Low-glucose medium induces ORP150 expression and exerts inhibitory effect on apoptosis and senescence of human breast MCF7 cells

• Autorzy: Kretowski R. , Stypulkowska A. , Cechowska-Pasko M.
• Języki publikacji: EN

Abstrakty

EN

 Glucose deprivation is a factor evoking endoplasmic reticulum (ER) stress and induction of expression of an oxygen-regulated protein of 150 kDa (ORP150). We studied the effect of inducible overexpression of ORP150 on senescence and apoptosis of human breast carcinoma cells (MCF7) and human skin fibroblasts. We found an inhibitory effect of ORP150 on apoptosis and senescence of MCF7 cells, but not fibroblasts in ER stress conditions. An increased expression of senescence-associated β-galactosidase and acid β-galactosidase activity (biomarkers of cellular senescence) was observed. We suggest that ORP150 induction in cancer cells can promote tumour progression and may be a major cause of their resistance to chemotherapeutics.

Słowa kluczowe

EN

endoplasmic reticulum; low-glucose medium; protein expression; molecular mass; inhibitory effect; apoptosis; senescence; human breast; MCF-7 cell line; beta-galactosidase; cancer cell; chemotherapeutic drug

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2013
• Tom: 60
• Numer: 2
• Opis fizyczny: p.167-173,fig.,ref.

Twórcy

autor

Kretowski R.

• Department of Pharmaceutical Biochemistry, Medical University of Bialystok, Bialystok, Poland

autor

Stypulkowska A.

• Department of Pharmaceutical Biochemistry, Medical University of Bialystok, Bialystok, Poland

autor

Cechowska-Pasko M.

• Department of Pharmaceutical Biochemistry, Medical University of Bialystok, Bialystok, Poland

