Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2011 | 53 | 2 |
Tytuł artykułu

Micropropagation of Codonopsis pilosula (Franch.) Nannf by axillary shoot multiplication

Treść / Zawartość
Warianty tytułu
Języki publikacji
Roots of Codonopsis pilosula (Franch.) Nannf. are among the most popular Chinese herbal medicines, exhibiting various beneficial activities which support immunity and stress resistance. The plant shows high intraspecific genetic variation. There is a need for effective vegetative propagation methods yielding high and sustainable quality. Here we report a micropropagation method using axillary shoot proliferation. Nodal segments from aseptically germinated plants were inoculated on modified MS media enriched with different concentrations of cytokinins: benzyladenine, kinetin (1, 4, 10 or 20 μM) or thidiazuron (1, 4 or 8 μM), with or without the auxin NAA (1 μM). Axillary bud break was initiated most efficiently on media with 1 or 4 μM BA and 1μM NAA. Shoot number increased markedly in subsequent cycles of harvesting and transfer to fresh 1 μM BA and NAA medium, leading to the maximum 69 shoots (mean 38.16±4.35) from a single nodal explant in the fourth harvest. The shoots were successfully (>98% efficiency) rooted in MS medium containing high sucrose (60 g/L) and 5 μM IAA, and acclimatized to soil cultivation with a survival rate of 90%. These results can be used to establish a simple and commercially viable protocol for mass propagation of C. pilosula for plantations or breeding.
Słowa kluczowe
Opis fizyczny
  • Department of Pharmaceutical Biology and Botany, Medical University of Wroclaw, Al. Jana Kochanowskiego 10, Wroclaw, Poland
  • CHAN JYW, LEUNG PC, CHE CT, and FUNG KP. 2008. Protective effects of an herbal formulation of Radix Astragali, Radix Codonopsis and Cortex Lycii on Streptozotocin-induced apoptosis in pancreatic β-cells: An implication for its treatment of diabetes mellitus. Phytotherapy Research 22: 190–196.
  • CHATURVEDI HC, JAIN M, and KIDWAI NR. 2007. Cloning of medicinal plants through tissue culture – A review. Indian Journal Experimental Biology 45: 937–948.
  • CRAKER LE, and GIBLETTE J. 2002. Chinese medicinal herbs: Opportunities for domestic production. In: Janick J, Whipkey A [eds.], Trends in New Crops and New Uses, 491–496. ASHS Press, Alexandria, VA.
  • CHEN CC, CHEN SJ, SAGARE AP, and TSAY HS. 2001. Adventitious shoot regeneration from stem internode explants of Adenophora triphylla (Thunb.) A. DC. (Campanulaceae) – an important medicinal herb. Botanical Bulletin of Academia Sinica 42: 1–7.
  • CHEN JK, CHEN TT. 2004. Chinese Medical Herbology and Pharmacology. Art of Medicine Press, City of Industry, CA, USA.
  • FLORA OF CHINA. 2011. Vol. 19, 513–526. Internet:, access on Apr.1st 2011.
  • GHIMIRE BK, SEONG ES, LIM JD, HEO K, KIM MJ, CHUNG IM, JUVIK JA, and YU CY. 2008. Agrobacterium-mediated transformation of Codonopsis lanceolata using the γ-TMT gene. Plant Cell Tissue and Organ Culture 95: 265–274.
  • GUO WL, GONG L, DING ZF, LI YD, LI FX, ZHAO SP, and LIU B. 2006. Genomic instability in phenotypically normal regenerants of medicinal plant Codonopsis lanceolata Benth. et Hook., as revealed by ISSR and RAPD markers. Plant Cell Reports 25: 896–906.
  • GUO HB, LU BR, WU QH, CHEN JK, and ZHOU TS. 2007. Abundant genetic diversity in cultivated Codonopsis pilosula populations revealed by RAPD polymorphisms. Genetic Resources and Crop Evolution 54, 917–924.
