Comparison of Allium aflatunense B. Fedtsch. ‘Purple Sensation’ and Allium karataviense Regel. ‘Ivory Queen’ regenerative capabilities in tissue culture

• Autorzy: Kozak D. , Stelmaszczuk M.

Warianty tytułu

PL

Porównanie zdolnosci regeneracyjnych Allium aflatunense B. Fedtsch. 'Purple Sensation' i Allium karataviense Regel. 'Ivory Queen' w kulturze in vitro

• Języki publikacji: EN

Abstrakty

EN

Allium aflatunense and Allium karataviense are valuable bulbous plants grown in a garden. Moreover, Allium aflatunense is one of the most decorative species for cut flowers industry. These plants characterize with a low multiplication rate. Immature inflorescence stem sections and leaf bases isolated from ‘Allium aflatunense ‘Purple Sensation’ and Allium karataviense ‘Ivory Queen’ bulbs in October were used as source of explants for shoot regeneration. The use of a two – step sterilization procedure (2% Cl- – 30 min and 1% Cl- –15 min) gave much better results than a one – step (2% Cl- – 30 min). Inflorescence explants in inverted orientation were cultured on a Murashige and Skoog [1962] medium containing BA in concentration of 2 mg·dm⁻³ and NAA in concentration of 1 mg·dm⁻³. Leaf bases placed in normal and inverted orientation were incubated on MS medium supplemented with: BA 2 mg·dm⁻³ plus NAA 1 mg·dm⁻³, BA 5 mg·dm⁻³ plus NAA 1 mg·dm⁻³ and TDZ 1 mg·dm⁻³ plus NAA 1 mg·dm⁻³. In subsequent experiments clumps consisting of 2 shoots were cultured on MS medium containing BA 0.5, 1, 2 mg·dm⁻³ with NAA 0.1 mg·dm⁻³ and BA 2 mg·dm⁻³ with IAA 0.1 mg·dm⁻³ for 6 weeks. A multiplication rate during four subcultures on medium with addition of BA 0.5 mg·dm⁻³ and NAA 0.1 mg·dm⁻³ was also examined. Explants from young inflorescences and from immature leaf bases were found effective to form shoots. The orientation of leaf explants did not have a significant effect on the number of regenerating shoots. 5.7 to 7.5 shoots were achieved for Allium aflatunense ‘Purple Sensation’ on studied media during 6 weeks of multiplication and 3.5 to 5.1 shoots for Allium karataviense ‘Ivory Queen’. Multiplication rate of 31.3 shoots for Allium aflatunense ‘Purple Sensation’ and 26.6 shoots for Allium karataviense ‘Ivory Queen’ was possible to obtain on medium supplemented with BA 0.5 mg·dm⁻³ and NAA 0.1 mg·dm⁻³ after 32 weeks of culture.

PL

Allium aflatunense i Allium karataviense to cenne rośliny cebulowe do uprawy w ogrodach. Ponadto Allium aflatunense jest jednym z najbardziej dekoracyjnych gatunków uprawianym na kwiat cięty. Rośliny te charakteryzują się niskim współczynnikiem rozmnażania. Źródłem materiału do zapoczątkowania kultur były fragmenty młodych pędów kwiatostanowych i nasady liści izolowane w paĨdzierniku z Allium aflatunense ‘Purple Sensation’ i Allium karataviense ‘Ivory Queen’. Zastosowanie sterylizacji dwukrotnej (2% Cl- – 30 min i 1% Cl- –15 min) dało lepsze rezultaty niż sterylizacja pojedyncza. Eksplantaty z pędów kwiatostanowych wykładano w pozycji odwrotnej do naturalnej i kultywowano na pożywce Murashige i Skooga [1962] zawierającej BA 2 mg·dm⁻³ i NAA 1 mg·dm⁻³. Podstawy liści układano w pozycji naturalnej i odwróconej i inkubowano na pożywce MS z dodatkiem: BA 2 mg·dm⁻³ + NAA 1 mg·dm⁻³, BA 5 mg·dm⁻³ + NAA 1 mg·dm⁻³ i TDZ 1 mg·dm⁻³ + NAA 1 mg·dm⁻³. W kolejnym doświadczeniu zespoły pędów zawierające 2 pędy namnażano na pożywkach MS uzupełnionych: BA 0,5; 1; 2 mg·dm⁻³ oraz NAA 0,1 mg·dm⁻³ i BA 2 mg·dm⁻³ oraz IAA 0,1 mg·dm⁻³ podczas 6 tygodni. Oceniono również współczynnik namnażania pędów podczas 4 pasaży na pożywce z dodatkiem BA 0,5 mg·dm⁻³ i NAA 0,1 mg·dm⁻³. Fragmenty młodych pędów kwiatostanowych i podstawa liści wykazywały duże zdolności regeneracyjne pędów. Sposób ułożenia eksplantatów nie wywierał istotnego wpływu na liczbę regenerujących pędów. Na badanych pożywkach uzyskano od 5,7 do 7,5 pędów Allium aflatunense ‘Purple Sensation’ i od 3,5 do 5,1 pędów Allium karataviense ‘Ivory Queen’ podczas 6 tygodni namnażania. Podczas 32 tygodni namnażania pędów na pożywce zawierającej BA 0,5 mg·dm⁻³ i NAA 0,1 mg·dm⁻³ uzyskano 31,3 pędów Allium aflatunense ‘Purple Sensation’ i 26,6 pędów of Allium karataviense ‘Ivory Queen’.

Słowa kluczowe

EN

comparison; Allium aflatunense; ornamental plant; Allium karataviense; regenerative capability; tissue culture; inflorescence stem; leaf base; polarity explant; shoot multiplication

• Wydawca: -
• Czasopismo: Acta Scientiarum Polonorum. Hortorum Cultus
• Rocznik: 2013
• Tom: 12
• Numer: 6
• Opis fizyczny: p.197-213,fig.,ref.

Twórcy

autor

Kozak D.

• Institute of Ornamental Plants and Landscape Architecture, University of Life Sciences in Lublin, 58 Leszczynskiego Str., 20-068 Lublin, Poland

autor

Stelmaszczuk M.

• University of Life Sciences in Lublin, Lublin, Poland

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