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2013 | 35 | 08 |
Tytuł artykułu

Transcriptome analysis reveals differentially expressed genes associated with propamocarb response in cucumber (Cucumis sativus L.) fruit

Autorzy
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
High-throughput tag-sequencing (Tag-seq) from Illumina analysis, which is based on the Solexa Genome Analyzer platform, was applied to analyze the gene expression profiling of propamocarb (PM) treatment and control in cucumber fruit. Approximately 3.6 million complete clean sequence tags at PM treatment or control library were obtained with approximately 0.1 million distinct clean tag sequences. Approximately 41.79–43.15 % of the distinct clean tags were mapped unambiguously to the unigene database, and 32.54–33.46 % of the distinct clean tags were mapped to the cucumber genome database. The profiling analysis of the differentially expressed genes revealed the up-regulation of 546 genes and the downregulation of 185 genes with PM response. Furthermore, the differentially expressed genes mainly linked to pesticide detoxication, response to stress/stimulus, transporter/ signaling, and some important transcription factors. Finally, quantitative real-time polymerase chain reaction (qRT-PCR) and reverse transcription polymerase chain reaction (RT-PCR) using 16 genes independently verified the tag-mapped results. The present study reveals the comprehensive mechanisms of PM response in cucumber fruit.
Słowa kluczowe
EN
Wydawca
-
Rocznik
Tom
35
Numer
08
Opis fizyczny
p.2393-2406,fig.,ref.
Twórcy
autor
  • College of Horticulture, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Northeast Agricultural University, Ministry of Agriculture, 59 Mucai Road, Harbin 150030, People’s Republic of China
autor
  • College of Horticulture, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Northeast Agricultural University, Ministry of Agriculture, 59 Mucai Road, Harbin 150030, People’s Republic of China
autor
  • College of Horticulture, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Northeast Agricultural University, Ministry of Agriculture, 59 Mucai Road, Harbin 150030, People’s Republic of China
autor
  • College of Horticulture, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Northeast Agricultural University, Ministry of Agriculture, 59 Mucai Road, Harbin 150030, People’s Republic of China
autor
  • College of Horticulture, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Northeast Agricultural University, Ministry of Agriculture, 59 Mucai Road, Harbin 150030, People’s Republic of China
autor
  • College of Horticulture, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Northeast Agricultural University, Ministry of Agriculture, 59 Mucai Road, Harbin 150030, People’s Republic of China
autor
  • College of Horticulture, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Northeast Agricultural University, Ministry of Agriculture, 59 Mucai Road, Harbin 150030, People’s Republic of China
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Bibliografia
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Identyfikator YADDA
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