  • HEMPEN CH, and FISCHER T. 2009. A Materia Medica for Chinese Medicine. Churchill Livingstone Elsevier, Edinburgh, UK. HONG DY. 2010. Taxonomic notes on Chinese Campanulaceae. Novon 20: 420–425.
  • HU SY. 2005. Food Plants of China. Chinese University Press, Hong-Kong.
  • LI JS, JIA JF, and QI FJ. 1993. Plant regeneration from callus protoplasts of Codonopsis pilosula. Acta Botanica Sinica 35: 864–867.
  • LI ZH, WEN HY, CHEN J, WU GL, and WANG YJ. 2009a. Development of 10 polymorphic microsatellite loci primers for Codonopsis pilosula Nannf. (Campanulaceae). Conservation Genetics 10: 747–749.
  • LI CY, XU HX, HAN QB, and WU TS. 2009b. Quality assessment of Radix Codonopsis by quantitative nuclear magnetic resonance. Journal of Chromatography A 1216: 2124–2129.
  • LIU GZ, CAI DG, and SHAO S. 1988. Studies on the chemical constituents and pharmacological actions of dangshen, Codonopsis pilosula (Franch.) Nannf. Journal of Traditional Chinese Medicine 8: 41–47.
  • MIN RS, YANG SG, LIU JR, SONG PS, and SOH WY. 1992. High frequency somatic embryogenesis and plant regeneration in tissue cultures of Codonopsis lanceolata. Plant Cell Reports 10: 621 623.
  • MURASHIGE T, and SKOOG F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15: 473–497.
  • NALAWADE SM, and TSAI HS. 2004. In vitro propagation of some important Chinese medicinal plants and their sustainable usage. In Vitro Cellular and Developmental Biology – Plant 40: 143–154.
  • NIU D, SHAO Q, and ZHANG J. 1991. Studies of in vitro culture and plantlet regeneration of Codonopsis pilosula Nannfl. Acta Genetica Sinica 18: 168–174.
  • RAKHIMOV DA, SALIMOVA ES, and ZAKIROVA RP. 2003. Polysaccharides of plant tissue culture. III. Polysaccharides of Codonopsis clematidea callus culture. Chemistry of Natural Compounds 39: 46–47.
  • SAHA S, DEY P, and GHOSH T. 2010. Micropropagation of Ocimum kilimandscharicum Guerke (Labiatae). Acta Biologica Cracoviensia Series Botanica 52: 50–58.
  • SARASAN V, CRIPPS R, RAMSAY MM, ATHERTON C, MCMICHEN M, PRENDERGAST G, and ROWNTREE JK. 2006. Conservation in vitro of threatened plants-progress in the past decade. In Vitro Cellular and Developmental Biology – Plant 42: 2006–2014.
  • WAKANA D, KAWAHARA N, and GODA Y. 2011. Three new triterpenyl esters, codonopilates A-C, isolated from Codonopsis pilosula. Journal of Natural Medicines 65: 18–23.
  • YANG J, SU Q, LIU E, and GAO J. 2010. Assay of lobetyolin and atractylenolide III in radix Codonopsis from different habitats. Shanxi Yike Daxue Xuebao 41: 698–702.
  • ZHANG YB, NGAN FN, WANG ZT, NG TB, BUT PP, SHAW PC, and WANG J. 1999. Random primed polymerase chain reaction differentiates Codonopsis pilosula from different localities. Planta Medica 65: 157–160.
  • ZYCH M, FURMANOWA M, KRAJEWSKA-PATAN A, ŁOWICKA A, DREGER M, and MENDLEWSKA S. 2005. Micropropagation of Rhodiola kirilowii plants using encapsulated axillary buds and callus. Acta Biologica Cracoviensia Series Botanica 47: 83–87.
Rekord w opracowaniu
Typ dokumentu
Identyfikator YADDA
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.