Bibliografia

• Chang BD, Broude EV, Dokmanovic M, Zhu H, Ruth A, Xuan Y, Kandel ES, Lausch E, Christov K, Roninson IB (1999a) A senescence-like phenotype distinguishes tumor cells that undergo terminal proliferation arrest after exposure to anticancer agents. Cancer Res 59: 3761-3767. 
• Chang BD, Xuan Y, Broude EV, Zhu H, Schott B, Fang J, Roninson IB (1999b) Role of p53 and p21waf1/cip1 in senescence-like terminal proliferation arrest induced in human tumor cells by chemotherapeutic drugs. Oncogene 18: 4808-4818. 
• Chatterjee S, Velicer LF, Sweeley CC (1975) Glycosphingolipid glycosyl hydrolases and glycosidases of synchronized human KB cells. J Biol Chem 250: 4972-4979. 
• Chen Z, Trotman LC, Shaffer D, Lin HK, Dotan ZA, Niki M, Koutcher JA, Scher HI, Ludwig T, Gerald W, Cordon-Cardo C, Pandolfi PP (2005) Crucial role of p53-dependent cellular senescence in suppression of Pten-deficient tumorigenesis. Nature 436: 725-730. 
• Collado M, Blasco MA, Serrano M (2007) Cellular senescence in cancer and aging. Cell 130: 223-233. 
• Dimri GP (2005) What has senescence got to do with cancer? Cancer Cell 7: 505-512. 
• Flores-Diaz M, Higuita JC, Florin I, Okada T, Pollesello P, Bergman T, Thelestam M, Mori K, Alape-Giron A (2004) A cellular UDP-glucose deficiency causes overexpression of glucose/oxygen-regulated proteins independent of the endoplasmic reticulum stress elements. J Biol Chem 279: 21724-21731. 
• Harding HP, Calfon M, Urano F, Novoa I, Ron D (2002) Transcriptional and translational control in the Mammalian unfolded protein response. Annu Rev Cell Dev Biol 18: 575-599. 
• Jolly C, Morimoto RI (2000) Role of the heat shock response and molecular chaperones in oncogenesis and cell death. J Natl Cancer Inst 92: 1564-1572. 
• Kaufman RJ, Scheuner D, Schroder M, Shen X, Lee K, Liu CY, Arnold SM (2002) The unfolded protein response in nutrient sensing and differentiation. Nat Rev Mol Cell Biol 3: 411-421. 
• Kurz DJ, Decary S, Hong Y, Erusalimsky JD (2000) Senescence-associated (beta)-galactosidase reflects an increase in lysosomal mass during replicative ageing of human endothelial cells. J Cell Sci 113: 3613-3622. 
• Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685. 
• Lowe SW, Cepero E, Evan G (2004) Intrinsic tumour suppression. Nature 432: 307-315. 
• Lundberg AS, Hahn WC, Gupta P, Weinberg RA (2000) Genes involved in senescence and immortalization. Curr Opin Cell Biol 12: 705-709. 
• Ma Y, Hendershot LM (2001) The unfolding tale of the unfolded protein response. Cell 107: 827-830. 
• Mason DX, Jackson TJ, Lin AW (2004) Molecular signature of oncogenic ras-induced senescence. Oncogene 23: 9238-9246. 
• Matsushita K, Matsuyama T, Nishimura H, Takaoka T, Kuwabara K, Tsukamoto Y, Sugita M, Ogawa S (1998) Marked, sustained expression of a novel 150-kDa oxygen-regulated stress protein, in severely ischemic mouse neurons. Mol Brain Res 60: 98-106. 
• Mosser DD, Morimoto RI (2004) Molecular chaperones and the stress of oncogenesis. Oncogene 23: 2907-2918. 
• Narita M, Lowe SW (2004) Executing cell senescence. Cell Cycle 3: 244-246. 
• Nishigaki T, Okada S (1998) [beta-Galactosidosis (acid beta-galactosidase deficiency: GM1 gangliosidosis, Morquio B disease)]. Ryoikibetsu Shokogun Shirizu 403-406.
• Noppe G, Dekker P, de Koning-Treurniet C, Blom J, van Heemst D, Dirks RW, Tanke HJ, Westendorp RG, Maier AB (2009) Rapid flow cytometric method for measuring senescence associated beta-galactosidase activity in human fibroblasts. Cytometry A 75: 910-916. 
• Palaniyappan A (2009) Cyclophosphamide induces premature senescence in normal human fibroblasts by activating MAP kinases. Biogerontology 10: 677-682. 
• Parkinson EK, Munro J, Steeghs K (2005) Replicative senescence as a barrier to human cancer. Biochem Soc Trans 28: 226-233. 
• Roninson IB (2003) Tumor cell senescence in cancer treatment. Cancer Res 63: 2705-2715. 
• Saretzki G (2010) Cellular senescence in the development and treatment of cancer. Curr Pharm Des 16: 79-100. 
• Severino J, Allen RG, Balin S, Balin A, Cristofalo VJ (2000) Is beta-galactosidase staining a marker of senescence in vitro and in vivo? Exp Cell Res 257: 162-171. 
• Sherman M, Multhoff G (2007) Heat shock proteins in cancer. Ann N Y Acad Sci 1113: 192-201. 
• Smith PK, Krohn RI, Hermanson GT, Mallia AK, Gartner FH, Provenzano MD, Fujimoto EK, Goeke NM, Olson BJ, Klenk DC (1985) Measurement of protein using bicinchoninic acid. Anal Biochem 150: 76-85. 
• Soti C, Sreedhar AS, Csermely P (2003) Apoptosis, necrosis and cellular senescence: chaperone occupancy as a potential switch. Aging Cell 2: 39-45. 
• Tsukamoto Y, Kuwabara K, Hirota S, Ikeda J, Stern D, Yanagi H, Matsumoto M, Ogawa S, Kitamura Y (1996) 150-kD oxygen-regulated protein is expressed in human atherosclerotic plaques and allows mononuclear phagocytes to withstand cellular stress on exposure to hypoxia and modified low density lipoprotein. J Clin Invest 98: 1930-1941. 
• Tsukamoto Y, Kuwabara K, Hirota S, Kawano K, Yoshikawa K, Ozawa K, Kobayashi T, Yanagi H, Stern DM, Tohyama M, Kitamura Y, Ogawa S (1998) Expression of the 150-kd oxygen-regulated protein in human breast cancer. Lab Invest 78: 699-706. 
• Yi TN, Zhao HY, Zhang JS, Shan HY, Meng X, Zhang J (2009) Effect of aspirin on high-glucose-induced senescence of endothelial cells. Chin Med J 122: 3055-3061. 
• Yoshida H (2007) ER stress and diseases. Febs J 274: 630-658.