Cryopreservation of wheat (Triticum aestivum L.) egg cells by vitrification

• Autorzy: Fabian A. , Jager K. , Darko E. , Barnabas B.
• Języki publikacji: EN

Abstrakty

EN

A procedure has been developed for the cryopreservation of wheat female gametes. The procedure involves loading the cells with 25% concentrated vitrification solution consisting of 30% glycerol, 10% sucrose, 120 mM ascorbic acid (AA) and 5% propylene glycol (PG), dehydration in 80% concentrated vitrification solution, droplet vitrification and storage in liquid nitrogen, unloading and rehydration of the cells by gradual addition of isolation solution. Supplementation with AA significantly increased the proportion of viable egg cells after deand rehydration. During the early phase of rehydration AA reduced the probability of membrane damage caused by rapid water uptake. Maintaining the temperature of the cells at 0℃ during the de- and rehydration processes increased cell survival. Microscopic examination of the semi-thin sections of untreated and viable cryopreserved cells revealed that the vitrification process might cause changes in cell structure.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2008
• Tom: 30
• Numer: 5
• Opis fizyczny: p.737-744,fig.,ref.

Twórcy

autor

Fabian A.

• Agricultural Research Institute of the Hungarian Academy of Sciences, Brunszvik 2, 2462 Martonvasar, Hungary

autor

Jager K.

• Agricultural Research Institute of the Hungarian Academy of Sciences, Brunszvik 2, 2462 Martonvasar, Hungary

autor

Darko E.

• Agricultural Research Institute of the Hungarian Academy of Sciences, Brunszvik 2, 2462 Martonvasar, Hungary

autor

Barnabas B.

• Agricultural Research Institute of the Hungarian Academy of Sciences, Brunszvik 2, 2462 Martonvasar, Hungary

