Theta oscillations in a novel in vitro preparation of posterior hypothalamus

• Autorzy: Kowalczyk T. , Golebiewski H. , Caban B. , Bocian R. , Kazmierska P. , Konopacki J.
• Języki publikacji: EN

Abstrakty

EN

Theta rhythm is the best synchronized EEG activity recorded from the mammalian brain. In rodents, this EEG pattern consists of high-voltage, regular, almost sinusoidal oscillations in the frequency range of 3-12 Hz. Hippocampal formation (HPC) is considered to be the main structure involved in the generation of this activity. Extensive anatomical and electrophysiological studies performed in rodents have revealed that the ascending brainstemhippocampal synchronizing pathway originates in the nucleus reticularis pontis oralis (RPO), next RPO fibres ascend to posterior hypothalamic (PH) region and then PH neurons projects to medial septal area which is well known as a hippocampal theta rhythm “pacemaker”. Earlier results suggest that the posterior hypothalamic region is only a modulator of hippocampal formation type II theta. Our preliminary studies suggest that this area is also capable of generation of local type II theta, which can be produced independently of the HPC theta rhythm. The purpose of the present study was to evoke the theta activity by tonic cholinergic (carbachol) or cholinergic/GABAergic (carbachol/bicuculine) bath perfusion in completely deaferentated posterior hypothalamus i.e. in novel PH slice preparations maintained in vitro. Experiments were performed on 25 posterior hypothalamic slices delivered from 25 Wistar rats. Slices were perfused with artificial cerebrospinal fluid containing carbachol (50 μM; 10 slices) or carbachol and bicuculine (50 μM and 10 μM respectively; 15 slices). Well synchronized hypothalamic theta activity was recorded in 7 carbachol-treated PH slices and in 13 PH slices perfused with carbachol/bicuculine.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2011
• Tom: 71
• Numer: S
• Opis fizyczny: p.43

Twórcy

autor

Kowalczyk T.

• Department of Neurobiology, University of Lodz, Lodz, Poland

autor

Golebiewski H.

• Department of Neurobiology, University of Lodz, Lodz, Poland

autor

Caban B.

• Department of Neurobiology, University of Lodz, Lodz, Poland

autor

Bocian R.

• Department of Neurobiology, University of Lodz, Lodz, Poland

autor

Kazmierska P.

• Department of Neurobiology, University of Lodz, Lodz, Poland

autor

Konopacki J.

• Department of Neurobiology, University of Lodz, Lodz, Poland

Uwagi

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