Assessment of L-arginine as an effect of exogenous nitric oxide (NO) on expression of markers of cellular stress in rats' hepatocytes

• Autorzy: Pedrycz A. , Boratynski Z. , Orlowski M. , Siermontowski P.
• Języki publikacji: EN

Abstrakty

EN

The aim of this study was immunohistochemical evaluation of heat shock protein (Hsp70) and p-53 proteins in the L- arginine-induced cellular stress in hepatocvtes of rats. Sixteen white Wistar female rats were divided into two equal groups. The rats from the experimental group received per os 40 mg/kg b.w. of L-arginine every day for 2 weeks and were decapitated after 3 weeks of the experiment. The rats from the control group received in the same manner 2 ml of distilled water and were decapitated after 3 weeks of the experiment. After decapitation specimens from the liver were collected, fixed in 10% formalin, and then embedded in paraffin blocks. Proteins Hsp70 and p-53 were detected on slides using the standard three step immunohistochemical method. The quantitative evaluation of Hsp70 and p-53 expression showed that the area of positive staining in the liver of the experimental rats (Hsp70 305,763.00 µm² +/-58,289.66, p-53 9,551.42 µm² +/-l,078.86) was comparable to that in the control groups (Hsp70 291,636.80 µm² +/-34.492.31, p-53 14,104.67 µm² +/-3,571.35). Our experiment showed, that L-arginine as a precursor of exogenous nitric oxide given to rats in dose similar to that used in pregnant women treated for hypertension did not exhibit an influence on hepatocytes.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2012
• Tom: 56
• Numer: 1
• Opis fizyczny: p.89-92,fig.,ref.

Twórcy

autor

Pedrycz A.

• Laboratory of Experimental Cytology, Department of Histology and Embryology, Medical University of Lublin, 20-080 Lublin, Poland

autor

Boratynski Z.

• Department of Animal Anatomy and Histology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, 20-950 Lublin, Poland

autor

Orlowski M.

• Centrum of Diagnosis and Therapy of Digestive System Diseases, 80-807 Gdansk, Poland

autor

Siermontowski P.

• Department of Maritime and Hyperbaric Department, Medicine Military Institute of Medicine, 81-519 Gdynia, Poland

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