Bibliografia

• Agca Y, Monson R, Northey DL, Mazni OA, Schaefer DM, Rutledge JJ (1998) Transfer of fresh and cryopreserved IVP bovine embryos: normal calving, birth weight and gestation lengths. Theriogenology 50:147–162
• Amorim CA, Rondina D, Lucci CM, Goncalves PB, Figueiredo JR, Giorgetti A (2006) Permeability of ovine primordial follicles to different cryoprotectants. Fertil Steril 85(1):1077–1081
• Arrigoni O, De Tullio MC (2000) The role of ascorbic acid in cell metabolism: between gene-directed functions and unpredictable chemical reactions. J Plant Physiol 157:481–488
• Bajaj YPS (1995) Cryopreservation of plant cell, tissue organ culture for the conservation of germplasm and biodiversity. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 32 Cryopreservation of plant germplasm I. Springer, Berlin, pp 3–47
• Bakos F, Darkó É , Pónya Z, Barnabás B (2003) Regeneration of fertile wheat (Triticum aestivum L.) plants from isolated zygotes using wheat microspore culture as nurse cells. Plant Cell Tissue Organ Cult 74:243–247
• Barnabás B, Kovács G (1997) Storage of pollen. In: Shawhney UK, Shivanna KR (eds) Pollen biotechnology for crop production and improvement. Cambridge University Press, New York, pp 293–314
• Barnabás B, Kieft H, Schel JHN, Willemse MTM (1988) The ultrastructure of freeze-substituted maize pollen after cold storage. Ann Sci Univ Reims ARERS 23:100–103
• Blanco JM, Gee G, Wildt DE, Donoghue AM (2000) Species variation in osmotic, cryoprotectant, and cooling rate tolerance in poultry, eagle, and peregrine falcon spermatozoa. Biol Reprod 63:1164–1171
• Chen JL, Beversdorf WD (1992) Production of spontaneous diploid lines from isolated microspores following cryopreservation in spring rapeseed (Brassica napus L.). Plant Breed 108(4):324–327
• Chen Y, Wang JH (2003) Cryopreservation of carrot (Daucus carota L.) cell suspensions and protoplasts by vitrification. Cryo Lett 24(1):57–64
• Conklin P, Barth C (2004) Ascorbic acid, a familiar small molecule intertwined in the response of plants to ozone, pathogens and the onset of senescence. Plant Cell Environ 27:959–970
• De Gara L, Tommasi F (1999) Ascorbate redox enzymes: a network of reactions involved in plant development. Recent Res Dev Phytochem 3:1–15
• Dowgert MF, Steponkus PL (1984) Behavior of the plasma membrane of isolated protoplasts during a freeze-thaw cycle. Plant Physiol 75:1139–1151
• Dowgert MF, Wolfe J, Steponkus PL (1987) The mechanics of injury to isolated protoplasts following osmotic contraction and expansion. Plant Physiol 83(4):1001–1007
• Earnshaw BA, Johnson MA (1987) Control of wild carrot somatic embryo development by antioxidants: a probable mode of action of 2,4-dichlorophenoxyacetic acid. Plant Physiol 85:273–276
• Emiliani S, Van den Bergh M, Vannin AS, Biramane J, Englert Y (2000) Comparison of ethylene glycol, 1,2-propanediol, and glycerol for cryopreservation of slow-cooled mouse zygotes, 4-cell embryos, and blastocysts. Hum Reprod 15:905–910
• Fahy GM, McFarlane DR, Angell CA, Meryman HT (1984) Vitrification as an approach to cryopreservation. Cryobiology 21:407–426
• Finkle BJ, Ulrich JM (1979) Effects of cryoprotectants in combination on the survival of frozen sugarcane cells. Plant Physiol 63:598–604
• González-Reyes JA, Córdoba F, Navas P (1998) Involvement of plasma membrane redox systems in growth control of animal and plant cells. In: Asard H, Bérczi A, Caubergs RJ (eds) Plasma membrane redox systems and their role in biological stress and disease. Kluwer, Dordrecht, pp 193–213
• Henmi H, Endo T, Kitajima Y, Manase K, Hata H, Kudo R (2003) Effects of ascorbic acid supplementation on serum progesterone levels in patients with luteal phase defect. Fertil Steril 80:459–461
• Hoque AM, Akhter Banu NM, Okuma E, Amako K, Nakamura Y, Shimoishi Y, Murata Y (2007) Exogenous proline and glycinebetaine increase NaCl-induced ascorbate–glutathione cycle enzyme activities, and proline improves salt tolerance more than glycinebetaine in tobacco Bright Yellow-2 suspensioncultured cells. J Plant Physiol 164(11):1457–1468
• Huang CN, Wang JH, Yan QS, Zhang XQ, Yan QF (1995) Plantregeneration from rice (Oryza sativa L.) embryogenic suspension cells cryopreserved by vitrification. Plant Cell Rep 14(11):730–734
