Genetic variability and host specialization in Alternaria alternata colonizing Solanaceous crops in Sudan

• Autorzy: Abbo A.S. , Idris M.O. , Elballa M.A. , Hammad A.M. , El Sidding M.A.R. , Karlovsky P.
• Języki publikacji: EN

Abstrakty

EN

Early blight disease caused by Alternaria sp. is one of the most devastating diseases of Solanaceous crops widely distributed in Sudan. The aim of this study was to determine the genetic variation among different Alternaria isolates recovered from different Solanaceae crops showing typical symptoms of early blight disease. Infected leaves of tomato, potato, eggplant and pepper were collected from different geographical zones in Sudan. The recovered fungal isolates were identified to the genus level based on cultural and morphological characteristics. Five representative isolates were sent to the CABI Bioscience, U.K. for confirmation. The genetic relationship among the isolates was determined using the amplified fragments length polymorphism (AFLP) technique and the generated data were used to create similarity matrices using the PAST 3.01 software package. Dendrograms were constructed based on Jaccard’s similarity coefficients. A total of 70 fungal isolates was recovered from the tested plants and all of them showed morphological characteristics typical of Alternaria spp. The conidia appeared in multiple-branched chains with spore sizes in the range of 2.38−13.09 μm × 12.30−43.63 μm. Therefore, the isolates were identified as Alternaria alternata (Fr.) Keissl. The identification was then confirmed by CABI.AFLPbased dendrogram which revealed five clusters with a significant cophenetic correlation coefficient (r = 0.834) between the dendrogram and the original similarity matrix irrespective of their geographical origins. Eighteen (75%) of the Alternaria isolated from tomato leaves were clustered together in cluster I and five isolates formed two separate clusters, viz. cluster IV (T-Kh5 and T-H1) and cluster V (T-H4 and T-Med2). The remaining isolate, T-Am5, grouped with one of the potato isolates in cluster III. The other isolates which were recovered from potato, pepper and eggplants were all separated from the tomato isolates in the largest cluster.

Słowa kluczowe

EN

plant disease; early blight; genetic variability; host; Alternaria alternata; diversity; Solanaceae; crop; amplified fragment length polymorphism; Sudan

• Wydawca: -
• Czasopismo: Journal of Plant Protection Research
• Rocznik: 2018
• Tom: 58
• Numer: 3
• Opis fizyczny: p.246-256,fig.,ref.

Twórcy

autor

Abbo A.S.

• Department of Crop Protection, University of Khartoum, Khartoum, Sudan

autor

Idris M.O.

• Department of Crop Protection, University of Khartoum, Khartoum, Sudan

autor

Elballa M.A.

• Department of Horticulture, University of Khartoum, Khartoum, Sudan

autor

Hammad A.M.

• Department of Crop Protection, University of Khartoum, Khartoum, Sudan

autor

El Sidding M.A.R.

• Department of Botany, University of Khartoum, P.O.Box 321, Khartoum, Sudan

autor

Karlovsky P.

• Molecular Phytopathology and Mycotoxin Research, Georg-August University, 37077 Gottingen, Germany

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Identyfikatory

DOI

10.24425/122940