Comparison of methods for obtaining doubled haploids of carrot

• Autorzy: Kiszczak W. , Kowalska U. , Kapuscinska A. , Burian M. , Gorecka K.
• Języki publikacji: EN

Abstrakty

EN

Doubled haploid lines of carrot can be obtained through androgenesis in anther cultures and in isolated microspore cultures. The two methods were compared using three carrot cultivars (‘Kazan F1’, ‘Feria F1’, and ‘Narbonne F1’) at the androgenesis induction stage, during plant regeneration from embryos, and during acclimatization of androgenetic plants as well as their characterization. It was found that cultivar was the main factor affecting the efficiency at each stage of plant production in both anther and isolated microspore cultures. The efficiency of androgenesis in anther cultures of ‘Feria F1’ was considerably higher in comparison with isolated microspore cultures, and more plants were obtained from the embryos of androgenesis-cultured plants. In ‘Kazan F1’ and ‘Narbonne F1’, more acclimatized androgenetic plants were produced from anther cultures. Ploidy assessment of acclimatized plants of ‘Narbonne F1’ showed that the majority of the plants in the population derived from anther cultures had a doubled chromosome (DH) set. On the other hand, the majority of plants obtained from isolated microspore cultures were haploids. When assessing homozygosity, it was found among plants obtained in anther cultures that the percentage of homozygotes for phosphoglucose isomerase (PGI) and aspartate aminotransferase (AAT) depended on the cultivar. In contrast, the majority of plants derived from isolated microspore cultures were homozygous regardless of cultivar.

• Wydawca: -
• Czasopismo: Acta Societatis Botanicorum Poloniae
• Rocznik: 2017
• Tom: 86
• Numer: 2
• Opis fizyczny: Article 3547 [10p.],fig.,ref.

Twórcy

autor

Kiszczak W.

• Research Institute of Horticulture, Konstytucji 3 Maja 1/3, 96-100 Skierniewice, Poland

autor

Kowalska U.

• Research Institute of Horticulture, Konstytucji 3 Maja 1/3, 96-100 Skierniewice, Poland

autor

Kapuscinska A.

• Research Institute of Horticulture, Konstytucji 3 Maja 1/3, 96-100 Skierniewice, Poland

autor

Burian M.

• Research Institute of Horticulture, Konstytucji 3 Maja 1/3, 96-100 Skierniewice, Poland

autor

Gorecka K.

• Research Institute of Horticulture, Konstytucji 3 Maja 1/3, 96-100 Skierniewice, Poland