• Kartha KK (1985) Meristem culture and germplasm preservation. In: Kartha KK (ed) Cryopreservation of plant cells and organs. CRC Press, Boca Raton, pp 115–134
• Kovács M, Barnabás B, Kranz E (1994) The isolation of viable egg cells of wheat (Triticum aestivum L.). Sex Plant Reprod 7:311–312
• Kovács M, Barnabás B, Kranz E (1995) Electro-fused isolated wheat (Triticum aestivum L.) gametes develop into multicellular structures. Plant Cell Rep 15:178–180
• Lane M, Maybach JM, Gardner DK (2002) Addition of ascorbate during cryopreservation stimulates subsequent embryo development. Hum Reprod 17(10):2686–2693
• Langis R, Steponkus PL (1990) Cryopreservation of rye protoplasts by vitrification. Plant Physiol 92(3):666–671
• Liebler DC, Kling DS, Reed DJ (1986) Antioxidant protection of phospholipid bilayers by α-tocopherol. Control of α -tocopherol status by ascorbic acid and glutathione. J Biol Chem 261:12114–12119
• Montillet JL, Chamnongpol S, Rustérucci C, Dat J, Van de Cotte B, Agnel JP, Battesti C, Inzé D, Van Breusegem F, Triantaphylidés C (2005) Fatty acid hydroperoxides and H2O2 in the execution of hypersensitive cell death in tobacco leaves. Plant Physiol 138:1516–1526
• Nishizawa S, Sakai A, Amano Y, Matsuzawa T (1993) Cryopreservation of asparagus (Asparagus officinalis L.) embryogenic suspension cells and subsequent plant regeneration by vitrification. Plant Sci 91:67–73
• Packer JE, Slater TF, Wilson RL (1979) Direct observation of a free radical interaction between Vitamin E and Vitamin C. Nature 278:737–738
• Padh H (1990) Cellular functions of ascorbic acid. Biochem Cell Biol 68:1166–1173
• Panis B, Piette B, Swennen R (2005) Droplet vitrification of apical meristems: a cryopreservation protocol applicable to all Musaceae. Plant Sci 168:45–55
• Pedro PB, Yokoyoma E, Zhu SE, Yoshida N, Valdez DM, Tanaka M, Edashige K, Kasai M (2005) Permeability of mouse oocytes and embryos at various developmental stages to five cryoprotectants. J Reprod Dev 51:235–246
• Polle A (2001) Dissecting the superoxide dismutase-ascorbateglutathione- pathway in chloroplasts by metabolic modeling: computer simulations as a step towards flux analysis. Plant Physiol 126:445–462
• Pónya Z, Tímár I, Szabó L, Kristó f Z, Barnabás B (1999) Morphological characterization of wheat (T. aestivum L.) egg cell protoplasts isolated from immature and overaged caryopses. Sex Plant Reprod 11:357–359
• Potters G, Horemans N, Bellone S, Caubergs RJ, Trost P, Guisez Y, Asard H (2004) Dehydroascorbate influences the plant cell cycle through a glutathione-independent reduction mechanism. Plant Physiol 134(4):1479–1487
• Roeckel P, Dumas C (1993) Survival at 20℃ and cryopreservation of isolated sperm cells from Zea mays pollen grains. Sex Plant Reprod 6:212–216
• Sakai A (2000) Development of cryopreservation techniques. In: Engelmann F, Takagi H (eds) Cryopreservation of tropical plant germplasm. Current research progress and application. JIRCAS, IPGRI, Tsukuba, pp 1–8
• Sakai A, Kobayashi S, Oiyama I (1990) Cryopreservation of nucellar cells of navel orange (Citrus sinensis Osb. var. brasiliensis Tanaka) by vitrification. Plant Cell Rep 9:30–33
• Smirnoff N, Wheeler GL (2000) Ascorbic acid in plants: biosynthesis and function. Crit Rev Plant Sci 19:267–290
• Steponkus PL (1984) Role of the plasma membrane in freezing injury and cold acclimation. Ann Rev Plant Physiol Plant Mol Biol 35:543–584
• Sun K, Cui Y, Hauser BA (2005) Environmental stress alters genes expression and induces ovule abortion: reactive oxygen species appear as ovules commit to abort. Planta 222:632–642
• Tischner T, Kőszegi B, Veisz O (1997) Climatic programmes used in the Martonvásár phytotron most frequently in recent years. Acta Agron Hung 45:85–104
• Vidal N, Sánchez C, Jorquera L, Ballester A, Vieitez AM (2005) Cryopreservation of chestnut by vitrification of in vitro-grown shoot tips. In Vitro Cell Dev Biol Plant 41:63–68
• Wang YL, Fan MJ, Liaw SI (2005) Cryopreservation of in vitrogrown shoot tips of papaya (Carica papaya L.) by vitrification. Bot Bull Acad Sin 46:29–34
• Widholm JM (1972) The use of fluorescein diacetate and phenosafranine for determining viability of cultured plant cells. Stain Tech 47:189–194

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