaperones 6:177–189
  • Sarkar NK, Kim YK, Grover A (2009) Rice sHsp genes: genomic organization and expression profiling under stress and development. BMC Genom 10:393
  • Sato Y, Yokoya S (2008) Enhanced tolerance to drought stress in transgenic rice plants overexpressing a small heat-shock protein, sHSP17.7. Plant Cell Rep 27:329–334
  • Scharf KD, Siddique M, Vierling E (2001) The expanding family of Arabidopsis thaliana small heat stress proteins and a new family of proteins containing alpha-crystallin domains (Acd proteins). Cell Stress Chaperones 6:225–237
  • Siddique M, Gernhard S, von Koskull-Doring P, Vierling E, Scharf KD (2008) The plant sHSP superfamily: five new members in Arabidopsis thaliana with unexpected properties. Cell Stress Chaperones 13:183–197
  • Simoes-Araujo JL, Alves-Ferreira M, Rumjanek NG, Margis-Pinheiro M (2008) VuNIP1 (NOD26-like) and VuHSP17.7 gene expression are regulated in response to heat stress in cowpea nodule. Environ Exp Bot 63:256–265
  • Song N, Ahn Y (2011) DcHsp17.7, a small heat shock protein in carrot, is tissue-specifically expressed under salt stress and confers tolerance to salinity. New Biotechnol 28:698–704
  • Soto A et al (1999) Heterologous expression of a plant small heat-shock protein enhances Escherichia coli viability under heat and cold stress. Plant Physiol 120(2):521–528
  • Sun W, Van Montagu M, Verbruggen N (2002) Small heat shock proteins and stress tolerance in plants. Biochim Biophys Acta (BBA) Gene Struct Expr 1577:1–9
  • Sun L, Liu Y, Kong X, Zhang D, Pan J, Zhou Y, Wang L, Li D, Yang X (2012) ZmHSP16.9, a cytosolic class I small heat shock protein in maize (Zea mays), confers heat tolerance in transgenic tobacco. Plant Cell Rep 31:1473–1484
  • Tao P, Liu L, Wang JB (2012) Characterization of eight cytosolic sHSP genes and their expression in Capsella bursa-pastoris. Biol Plant 56:648–656
  • Volkov RA, Panchuk II, Schoffl F (2005) Small heat shock proteins are differentially regulated during pollen development and following heat stress in tobacco. Plant Mol Biol 57:487–502
  • Wan M, Chen H, Zhan Y, Zhou J (1995) Tissue culture and rapid propagation of Pinellia ternata (Thunb) Breit. Zhongguo Zhong Yao Za Zhi 20(526–529):574
  • Wang W, Vignani R, Scali M, Cresti M (2006) A universal and rapid protocol for protein extraction from recalcitrant plant tissues for proteomic analysis. Electrophoresis 27:2782–2786
  • Waters ER, Vierling E (1999) The diversification of plant cytosolic small heat shock proteins preceded the divergence of mosses. Mol Biol Evol 16:127–139
  • Waters ER, Lee GJ, Vierling E (1996a) Evolution, structure and function of the small heat shock proteins in plants. J Exp Bot 47:325–338
  • Waters ER, Lee GJ, Vierling E (1996b) Duplication of the class I cytosolic small heat shock protein gene and potential functional divergence revealed by sequence variations flanking the a-crystallin. J Exp Bot 47:325–338
  • Wehmeyer N, Vierling E (2000) The expression of small heat shock proteins in seeds responds to discrete developmental signals and suggests a general protective role in desiccation tolerance. Plant Physiol 122:1099–1108
  • Zhang M, Li G, Huang W, Bi T, Chen G, Tang Z, Su W, Sun W (2010) Proteomic study of Carissa spinarum in response to combined heat and drought stress. Proteomics 10:3117–3129
  • Zhu Y, Zhu G, Guo Q, Zhu Z, Wang C, Liu Z (2013) A comparative proteomic analysis of Pinellia ternata leaves exposed to heat stress. Int J Mol Sci 14:20614–20634
  • Zou J, Liu A, Chen X, Zhou X, Gao G, Wang W, Zhang X (2009) Expression analysis of nine rice heat shock protein genes under abiotic stresses and ABA treatmen. J Plant Physiol 8:851–861
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-241a7425-d123-4152-9cc1-c54fe830f671
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.