Inhibition of in vitro Histophilus somni biofilm production by recombinat Hsp60 antibodies
Treść / Zawartość
Histophilus somni is an opportunistic pathogen causing respiratory, genitourinary and generalized infections in cattle. An important virulence factor is its ability to produce a biofilm. The aim of this work was to confirm that H. somni Hsp60 (Gro-EL) is a constituent of the biofilm produced by this bacterium in vitro and to check whether or not the presence of a specific antibody within the culture medium can inhibit biofilm production. Biofilm production by H. somni cultured in vitro was confirmed by crystalline violet staining. The presence of Hsp60 in the biofilm was confirmed by using specific antibodies produced in a mouse and goat hyperimmunized with H. somni recombinant Hsp60 (rHsp60). Large complexes of biofilm stained with Hsp60 antibodies were microscopically detected. This indicates that the Hsp60 protein is a common constituent of the biofilm produced by H. somni in vitro. In a second experiment, mouse serum containing anti- H. somni rHsp60 antibodies was added to an H. somni culture. It was found that the presence of anti-rHsp60 antibodies in the culture medium inhibited biofilm production in vitro. Only small biofilm particles were seen in the presence of the specific antibody, whereas in control cultures (without specific antiserum) large biofilm complexes were produced. The results indicate that antibodies specific to Hsp60 may be useful for preventing H. somni biofilm formation in vitro. If this also occurs in vivo, it may be helpful for eradicating H. somni infection in cattle through the elimination of carriers. Further in vivo studies are needed to confirm this idea.
- Bolhassani A, Rafati S (2008) Heat-shock proteins as powerful weapons in vaccine development. Expert Rev Vaccines 7: 1185-1199.
- Corbeil LB (2007) Histophilus somni host – parasite relationships. Anim Health Res Rev 8: 151-160.
- Davey ME, O’toole GA (2000) Microbial Biofilms: from Ecology to Molecular Genetics. Microbiol. Mol Biol Rev 64: 847-867.
- Kuczyńska-Wiśnik D., Matuszewska E., Laskowska E (2010) Escherichia coli heat-shock proteins IbpA and IbpB affect biofilm formation by influencing the level of extracellular indole. Microbiology 156: 148-157.
- Moxon ER, Sweetman WA, Deadman ME, Ferguson DJ, Hood DW (2008) Haemophilus influenzae biofilms: hypothesis or fact? Trends Microbiol 16: 95-100.
- Sandal I, Hong W, Swords WE, Inzana TJ (2007) Characterization and Comparison of Biofilm Development by Pathogenic and Commensal Isolates of Histophilus somni. J Bacteriol 189: 8179-8185.
- Sandal I, Shao JQ, Annadata S, Apicella MA, Boye M, Jensen TK, Saunders GK, Inzana TJ (2009) Histophilus somni biofilm formation in cardiopulmonary tissue of the bovine host following respiratory challenge. Microbes Infect 11: 254-263.
- Smith DF, Whitesell L, Katsanis E (1998) Molecular chaperones: biology and prospects for pharmacological intervention. Pharmacol Rev 50: 493-514.
- Stefaniak T, Galli J (2008) Znaczenie zakażenia Histophilus somni w etiopatogenezie syndromu oddechowego bydła i jego zwalczanie. In: Bednarek D (ed) Najważniejsze czynniki etiologiczne, patogeneza i najnowsze trendy w profilaktyce i terapii syndromu oddechowego bydła (BRD) Państwowy Instytut Weterynaryjny-Państwowy Instytut Badawczy, Puławy pp 59-76.
- Tsan MF, Gao B (2004) Heat Shock Protein and Innate Immunity. Cell Mol Immunol 1: 274-279.
- Tsan MF, Gao B (2009) Heat shock proteins and immune system. J Leukoc Biol 85: 905-910.
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