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2013 | 60 | 4 |
Tytuł artykułu

Generation of stable, non-aggregating Saccharomyces cerevisiae wild isolates

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Cellular aggregates observed during growth of Saccharomyces cerevisiae strains derived from various natural environments makes most laboratory techniques optimized for non-aggregating laboratory strains inappropriate. We describe a method to reduce the size and percentage of the aggregates. This is achieved by replacing the native allele of the AMN1 gene with an allele found in the W303 laboratory strain. The reduction in aggregates is consistent across various environments and generations, with no change in maximum population density or strain viability, and only minor changes in maximum growth rate and colony morphology.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
60
Numer
4
Opis fizyczny
p.657-660,fig.,ref.
Twórcy
  • Institute of Environmental Sciences, Jagiellonian University, Krakow, Poland
autor
  • Institute of Environmental Sciences, Jagiellonian University, Krakow, Poland
autor
  • Institute of Environmental Sciences, Jagiellonian University, Krakow, Poland
Bibliografia
  • Botstein D, Fink GR (2011) Yeast: an experimental organism for 21st century biology. Genetics 189: 695-704. 
  • Bruckner S, Mosch HU (2012) Choosing the right lifestyle: adhesion and development in Saccharomyces cerevisiae. Fems Microbiol Rev 36: 25-58. 
  • Cubillos FA, Louis EJ, Liti G (2009) Generation of a large set of genetically tractable haploid and diploid Saccharomyces strains. Fems Yeast Res 9: 1217-1225. 
  • Diezmann S, Dietrich FS (2009) Saccharomyces cerevisiae: population divergence and resistance to oxidative stress in clinical, domesticated and wild isolates. PLoS One 4: e5317. 
  • Gietz RD, Woods RA (2002) Transformation of yeast by lithium acetate/single-stranded carrier DNA/polyethylene glycol method. Methods Enzymol 350: 87-96. 
  • Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG (1996) Life with 6000 genes. Science 274: 546-567. 
  • Koschwanez JH, Foster KR, Murray AW (2011) Sucrose utilization in budding yeast as a model for the origin of undifferentiated multicellularity. PLoS Biol 9: e1001122. 
  • Liti G, Carter DM, Moses AM, Warringer J, Parts L, James SA, Davey RP, Roberts IN, Burt A, Koufopanou V, Tsai IJ, Bergman CM, Bensasson D, O'Kelly MJ, van Oudenaarden A, Barton DB, Bailes E, Nguyen AN, Jones M, Quail MA, Goodhead I, Sims S, Smith F, Blomberg A, Durbin R, Louis EJ (2009) Population genomics of domestic and wild yeasts. Nature 458: 337-341. 
  • Mewes HW, Albermann K, Bahr M, Frishman D, Gleissner A, Hani J, Heumann K, Kleine K, Maierl A, Oliver SG, Pfeiffer F, Zollner A (1997) Overview of the yeast genome. Nature 387: 7-8. 
  • Mortimer RK, Johnston JR (1986) Genealogy of principal strains of the yeast genetic stock center. Genetics 113: 35-43. 
  • Piccirillo S, Honigberg SM (2010) Sporulation patterning and invasive growth in wild and domesticated yeast colonies. Res Microbiol 161: 390-398. 
  • Schacherer J, Shapiro JA, Ruderfer DM, Kruglyak L (2009) Comprehensive polymorphism survey elucidates population structure of Saccharomyces cerevisiae. Nature 458: 342-345. 
  • Yvert G, Brem RB, Whittle J, Akey JM, Foss E, Smith EN, Mackelprang R, Kruglyak L (2003) Trans-acting regulatory variation in Saccharomyces cerevisiae and the role of transcription factors. Nat Genet 35: 57-64. 
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-23b28ae0-3dfb-471f-bbcb-b6cf7fff913b
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