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2014 | 44 | 1 |
Tytuł artykułu

Mitochondrial DNA variation in the endangered fish Dawkinsia tambraparniei (Actinopterygii: Cypriniformes: Cyprinidae) from southern Western Ghats, India

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Background. Dawkinsia tambraparniei (Silas, 1954) is confined to an area not exceeding 100 km2 within a single watershed—the Tamiraparani River, southern Western Ghats, India. Its populations have recently declined, earning the fish the endangered status. For effective conservation efforts it is important to determine the levels of genetic variation between different populations. Therefore we attempted to quantify the cytochrome b (Cyt b) and cytochrome c oxidase subunit I (COI) gene sequence similarity between different populations of D. tambraparniei, as well as to study the phylogenetic relation with the closely related species D. arulius and D. filamentosa. Materials and methods. Samples were collected from 9 locations and 10 individuals from each were preserved for DNA analysis. The partial sequence of Cyt b and COI genes were amplified and sequenced. The sequences were aligned by the visual method using BioEdit version 7.1.3.0 and edited using Sequencer 4.7. The Bayesian consensus tree was constructed using the Monte Carlo Markov Chain (MCMC) method by BEAST v1.7.5. Results. The partial sequences of Cyt b and COI from 90 specimens of Dawkinsia tambraparniei representing nine populations were examined. The Cyt b gene sequences showed that all populations belong to a single haplotype and no nucleotide variation was observed between populations. In the case of COI gene sequences, two haplotypes were revealed: an additional haplotype with a clear transition of C to T at the nucleotide 58 position was detected within the Thalayanai Stream population. The phylogenetic analysis of COI gene demonstrated that the pair wise genetic distance between D. tambraparniei and D. arulius was low (0.004 ± SE 0.003) when compared with the distance between D. tambraparniei and D. filamentosa (0.061 ± SE 0.012). Conclusion. The mitochondrial DNA analysis revealed very low diversity among investigated populations of D. tambraparniei for studied genes, with the only local population from the Thalayanai Stream demonstrating certain divergence. The phylogenetic analysis confirms the concept on the validity of D. tambraparniei as a separate species. This phylogenetic study also demonstrated that D. tambraparniei is closer to D. arulius, followed by D. filamentosa.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
44
Numer
1
Opis fizyczny
p.3-8,fig.,ref.
Twórcy
autor
  • Wildlife Institute of India, 18 Chandrabani, Dehradun, 248001, Uttarkhand, India
  • Department of Habitat Ecology, Wildlife Institute of India, 18, Chandrabani, Dehradun 248 001, Uttarakhand, India
autor
  • Wildlife Institute of India, 18 Chandrabani, Dehradun, 248001, Uttarkhand, India
autor
  • Wildlife Institute of India, 18 Chandrabani, Dehradun, 248001, Uttarkhand, India
Bibliografia
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  • Arunachalam M., Murugan M. 2007. Cytogenetics and cytotaxonomic consideration of two endangered ornamental fishes Puntius arulius and P. tambraparniei (Cypriniformes: Cyprinidae) from Western Ghats, India. Zoo’s Print Journal 22 (7): 2739–2741.
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  • Dahanukar N. 2011. Puntius tambraparniei. In: Anonymous (eds.) IUCN red list of threatened species. Version 2011.2. http://iucnredlist.org. Accessed on 30 January 2013.
  • Drummond A.J., Rambaut A. 2007. BEAST: Bayesian evolutionary analysis by sampling trees. BMC Evolution Biology 7: 214. DOI: 10.1186/1471-2148-7-214
  • Fang F., Norén M., Liao T.Y., Källersjö M., Kullander S.O. 2009. Molecular phylogenetic interrelationships of the south Asian cyprinid genera Danio, Devario and Microrasbora (Teleostei, Cyprinidae, Danioninae). Zoologica Scripta 38 (3): 237–256. DOI: 10.1111/j.1463-6409.2008.00373.x
  • Finne K.L. 2001. Phylogeographic structure of the Atlantic pupfish, Cyprinodon variegates along the eastern coast of North America. PhD Dissertation, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA.http://scholar.lib.vt.edu/theses/available/etd-04202001-203337
  • Hall T.A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 1999 (41): 95–98.
  • Jerdon T.C. 1849. On the fresh-water fishes of southern India. Madras Journal of Literature and Science 15 (2): 302–346.
  • Pallavi, GoswamiM., Nautiyal P.,Malakar A.K., Nagpure N.S.2012. Genetic divergence and molecular phylogenetics of Puntius spp. based on the mitochondrial cytochrome b gene.Mitochondrial DNA 23 (6): 477–483. DOI: 10.3109/19401736.2012.710209
  • Pethiyagoda R., Kottelat M., 2005. A review of the barbs of the Puntius filamentosus group (Teleostei: Cyprinidae) of southern India and Sri Lanka. Raffles Bulletin of Zoology 2005 (Suppl. 12): 127–144.
  • Sah S., Barat A., Pande V., Sati J., Goel C. 2011. Population structure of Indian hill trout (Barilius bendelisis) inferred from variation in mitochondrial DNA sequences. Advances in Biological Research 5 (2): 93–98.
  • Saitoh K., Sado T.,Mayden R.L., Hanzawa N., Nakamura K., Nishida M., Miya M. 2006. Mitogenomic evolution and interrelationships of the Cypriniformes (Actinopterygii:Ostariophysi): The first evidence toward resolution of higher-level relationships of the world’s largest freshwater fish clade based on 59 whole mitogenome sequences. Journal of Molecular Evolution 63 (6): 826–841. DOI: 10.1007/s00239-005-0293-y
  • Sambrook J., Fritsch E.F., Maniatis T. 1989. Molecular Cloning: A laboratory manual. 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA.
  • Silas E.G. 1954. New fishes from the Western Ghats, with notes on Puntius arulius (Jerdon). Records of Indian Museum 51 (1): 27–38.
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  • Tamura K., Peterson D., Peterson N., Stecher G., Nei M.,Kumar S. 2011. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution 28 (10): 2731–2739. DOI: 10.1093/molbev/msr121
  • Tinti F., Di Nunno C., Guarniero I., TalentiM., Tommasini S.,Fabbri E., Piccinetti C. 2002. Mitochondrial DNA sequence variation suggests the lack of genetic heterogeneity in the Adriatic and Ionian stocks of Sardina pilchardus. Marine Biotechnology 4 (2): 163–172. DOI: 10.1007/s10126-002-0003-3
  • Ward R.D., Zemlak T.S., Innes B.H., Last P.R., Hebert P.D.N. 2005. DNA barcoding Australia’s fish species.Philosophical Transactions of the Royal Society, Part B: Biological Sciences 360 (1462): 1847–1857. DOI: 10.1098/rstb.2005.1716
Typ dokumentu
Bibliografia
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