Bibliografia

• 1. Maraschin SF, de Priester W, Spaink HP, Wang M. Androgenic switch: an example of plant embryogenesis from the male gametophyte perspective. J Exp Bot. 2005;56:1711– 1726. https://doi.org/10.1093/jxb/eri190
• 2. Germana MA. Anther culture for haploid and doubled haploid production. Plant Cell Tissue Organ Cult. 2011;104(3):283–300. https://doi.org/10.1007/s11240-010-9852-z
• 3. Górecka K, Krzyżanowska D, Kiszczak W, Górecki R. Embryo induction in anther culture of Daucus carota L. Vegetable Crops Research Bulletin. 2005;63:25–32.
• 4. Cegielska-Taras T, Tykarska L, Szała L, Kuraś J, Krzymański J. Direct plant development from microspore-derived embryos of winter oilseed rape Brassica napus L. ssp. oleifera (DC.) Metzger. Euphytica. 2002;124–341. https://doi.org/10.1023/A:1015785717106
• 5. Babbar SB, Agarwal PK, Sahay S, Bhojwani SS. Isolated microspore culture of Brassica: an experimental tool for developmental studies and crop improvement. Indian Journal of Biotechnology. 2004;3:185–202.
• 6. Ferrie AMR. Doubled haploid production in nutraceutical species. Euphytica. 2007;158:347–357. https://doi.org/10.1007/s10681-006-9242-0
• 7. Ferrie AMR, Möllers C. Haploids and doubled haploids in Brassica spp. for genetic and genomic research. Plant Cell Tissue Organ Cult. 2011;104:375–386. https://doi.org/10.1007/s11240-010-9831-4
• 8. Andersen SB, Christiansen I, Farestveit B. Carrot (Daucus carota L.). In vitro production of haploids and field trials. In: Bajaj YPS, editor. Haploids in crop improvement I. Berlin: Springer; 1990. p. 393–402. (Biotechnology in Agriculture and Forestry; vol 12). https://doi.org/10.1007/978-3-642-61499-6_20
• 9. Hu KL, Matsubara S, Murakami K. Haploid plant production by anther culture in carrot (Daucus carrota L.). Journal of the Japanese Society for Horticultural Science. 1993;62(3):561–565. https://doi.org/10.2503/jjshs.62.561
• 10. Tyukavin GB, Shmykova NA, Monakhova MA. Cytological study of embryogenesis in cultured carrot anthers. Russ J Plant Physiol. 1999;46(6):767–773.
• 11. Górecka K, Krzyżanowska D, Górecki R. The influence of several factors on the efficiency of androgenesis in carrot. J Appl Genet. 2005;46(3):265–269.
• 12. Kiszczak W, Górecka K, Krzyżanowska D, Kowalska U. Size of flower buds in carrot (Daucus carota L.) as an indicator of a stage of microsporogenesis and its suitability for induction of androgenesis. Vegetable Crops Research Bulletin. 2005;62:81–90.
• 13. Krzyżanowska D, Górecka K, Kiszczak W, Kowalska U. The effect of genotype and medium on plant regeneration from androgenic embryos. Journal of Fruit and Ornamental Plant Research. 2006;14(1):121–127.
• 14. Kowalska U, Rybaczek D, Krzyżanowska D, Kiszczak W, Górecka K. Cytological assessment of carrot plants obtained in anther culture. Acta Biol Crac Ser Bot. 2008;50(2):7–11.
• 15. Kiszczak W, Krzyżanowska D, Strycharczuk K, Kowalska U, Wolko B, Górecka K. Determination of ploidy and homozygosity of carrot plants obtained from anther cultures. Acta Physiol Plant. 2011;33(2):401–407. https://doi.org/10.1007/s11738-010-0559-x
• 16. Matsubara S, Dohya N, Murakami K, Nishio T, Dore C. Callus formation and regeneration of adventitious embryos from carrot, fennel and mitsuba microspores by anther and isolated microspore cultures. Acta Hortic. 1995;39(2):129–137. https://doi.org/10.17660/ActaHortic.1995.392.15
• 17. Górecka K, Kowalska U, Krzyżanowska D, Kiszczak W. Obtaining carrot (Daucus carota L.) plants in isolated microspore cultures. J Appl Genet. 2010;51(2):141–147. https://doi.org/10.1007/BF03195722
• 18. Li JR, Zhuang FY, Ou CG, Hu H, Zhao ZW, Mao JH. Microspore embryogenesis and production of haploid and doubled haploid plants in carrot (Daucus carota L.). Plant Cell Tissue Organ Cult. 2013;112:275–287. https://doi.org/10.1007/s11240-012-0235-5
• 19. Gamborg OL, Miller RA, Ojima K. Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res. 1968;50:148–151. https://doi.org/10.1016/0014-4827(68)90403-5
• 20. Keller WA, Armstrong KC. Embryogenesis and plant regeneration in Brassica napus anther cultures. Can J Bot. 1977;55:1383–1388. https://doi.org/10.1139/b77-160
• 21. Górecka K, Krzyżanowska D, Kiszczak W, Kowalska U. Plant regeneration from carrot (Daucus carota L.) anther culture derived embryos. Acta Physiol Plant. 2009;31(6):1139– 1145. https://doi.org/10.1007/s11738-009-0332-1
• 22. Galbraith DW. Flow cytometric analysis of the cell cycle. In: Vasil IK, editor. Cell culture and somatic cell genetics of plant. Orlando, FL: Academic Press; 1984. p. 765–777. https://doi.org/10.1016/b978-0-12-715001-7.50090-1
• 23. Gottlieb LD. Enzyme differentiation and phylogeny in Clarkia franciscana, C. rubicunda and C. amoena. Evolution. 1973;27:205–214. https://doi.org/10.1111/j.1558-5646.1973.tb00666.x
• 24. Selander RK, Smith MH, Yang SY, Johnson, WE, Gentry JB. Biochemical polymorphism and systematics in the genus Peromyscus. I. Variation in the old-field mouse (Peromyscus polionotus). Austin, TX: University of Texas; 1971. (Studies in Genetics; vol 6).
• 25. Górecka K, Krzyżanowska D, Kiszczak W, Kowalska U, Górecki R. Obtaining homozygous carrot plants with the aid of androgenesis in anther cultures. Biotechnologia. 2008;2(81):142–152.
• 26. Möllers C, Iqbal MCM. Doubled haploids in breeding winter oilseed rape. In: Touraev A, Forster BP, Mochan Jain S, editors. Advances in haploid production in higher plants. Dordrecht: Springer; 2009. p. 161–169. https://doi.org/10.1007/978-1-4020-8854-4_13
• 27. Górecki R, Górecka K, Krzyżanowska D, Kozik E, Nowak R, Adasik B. Rooting and adaptation of androgenetic carrot plants. Vegetable Crops Research Bulletin. 2001;54:25– 28.
• 28. Tjukavin GB. Osnovy biotehnologii morkovi. Moscow: VNIISSOK; 2007.
• 29. Foisset N, Delourme R, Lugas MO, Renard M. Segregation analysis of isozyme markers on isolated microspore-derived embryos in Brassica napus L. Plant Breed. 1993;110:315– 322. https://doi.org/10.1111/j.1439-0523.1993.tb00595.x

Identyfikatory

DOI

10.5586/asbp.3547