EN
The callus tissue can be used to evaluate the potential ability of microorganisms to cause disease. The blue-stain fungi, Grosmannia piceiperda and Ophiostoma tetropii are important associates of Tetropium spp. in Poland. The opinions about their virulence are controversial. Here, we examined the growth mycelium of the G. piceiperda and O. tetropii in presence of the non-embryogenic cultures of Norway spruce, and accumulation of soluble and unsoluble proteins in this callus. The growth mycelium of one isolate of G. piceiperda was significantly stimulated whilst another isolate of the fungus and both isolates of O. tetropii were unaffected by the presence of the callus. The significant higher (P<0.05) amount of soluble protein, was noted in the callus with both isolates of G. piceiperda. In contrast to G. piceiperda, the callus with O. tetropii had a similar concentration of soluble protein as the control. The importance of these results with respect to the pathogenic abilities and the in vivo behaviour of the examined fungi is discussed.